##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspI        AvaI        AviII       BbeI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BsaAI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     FbaI        FseI        FspI        HgaI        
Hin1I       HindIII     KasI        KpnI        KspI        
MaeII       MluI        MroI        MvnI        NaeI        
NarI        NgoMIV      NotI        NruI        NspV        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SwaI        Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI           94  GTCTAC               GT^[AC][GT]AC
AhdI           86  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI          344  ATTAAT               AT^TAAT
AsnI          344  ATTAAT               AT^TAAT
AspEI          86  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         3296  TGGCCA               TGG^CCA
BanI         2360  GGTGCC               G^G[CT][GA]CC
BglI         3451  GCCATTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2095  AGATCT               A^GATCT
CfoI         2125  GCGC                 GCG^C
DraII        2643  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          258  CCATGG               C^C[GA][CT]GG
EaeI         3296  TGGCCA               [CT]^GGCC[GA]
Eam1105I       86  GACTCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I     2643  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1806  GAATTC               G^AATTC
EcoRV        2171  GATATC               GAT^ATC
EcoT22I      2673  ATGCAT               ATGCA^T
FauI         2545  GCGGG                CCCGC(4/6)
HaeII        2124  AGCGCC               [GA]GCGC^[CT]
HhaI         2125  GCGC                 GCG^C
HinP1I       2125  GCGC                 G^CGC
HpaI         4622  GTTAAC               GTT^AAC
MfeI         2855  CAATTG               C^AATTG
MluNI        3296  TGGCCA               TGG^CCA
MscI         3296  TGGCCA               TGG^CCA
MunI         2855  CAATTG               C^AATTG
NcoI          258  CCATGG               C^CATGG
NdeI         1161  CATATG               CA^TATG
NsiI         2673  ATGCAT               ATGCA^T
PacI         4381  TTAATTAA             TTAAT^TAA
PpuMI        2643  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2057  GACTATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         344  ATTAAT               AT^TAAT
SexAI        3170  ACCTGGT              A^CC[AT]GGT
XbaI         1303  TCTAGA               T^CTAGA
XcmI         3078  CCAAACTGCAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AvaII        2644  GGACC                G^G[AT]CC
             3328  GGACC               
AvrII        3341  CCTAGG               C^CTAGG
             3675  CCTAGG              
BamHI         250  GGATCC               G^GATCC
             1526  GGATCC              
BcnI          510  CCCGG                CC^[GC]GG
             2779  CCGGG               
BlnI         3341  CCTAGG               C^CTAGG
             3675  CCTAGG              
BlpI         2985  GCTCAGC              GC^T[ACGT]AGC
             3789  GCTGAGC             
Bpu1102I     2985  GCTCAGC              GC^T[ACGT]AGC
             3789  GCTGAGC             
BsaBI         538  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1012  GATTGTTATC          
BstXI        2889  CCAATAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3514  CCAATGTATTGG        
CelII        2985  GCTCAGC              GC^T[ACGT]AGC
             3789  GCTGAGC             
MamI          538  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1012  GATTGTTATC          
NciI          510  CCCGG                CC^[GC]GG
             2779  CCGGG               
NheI          232  GCTAGC               G^CTAGC
             1762  GCTAGC              
PstI         3083  CTGCAG               CTGCA^G
             3390  CTGCAG              
SpeI         2614  ACTAGT               A^CTAGT
             4493  ACTAGT              
SspI            4  AATATT               AAT^ATT
             4770  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
BanII       BbsI        BbvI        BciVI       BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EarI        EcoNI       EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI