##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI Asp700I BciVI BfrI
BglII BsiWI BspDI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EcoNI EcoRV HindIII HpaI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I RsrII SbfI ScaI
SexAI SfuI SgrAI SnaBI Sse8387I
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 2660 GGTACC G^GTACC
AccIII 1464 TCCGGA T^CCGGA
AcsI 254 GAATTC [GA]^AATT[CT]
AhdI 2122 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 254 GAATTC [GA]^AATT[CT]
Asp718I 2660 GGTACC G^GTACC
AspEI 2122 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4118 TGCGCA TGC^GCA
BamHI 777 GGATCC G^GATCC
BcgI 2746 GCAGCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 344 TGATCA T^GATCA
BsaBI 2805 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1464 TCCGGA T^CCGGA
BspEI 1464 TCCGGA T^CCGGA
BspHI 3428 TCATGA T^CATGA
BspLU11I 4859 ACATGT A^CATGT
BsrDI 396 CATTGC GCAATG(2/0)
BstEII 3393 GGTCACC G^GT[ACGT]ACC
BstPI 3393 GGTCACC G^GT[ACGT]ACC
DraI 5309 TTTAAA TTT^AAA
DraIII 520 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2122 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3393 GGTCACC G^GT[ACGT]ACC
EcoRI 254 GAATTC G^AATTC
EcoT22I 4869 ATGCAT ATGCA^T
FbaI 344 TGATCA T^GATCA
FseI 3754 GGCCGGCC GGCCGG^CC
FspI 4118 TGCGCA TGC^GCA
KpnI 2660 GGTACC GGTAC^C
KspI 2582 CCGCGG CCGC^GG
MamI 2805 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1464 TCCGGA T^CCGGA
NsiI 4869 ATGCAT ATGCA^T
PaeR7I 3198 CTCGAG C^TCGAG
PshAI 979 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4224 CGATCG CGAT^CG
RcaI 3428 TCATGA T^CATGA
SacII 2582 CCGCGG CCGC^GG
SalI 5505 GTCGAC G^TCGAC
SapI 423 GAAGAGC GCTCTTC(1/4)
SmaI 2133 CCCGGG CCC^GGG
SpeI 4814 ACTAGT A^CTAGT
XhoI 3198 CTCGAG C^TCGAG
XmaCI 2133 CCCGGG C^CCGGG
XmaI 2133 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1471 CCTGAGG CC^T[ACGT]AGG
5538 CCTAAGG
Aor51HI 76 AGCGCT AGC^GCT
2666 AGCGCT
AvaI 2133 CCCGGG C^[CT]CG[GA]G
3198 CTCGAG
AvrII 1734 CCTAGG C^CTAGG
4534 CCTAGG
BbeI 2906 GGCGCC GGCGC^C
4723 GGCGCC
BbvCI 2591 CCTCAGC CCTCAGC(-5/-2)
2370 GCTGAGG
BlnI 1734 CCTAGG C^CTAGG
4534 CCTAGG
BsmBI 1797 CGTCTC CGTCTC(1/5)
3495 CGTCTC
BsoBI 2133 CCCGGG C^[CT]CG[GA]G
3198 CTCGAG
BssHII 3687 GCGCGC G^CGCGC
3906 GCGCGC
Bsu36I 1471 CCTGAGG CC^T[ACGT]AGG
5538 CCTAAGG
EagI 3753 CGGCCG C^GGCCG
4609 CGGCCG
EclXI 3753 CGGCCG C^GGCCG
4609 CGGCCG
Eco47III 76 AGCGCT AGC^GCT
2666 AGCGCT
Eco52I 3753 CGGCCG C^GGCCG
4609 CGGCCG
Eco81I 1471 CCTGAGG CC^T[ACGT]AGG
5538 CCTAAGG
KasI 2906 GGCGCC G^GCGCC
4723 GGCGCC
NaeI 3755 GCCGGC GCC^GGC
4313 GCCGGC
NarI 2906 GGCGCC GG^CGCC
4723 GGCGCC
NgoMIV 3755 GCCGGC G^CCGGC
4313 GCCGGC
PstI 135 CTGCAG CTGCA^G
4009 CTGCAG
SacI 5063 GAGCTC GAGCT^C
5671 GAGCTC
SfoI 2906 GGCGCC GGC^GCC
4723 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AspI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII