##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
AspEI AvrII BalI BcgI BciVI
BfrI BlnI BseAI BsiWI BsmI
BspDI BspEI BspLU11I Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI Eam1105I Eco47III Eco81I EcoO65I
EcoRI FseI MluNI MroI MscI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 175 GTGCAC G^TGCAC
ApaLI 175 GTGCAC G^TGCAC
Asp700I 4836 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1148 CACGTG CAC^GTG
BsaBI 4043 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3995 TCCGGT [AT]^CCGG[AT]
BsmBI 4665 CGTCTC CGTCTC(1/5)
Bsp1407I 2930 TGTACA T^GTACA
BspHI 4066 TCATGA T^CATGA
BspMI 3642 ACCTGC ACCTGC(4/8)
BsrGI 2930 TGTACA T^GTACA
BstXI 346 CCAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 4355 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 890 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3547 GATATC GAT^ATC
EcoT22I 5502 ATGCAT ATGCA^T
HincII 2249 GTTAAC GT[CT]^[GA]AC
HindII 2249 GTTAAC GT[CT]^[GA]AC
HpaI 2249 GTTAAC GTT^AAC
MamI 4043 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4142 CAATTG C^AATTG
MluI 2755 ACGCGT A^CGCGT
MunI 4142 CAATTG C^AATTG
NsiI 5502 ATGCAT ATGCA^T
PaeR7I 456 CTCGAG C^TCGAG
PflFI 3288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4895 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1148 CACGTG CAC^GTG
PmlI 1148 CACGTG CAC^GTG
RcaI 4066 TCATGA T^CATGA
SspBI 2930 TGTACA T^GTACA
Tth111I 3288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4895 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3530 CCACCCCAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 456 CTCGAG C^TCGAG
XmnI 4836 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 526 GGTACC G^GTACC
3187 GGTACC
AccI 1878 GTCTAC GT^[AC][GT]AC
5133 GTAGAC
AseI 1223 ATTAAT AT^TAAT
5216 ATTAAT
AsnI 1223 ATTAAT AT^TAAT
5216 ATTAAT
Asp718I 526 GGTACC G^GTACC
3187 GGTACC
AviII 589 TGCGCA TGC^GCA
892 TGCGCA
BclI 2318 TGATCA T^GATCA
4069 TGATCA
BglII 1739 AGATCT A^GATCT
2136 AGATCT
BlpI 941 GCTGAGC GC^T[ACGT]AGC
3279 GCTCAGC
Bpu1102I 941 GCTGAGC GC^T[ACGT]AGC
3279 GCTCAGC
BsaAI 1148 CACGTG [CT]AC^GT[GA]
1887 CACGTA
BsgI 4386 CTGCAC GTGCAG(16/14)
5207 CTGCAC
BsrDI 4193 CATTGC GCAATG(2/0)
4883 CATTGC
CelII 941 GCTGAGC GC^T[ACGT]AGC
3279 GCTCAGC
CpoI 448 CGGACCG CG^G[AT]CCG
2578 CGGTCCG
DraI 4151 TTTAAA TTT^AAA
5283 TTTAAA
DrdI 2666 GACAACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3458 GACCTGACAGTC
FbaI 2318 TGATCA T^GATCA
4069 TGATCA
FspI 589 TGCGCA TGC^GCA
892 TGCGCA
KpnI 526 GGTACC GGTAC^C
3187 GGTACC
NcoI 170 CCATGG C^CATGG
3539 CCATGG
PshBI 1223 ATTAAT AT^TAAT
5216 ATTAAT
RsrII 448 CGGACCG CG^G[AT]CCG
2578 CGGTCCG
SacI 944 GAGCTC GAGCT^C
1636 GAGCTC
SpeI 4528 ACTAGT A^CTAGT
4823 ACTAGT
SspI 3435 AATATT AAT^ATT
5175 AATATT
XbaI 1406 TCTAGA T^CTAGA
1850 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssHII BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI