##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AgeI AocI Aor51HI
AscI AvrII BbeI BciVI BglI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BssHII BssSI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI KasI MluI MroI
NarI NotI NruI NspV PacI
PaeR7I PinAI PmeI PvuI RsrII
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4086 GGTACC G^GTACC
AcyI 78 GACGCC G[GA]^CG[CT]C
AflII 5592 CTTAAG C^TTAAG
AflIII 5560 ACGTGT A^C[GA][CT]GT
AhdI 4566 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1908 GGGCCC GGGCC^C
Asp718I 4086 GGTACC G^GTACC
AspEI 4566 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 5520 CACGTG CAC^GTG
BclI 2752 TGATCA T^GATCA
BfrI 5592 CTTAAG C^TTAAG
BglII 5588 AGATCT A^GATCT
BsaHI 78 GACGCC G[GA]^CG[CT]C
Bsp1407I 5668 TGTACA T^GTACA
BspHI 2118 TCATGA T^CATGA
BsrBI 1778 GAGCGG CCGCTC(-3/-3)
BsrGI 5668 TGTACA T^GTACA
Bst1107I 5027 GTATAC GTA^TAC
BstZ17I 5027 GTATAC GTA^TAC
Eam1105I 4566 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4556 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 5616 GAATTC G^AATTC
EcoRV 1916 GATATC GAT^ATC
FbaI 2752 TGATCA T^GATCA
Hin1I 78 GACGCC G[GA]^CG[CT]C
KpnI 4086 GGTACC GGTAC^C
KspI 62 CCGCGG CCGC^GG
NdeI 1899 CATATG CA^TATG
NheI 4296 GCTAGC G^CTAGC
PmaCI 5520 CACGTG CAC^GTG
PmlI 5520 CACGTG CAC^GTG
PshAI 747 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2118 TCATGA T^CATGA
SacI 1642 GAGCTC GAGCT^C
SacII 62 CCGCGG CCGC^GG
ScaI 1059 AGTACT AGT^ACT
SspBI 5668 TGTACA T^GTACA
SspI 4242 AATATT AAT^ATT
StuI 1935 AGGCCT AGG^CCT
XbaI 3745 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 21 CGCG CG^CG
63 CGCG
AclI 1049 AACGTT AA^CGTT
5567 AACGTT
Alw44I 3678 GTGCAC G^TGCAC
5523 GTGCAC
ApaLI 3678 GTGCAC G^TGCAC
5523 GTGCAC
AseI 422 ATTAAT AT^TAAT
4128 ATTAAT
AsnI 422 ATTAAT AT^TAAT
4128 ATTAAT
Asp700I 4883 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5051 GAAGGCTTTC
AviII 1141 TGCGCA TGC^GCA
2824 TGCGCA
BamHI 3608 GGATCC G^GATCC
5082 GGATCC
BanI 4086 GGTACC G^G[CT][GA]CC
4913 GGTGCC
BanII 1642 GAGCTC G[GA]GC[CT]^C
1908 GGGCCC
BsaBI 2475 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3778 GATCCTCATC
BsmI 2011 GAATGC GAATGC(1/-1)
2528 GCATTC
BstUI 21 CGCG CG^CG
63 CGCG
DraI 246 TTTAAA TTT^AAA
4218 TTTAAA
EcoT22I 193 ATGCAT ATGCA^T
4180 ATGCAT
FauI 1947 CCCGC CCCGC(4/6)
661 GCGGG
FspI 1141 TGCGCA TGC^GCA
2824 TGCGCA
HaeII 46 GGCGCT [GA]GCGC^[CT]
66 GGCGCT
HpaI 4226 GTTAAC GTT^AAC
5570 GTTAAC
MamI 2475 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3778 GATCCTCATC
MfeI 2387 CAATTG C^AATTG
5118 CAATTG
MunI 2387 CAATTG C^AATTG
5118 CAATTG
MvnI 21 CGCG CG^CG
63 CGCG
NaeI 43 GCCGGC GCC^GGC
81 GCCGGC
NcoI 3612 CCATGG C^CATGG
4344 CCATGG
NgoMIV 43 GCCGGC G^CCGGC
81 GCCGGC
NsiI 193 ATGCAT ATGCA^T
4180 ATGCAT
PshBI 422 ATTAAT AT^TAAT
4128 ATTAAT
Psp1406I 1049 AACGTT AA^CGTT
5567 AACGTT
SalI 744 GTCGAC G^TCGAC
5339 GTCGAC
SmaI 1911 CCCGGG CCC^GGG
4922 CCCGGG
XmaCI 1911 CCCGGG C^CCGGG
4922 CCCGGG
XmaI 1911 CCCGGG C^CCGGG
4922 CCCGGG
XmnI 4883 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5051 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BbsI BbvCI
BbvI BcgI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII