##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI ApaI AscI AspEI
AviII AvrII BamHI BbeI BbrPI
BcgI BlnI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BssHII BssSI Bst1107I BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI FseI FspI
Hin1I HindIII KasI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 4476 ACATGT A^C[GA][CT]GT
AseI 1750 ATTAAT AT^TAAT
AsnI 1750 ATTAAT AT^TAAT
AvaI 1340 CCCGAG C^[CT]CG[GA]G
BciVI 735 GGATAC GTATCC(6/5)
BglI 2107 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2677 AGATCT A^GATCT
BsaAI 2123 TACGTG [CT]AC^GT[GA]
BsaBI 1261 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1340 CCCGAG C^[CT]CG[GA]G
BspLU11I 4476 ACATGT A^CATGT
BstYI 2677 AGATCT [GA]^GATC[CT]
DrdI 3116 GACAATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2666 GATATC GAT^ATC
HincII 1621 GTTAAC GT[CT]^[GA]AC
HindII 1621 GTTAAC GT[CT]^[GA]AC
HpaI 1621 GTTAAC GTT^AAC
MamI 1261 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1988 CAATTG C^AATTG
MflI 2677 AGATCT [GA]^GATC[CT]
MspA1I 4459 CAGCGG C[AC]G^C[GT]G
MunI 1988 CAATTG C^AATTG
PshAI 2209 GACTTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1750 ATTAAT AT^TAAT
ScaI 1007 AGTACT AGT^ACT
SexAI 1811 ACCTGGT A^CC[AT]GGT
SspI 1294 AATATT AAT^ATT
XbaI 576 TCTAGA T^CTAGA
XhoII 2677 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 439 GTCTAC GT^[AC][GT]AC
1563 GTCTAC
AflII 165 CTTAAG C^TTAAG
2211 CTTAAG
Alw44I 4911 GTGCAC G^TGCAC
5796 GTGCAC
AocI 2747 CCTCAGG CC^T[ACGT]AGG
5280 CCTCAGG
ApaLI 4911 GTGCAC G^TGCAC
5796 GTGCAC
AspI 433 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6423 GACTGGGTC
BcnI 3213 CCCGG CC^[GC]GG
3364 CCGGG
BfrI 165 CTTAAG C^TTAAG
2211 CTTAAG
BlpI 748 GCTGAGC GC^T[ACGT]AGC
1042 GCTAAGC
Bpu1102I 748 GCTGAGC GC^T[ACGT]AGC
1042 GCTAAGC
BsmI 4974 GAATGC GAATGC(1/-1)
5990 GCATTC
BsrBI 133 GAGCGG CCGCTC(-3/-3)
712 GAGCGG
BsrFI 2060 GCCGGT [GA]^CCGG[CT]
3828 ACCGGC
BstAPI 3970 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4779 GCAGTAACTGC
BstBI 1859 TTCGAA TT^CGAA
3171 TTCGAA
BstEII 4379 GGTAACC G^GT[ACGT]ACC
5922 GGTGACC
BstPI 4379 GGTAACC G^GT[ACGT]ACC
5922 GGTGACC
Bsu36I 2747 CCTCAGG CC^T[ACGT]AGG
5280 CCTCAGG
CelII 748 GCTGAGC GC^T[ACGT]AGC
1042 GCTAAGC
Cfr10I 2060 GCCGGT [GA]^CCGG[CT]
3828 ACCGGC
DraI 1102 TTTAAA TTT^AAA
2327 TTTAAA
Eco81I 2747 CCTCAGG CC^T[ACGT]AGG
5280 CCTCAGG
EcoO65I 4379 GGTAACC G^GT[ACGT]ACC
5922 GGTGACC
EcoT22I 3316 ATGCAT ATGCA^T
5988 ATGCAT
FauI 1578 GCGGG CCCGC(4/6)
4461 GCGGG
HaeII 2185 GGCGCT [GA]GCGC^[CT]
4116 AGCGCC
NciI 3213 CCCGG CC^[GC]GG
3364 CCGGG
NdeI 2369 CATATG CA^TATG
3132 CATATG
NsiI 3316 ATGCAT ATGCA^T
5988 ATGCAT
NspV 1859 TTCGAA TT^CGAA
3171 TTCGAA
PflFI 433 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6423 GACTGGGTC
SfuI 1859 TTCGAA TT^CGAA
3171 TTCGAA
SphI 3522 GCATGC GCATG^C
3577 GCATGC
Tth111I 433 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
6423 GACTGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
Asp718I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BclI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmnI