##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspI AvaI AviII BbeI BbrPI
BbvCI BcgI BclI BfrI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MaeII MluI MroI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 94 GTCTAC GT^[AC][GT]AC
AhdI 86 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 344 ATTAAT AT^TAAT
AsnI 344 ATTAAT AT^TAAT
AspEI 86 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3296 TGGCCA TGG^CCA
BanI 2360 GGTGCC G^G[CT][GA]CC
BglI 3451 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2095 AGATCT A^GATCT
CfoI 2125 GCGC GCG^C
DraII 2643 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 258 CCATGG C^C[GA][CT]GG
EaeI 3296 TGGCCA [CT]^GGCC[GA]
Eam1105I 86 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2643 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1806 GAATTC G^AATTC
EcoRV 2171 GATATC GAT^ATC
EcoT22I 2673 ATGCAT ATGCA^T
FauI 2545 GCGGG CCCGC(4/6)
HaeII 2124 AGCGCC [GA]GCGC^[CT]
HhaI 2125 GCGC GCG^C
HinP1I 2125 GCGC G^CGC
HpaI 4622 GTTAAC GTT^AAC
MfeI 2855 CAATTG C^AATTG
MluNI 3296 TGGCCA TGG^CCA
MscI 3296 TGGCCA TGG^CCA
MunI 2855 CAATTG C^AATTG
NcoI 258 CCATGG C^CATGG
NdeI 1161 CATATG CA^TATG
NsiI 2673 ATGCAT ATGCA^T
PacI 4381 TTAATTAA TTAAT^TAA
PpuMI 2643 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2057 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 344 ATTAAT AT^TAAT
SexAI 3170 ACCTGGT A^CC[AT]GGT
XbaI 1303 TCTAGA T^CTAGA
XcmI 3078 CCAAACTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 2644 GGACC G^G[AT]CC
3328 GGACC
AvrII 3341 CCTAGG C^CTAGG
3675 CCTAGG
BamHI 250 GGATCC G^GATCC
1526 GGATCC
BcnI 510 CCCGG CC^[GC]GG
2779 CCGGG
BlnI 3341 CCTAGG C^CTAGG
3675 CCTAGG
BlpI 2985 GCTCAGC GC^T[ACGT]AGC
3789 GCTGAGC
Bpu1102I 2985 GCTCAGC GC^T[ACGT]AGC
3789 GCTGAGC
BsaBI 538 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1012 GATTGTTATC
BstXI 2889 CCAATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3514 CCAATGTATTGG
CelII 2985 GCTCAGC GC^T[ACGT]AGC
3789 GCTGAGC
MamI 538 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1012 GATTGTTATC
NciI 510 CCCGG CC^[GC]GG
2779 CCGGG
NheI 232 GCTAGC G^CTAGC
1762 GCTAGC
PstI 3083 CTGCAG CTGCA^G
3390 CTGCAG
SpeI 2614 ACTAGT A^CTAGT
4493 ACTAGT
SspI 4 AATATT AAT^ATT
4770 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
BanII BbsI BbvI BciVI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI