################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AspEI AvrII BalI BcgI BciVI BfrI BlnI BseAI BsiWI BsmI BspDI BspEI BspLU11I Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI Eam1105I Eco47III Eco81I EcoO65I EcoRI FseI MluNI MroI MscI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI Psp1406I PvuI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Alw44I 175 GTGCAC G^TGCAC ApaLI 175 GTGCAC G^TGCAC Asp700I 4836 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 3288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1148 CACGTG CAC^GTG BsaBI 4043 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3995 TCCGGT [AT]^CCGG[AT] BsmBI 4665 CGTCTC CGTCTC(1/5) Bsp1407I 2930 TGTACA T^GTACA BspHI 4066 TCATGA T^CATGA BspMI 3642 ACCTGC ACCTGC(4/8) BsrGI 2930 TGTACA T^GTACA BstXI 346 CCAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 4355 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 890 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3547 GATATC GAT^ATC EcoT22I 5502 ATGCAT ATGCA^T HincII 2249 GTTAAC GT[CT]^[GA]AC HindII 2249 GTTAAC GT[CT]^[GA]AC HpaI 2249 GTTAAC GTT^AAC MamI 4043 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4142 CAATTG C^AATTG MluI 2755 ACGCGT A^CGCGT MunI 4142 CAATTG C^AATTG NsiI 5502 ATGCAT ATGCA^T PaeR7I 456 CTCGAG C^TCGAG PflFI 3288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4895 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1148 CACGTG CAC^GTG PmlI 1148 CACGTG CAC^GTG RcaI 4066 TCATGA T^CATGA SspBI 2930 TGTACA T^GTACA Tth111I 3288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4895 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3530 CCACCCCAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 456 CTCGAG C^TCGAG XmnI 4836 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 526 GGTACC G^GTACC 3187 GGTACC AccI 1878 GTCTAC GT^[AC][GT]AC 5133 GTAGAC AseI 1223 ATTAAT AT^TAAT 5216 ATTAAT AsnI 1223 ATTAAT AT^TAAT 5216 ATTAAT Asp718I 526 GGTACC G^GTACC 3187 GGTACC AviII 589 TGCGCA TGC^GCA 892 TGCGCA BclI 2318 TGATCA T^GATCA 4069 TGATCA BglII 1739 AGATCT A^GATCT 2136 AGATCT BlpI 941 GCTGAGC GC^T[ACGT]AGC 3279 GCTCAGC Bpu1102I 941 GCTGAGC GC^T[ACGT]AGC 3279 GCTCAGC BsaAI 1148 CACGTG [CT]AC^GT[GA] 1887 CACGTA BsgI 4386 CTGCAC GTGCAG(16/14) 5207 CTGCAC BsrDI 4193 CATTGC GCAATG(2/0) 4883 CATTGC CelII 941 GCTGAGC GC^T[ACGT]AGC 3279 GCTCAGC CpoI 448 CGGACCG CG^G[AT]CCG 2578 CGGTCCG DraI 4151 TTTAAA TTT^AAA 5283 TTTAAA DrdI 2666 GACAACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3458 GACCTGACAGTC FbaI 2318 TGATCA T^GATCA 4069 TGATCA FspI 589 TGCGCA TGC^GCA 892 TGCGCA KpnI 526 GGTACC GGTAC^C 3187 GGTACC NcoI 170 CCATGG C^CATGG 3539 CCATGG PshBI 1223 ATTAAT AT^TAAT 5216 ATTAAT RsrII 448 CGGACCG CG^G[AT]CCG 2578 CGGTCCG SacI 944 GAGCTC GAGCT^C 1636 GAGCTC SpeI 4528 ACTAGT A^CTAGT 4823 ACTAGT SspI 3435 AATATT AAT^ATT 5175 AATATT XbaI 1406 TCTAGA T^CTAGA 1850 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BamHI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI