##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI BclI BglII BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MfeI MluI
MunI NdeI NotI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 313 TCCGGA T^CCGGA
AclI 3863 AACGTT AA^CGTT
AcsI 3889 AAATTT [GA]^AATT[CT]
AflIII 586 ACACGT A^C[GA][CT]GT
AhdI 2351 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3889 AAATTT [GA]^AATT[CT]
AspEI 2351 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1200 TGCGCA TGC^GCA
BbeI 2427 GGCGCC GGCGC^C
BsaBI 1534 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 313 TCCGGA T^CCGGA
BspEI 313 TCCGGA T^CCGGA
BspHI 822 TCATGA T^CATGA
BsrDI 2346 GCAATG GCAATG(2/0)
BssSI 2937 CACGAG CACGAG(-5/-1)
DraIII 2394 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2351 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1200 TGCGCA TGC^GCA
HincII 1745 GTCAAC GT[CT]^[GA]AC
HindII 1745 GTCAAC GT[CT]^[GA]AC
KasI 2427 GGCGCC G^GCGCC
MamI 1534 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 313 TCCGGA T^CCGGA
NarI 2427 GGCGCC GG^CGCC
NheI 214 GCTAGC G^CTAGC
NruI 2714 TCGCGA TCG^CGA
NspI 3594 GCATGT [GA]CATG^[CT]
Psp1406I 3863 AACGTT AA^CGTT
RcaI 822 TCATGA T^CATGA
SacI 1265 GAGCTC GAGCT^C
ScaI 1171 AGTACT AGT^ACT
SexAI 3132 ACCAGGT A^CC[AT]GGT
SfiI 1793 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2427 GGCGCC GGC^GCC
SpeI 457 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 2715 CGCG CG^CG
5050 CGCG
AcyI 1460 GGCGTC G[GA]^CG[CT]C
2427 GGCGCC
AflII 2737 CTTAAG C^TTAAG
4057 CTTAAG
Alw44I 1016 GTGCAC G^TGCAC
1312 GTGCAC
ApaLI 1016 GTGCAC G^TGCAC
1312 GTGCAC
BbsI 1660 GAAGAC GAAGAC(2/6)
4736 GTCTTC
BciVI 103 GGATAC GTATCC(6/5)
2833 GGATAC
BfrI 2737 CTTAAG C^TTAAG
4057 CTTAAG
BpuAI 1660 GAAGAC GAAGAC(2/6)
4736 GTCTTC
BsaHI 1460 GGCGTC G[GA]^CG[CT]C
2427 GGCGCC
BsaWI 313 TCCGGA [AT]^CCGG[AT]
832 TCCGGT
BsmI 1395 GAATGC GAATGC(1/-1)
1834 GAATGC
BsrBI 2014 CCGCTC CCGCTC(-3/-3)
1097 GAGCGG
BsrFI 2132 GCCGGC [GA]^CCGG[CT]
2638 GCCGGC
BstUI 2715 CGCG CG^CG
5050 CGCG
Cfr10I 2132 GCCGGC [GA]^CCGG[CT]
2638 GCCGGC
Hin1I 1460 GGCGTC G[GA]^CG[CT]C
2427 GGCGCC
HphI 145 GGTGA GGTGA(8/7)
3404 TCACC
MvnI 2715 CGCG CG^CG
5050 CGCG
NaeI 2132 GCCGGC GCC^GGC
2638 GCCGGC
NgoMIV 2132 GCCGGC G^CCGGC
2638 GCCGGC
PaeR7I 2268 CTCGAG C^TCGAG
3712 CTCGAG
PflMI 1798 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3206 CCAGCCAGTGG
SapI 3501 GAAGAGC GCTCTTC(1/4)
1819 GCTCTTC
StuI 606 AGGCCT AGG^CCT
4933 AGGCCT
Tsp509I 3890 AATT ^AATT
5034 AATT
Van91I 1798 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3206 CCAGCCAGTGG
XhoI 2268 CTCGAG C^TCGAG
3712 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbrPI BbvCI BbvI BcgI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI