##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AvrII BalI BamHI
BanI BbeI BbrPI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiWI BspEI BssHII BssSI
BstEII BstPI BstXI CelII CpoI
DrdI Eco47III EcoNI EcoO65I FauI
FseI Hin1I HindIII KasI KpnI
MamI MluI MluNI MroI MscI
NarI NcoI NdeI NheI NruI
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshAI RsrII SacI SbfI
ScaI SexAI SfoI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Van91I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AclI 666 AACGTT AA^CGTT
AflIII 558 ACATGT A^C[GA][CT]GT
AseI 897 ATTAAT AT^TAAT
AsnI 897 ATTAAT AT^TAAT
AspI 1460 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 4354 CCTCAGC CCTCAGC(-5/-2)
BcgI 1270 GCACCATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4608 GTATCC GTATCC(6/5)
BclI 4588 TGATCA T^GATCA
BsgI 1366 CTGCAC GTGCAG(16/14)
BspDI 460 ATCGAT AT^CGAT
BspLU11I 558 ACATGT A^CATGT
BsrBI 981 CCGCTC CCGCTC(-3/-3)
BstAPI 420 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 460 ATCGAT AT^CGAT
DraIII 686 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 4648 GATATC GAT^ATC
EcoT22I 4725 ATGCAT ATGCA^T
FbaI 4588 TGATCA T^GATCA
NaeI 59 GCCGGC GCC^GGC
NgoMIV 59 GCCGGC G^CCGGC
NotI 1440 GCGGCCGC GC^GGCCGC
NsiI 4725 ATGCAT ATGCA^T
PflFI 1460 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1010 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 897 ATTAAT AT^TAAT
Psp1406I 666 AACGTT AA^CGTT
PvuI 462 CGATCG CGAT^CG
SalI 1466 GTCGAC G^TCGAC
SapI 1530 GCTCTTC GCTCTTC(1/4)
SfiI 1415 GGCCGCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 1460 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 785 CCAGGTTGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AgeI 1112 ACCGGT A^CCGGT
1136 ACCGGT
AhdI 2187 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2993 GACGGACAGTC
AlwNI 1617 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2423 CAGGCTCTG
AocI 1824 CCTAAGG CC^T[ACGT]AGG
2630 CCTAAGG
AspEI 2187 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2993 GACGGACAGTC
AvaI 1148 CCCGGG C^[CT]CG[GA]G
1228 CCCGGG
AvaII 152 GGTCC G^G[AT]CC
1011 GGTCC
BsaI 1907 GAGACC GGTCTC(1/5)
2713 GAGACC
BsmBI 2185 GAGACG CGTCTC(1/5)
2991 GAGACG
BsoBI 1148 CCCGGG C^[CT]CG[GA]G
1228 CCCGGG
BsrDI 2347 CATTGC GCAATG(2/0)
3153 CATTGC
Bst1107I 1876 GTATAC GTA^TAC
2682 GTATAC
BstBI 1923 TTCGAA TT^CGAA
2729 TTCGAA
BstZ17I 1876 GTATAC GTA^TAC
2682 GTATAC
Bsu36I 1824 CCTAAGG CC^T[ACGT]AGG
2630 CCTAAGG
DraII 453 GGGGCCT [GA]G^G[ACGT]CC[CT]
1010 AGGTCCC
EagI 57 CGGCCG C^GGCCG
1441 CGGCCG
Eam1105I 2187 GACGGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2993 GACGGACAGTC
EclXI 57 CGGCCG C^GGCCG
1441 CGGCCG
Eco52I 57 CGGCCG C^GGCCG
1441 CGGCCG
Eco81I 1824 CCTAAGG CC^T[ACGT]AGG
2630 CCTAAGG
EcoO109I 453 GGGGCCT [GA]G^G[ACGT]CC[CT]
1010 AGGTCCC
EcoRI 533 GAATTC G^AATTC
5111 GAATTC
EcoT14I 2104 CCAAGG C^C[AT][AT]GG
2910 CCAAGG
HaeII 237 AGCGCC [GA]GCGC^[CT]
1058 AGCGCC
HpaI 1896 GTTAAC GTT^AAC
2702 GTTAAC
MfeI 2268 CAATTG C^AATTG
3074 CAATTG
MunI 2268 CAATTG C^AATTG
3074 CAATTG
NspV 1923 TTCGAA TT^CGAA
2729 TTCGAA
PinAI 1112 ACCGGT A^CCGGT
1136 ACCGGT
SfuI 1923 TTCGAA TT^CGAA
2729 TTCGAA
SmaI 1148 CCCGGG CCC^GGG
1228 CCCGGG
SphI 1788 GCATGC GCATG^C
2594 GCATGC
StyI 2104 CCAAGG C^C[AT][AT]GG
2910 CCAAGG
XbaI 2337 TCTAGA T^CTAGA
3143 TCTAGA
XmaCI 1148 CCCGGG C^CCGGG
1228 CCCGGG
XmaI 1148 CCCGGG C^CCGGG
1228 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AspHI AviII
BanII BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspMI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRII Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII