################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII AclI AflII AgeI AscI AseI AsnI Asp700I BciVI BfrI BglII BsiWI BspDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EcoNI EcoRV HindIII HpaI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I RsrII SbfI ScaI SexAI SfuI SgrAI SnaBI Sse8387I SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 2660 GGTACC G^GTACC AccIII 1464 TCCGGA T^CCGGA AcsI 254 GAATTC [GA]^AATT[CT] AhdI 2122 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 254 GAATTC [GA]^AATT[CT] Asp718I 2660 GGTACC G^GTACC AspEI 2122 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 4118 TGCGCA TGC^GCA BamHI 777 GGATCC G^GATCC BcgI 2746 GCAGCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 344 TGATCA T^GATCA BsaBI 2805 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1464 TCCGGA T^CCGGA BspEI 1464 TCCGGA T^CCGGA BspHI 3428 TCATGA T^CATGA BspLU11I 4859 ACATGT A^CATGT BsrDI 396 CATTGC GCAATG(2/0) BstEII 3393 GGTCACC G^GT[ACGT]ACC BstPI 3393 GGTCACC G^GT[ACGT]ACC DraI 5309 TTTAAA TTT^AAA DraIII 520 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2122 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3393 GGTCACC G^GT[ACGT]ACC EcoRI 254 GAATTC G^AATTC EcoT22I 4869 ATGCAT ATGCA^T FbaI 344 TGATCA T^GATCA FseI 3754 GGCCGGCC GGCCGG^CC FspI 4118 TGCGCA TGC^GCA KpnI 2660 GGTACC GGTAC^C KspI 2582 CCGCGG CCGC^GG MamI 2805 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1464 TCCGGA T^CCGGA NsiI 4869 ATGCAT ATGCA^T PaeR7I 3198 CTCGAG C^TCGAG PshAI 979 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 4224 CGATCG CGAT^CG RcaI 3428 TCATGA T^CATGA SacII 2582 CCGCGG CCGC^GG SalI 5505 GTCGAC G^TCGAC SapI 423 GAAGAGC GCTCTTC(1/4) SmaI 2133 CCCGGG CCC^GGG SpeI 4814 ACTAGT A^CTAGT XhoI 3198 CTCGAG C^TCGAG XmaCI 2133 CCCGGG C^CCGGG XmaI 2133 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AocI 1471 CCTGAGG CC^T[ACGT]AGG 5538 CCTAAGG Aor51HI 76 AGCGCT AGC^GCT 2666 AGCGCT AvaI 2133 CCCGGG C^[CT]CG[GA]G 3198 CTCGAG AvrII 1734 CCTAGG C^CTAGG 4534 CCTAGG BbeI 2906 GGCGCC GGCGC^C 4723 GGCGCC BbvCI 2591 CCTCAGC CCTCAGC(-5/-2) 2370 GCTGAGG BlnI 1734 CCTAGG C^CTAGG 4534 CCTAGG BsmBI 1797 CGTCTC CGTCTC(1/5) 3495 CGTCTC BsoBI 2133 CCCGGG C^[CT]CG[GA]G 3198 CTCGAG BssHII 3687 GCGCGC G^CGCGC 3906 GCGCGC Bsu36I 1471 CCTGAGG CC^T[ACGT]AGG 5538 CCTAAGG EagI 3753 CGGCCG C^GGCCG 4609 CGGCCG EclXI 3753 CGGCCG C^GGCCG 4609 CGGCCG Eco47III 76 AGCGCT AGC^GCT 2666 AGCGCT Eco52I 3753 CGGCCG C^GGCCG 4609 CGGCCG Eco81I 1471 CCTGAGG CC^T[ACGT]AGG 5538 CCTAAGG KasI 2906 GGCGCC G^GCGCC 4723 GGCGCC NaeI 3755 GCCGGC GCC^GGC 4313 GCCGGC NarI 2906 GGCGCC GG^CGCC 4723 GGCGCC NgoMIV 3755 GCCGGC G^CCGGC 4313 GCCGGC PstI 135 CTGCAG CTGCA^G 4009 CTGCAG SacI 5063 GAGCTC GAGCT^C 5671 GAGCTC SfoI 2906 GGCGCC GGC^GCC 4723 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 141 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AspI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII