################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspI AvaI AviII BbeI BbrPI BbvCI BcgI BclI BfrI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I FbaI FseI FspI HgaI Hin1I HindIII KasI KpnI KspI MaeII MluI MroI MvnI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 94 GTCTAC GT^[AC][GT]AC AhdI 86 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 344 ATTAAT AT^TAAT AsnI 344 ATTAAT AT^TAAT AspEI 86 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 3296 TGGCCA TGG^CCA BanI 2360 GGTGCC G^G[CT][GA]CC BglI 3451 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2095 AGATCT A^GATCT CfoI 2125 GCGC GCG^C DraII 2643 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 258 CCATGG C^C[GA][CT]GG EaeI 3296 TGGCCA [CT]^GGCC[GA] Eam1105I 86 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 2643 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1806 GAATTC G^AATTC EcoRV 2171 GATATC GAT^ATC EcoT22I 2673 ATGCAT ATGCA^T FauI 2545 GCGGG CCCGC(4/6) HaeII 2124 AGCGCC [GA]GCGC^[CT] HhaI 2125 GCGC GCG^C HinP1I 2125 GCGC G^CGC HpaI 4622 GTTAAC GTT^AAC MfeI 2855 CAATTG C^AATTG MluNI 3296 TGGCCA TGG^CCA MscI 3296 TGGCCA TGG^CCA MunI 2855 CAATTG C^AATTG NcoI 258 CCATGG C^CATGG NdeI 1161 CATATG CA^TATG NsiI 2673 ATGCAT ATGCA^T PacI 4381 TTAATTAA TTAAT^TAA PpuMI 2643 AGGACCT [GA]G^G[AT]CC[CT] PshAI 2057 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 344 ATTAAT AT^TAAT SexAI 3170 ACCTGGT A^CC[AT]GGT XbaI 1303 TCTAGA T^CTAGA XcmI 3078 CCAAACTGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AvaII 2644 GGACC G^G[AT]CC 3328 GGACC AvrII 3341 CCTAGG C^CTAGG 3675 CCTAGG BamHI 250 GGATCC G^GATCC 1526 GGATCC BcnI 510 CCCGG CC^[GC]GG 2779 CCGGG BlnI 3341 CCTAGG C^CTAGG 3675 CCTAGG BlpI 2985 GCTCAGC GC^T[ACGT]AGC 3789 GCTGAGC Bpu1102I 2985 GCTCAGC GC^T[ACGT]AGC 3789 GCTGAGC BsaBI 538 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1012 GATTGTTATC BstXI 2889 CCAATAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3514 CCAATGTATTGG CelII 2985 GCTCAGC GC^T[ACGT]AGC 3789 GCTGAGC MamI 538 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1012 GATTGTTATC NciI 510 CCCGG CC^[GC]GG 2779 CCGGG NheI 232 GCTAGC G^CTAGC 1762 GCTAGC PstI 3083 CTGCAG CTGCA^G 3390 CTGCAG SpeI 2614 ACTAGT A^CTAGT 4493 ACTAGT SspI 4 AATATT AAT^ATT 4770 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI BanII BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI