##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AscI        
AseI        AsnI        Asp700I     BciVI       BfrI        
BglII       BsiWI       BspDI       BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DrdI        
EcoNI       EcoRV       HindIII     HpaI        MfeI        
MluI        MunI        NdeI        NheI        NotI        
NruI        NspV        PacI        PinAI       PmeI        
PshBI       Psp1406I    RsrII       SbfI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       Sse8387I    
SspI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       2660  GGTACC               G^GTACC
AccIII       1464  TCCGGA               T^CCGGA
AcsI          254  GAATTC               [GA]^AATT[CT]
AhdI         2122  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          254  GAATTC               [GA]^AATT[CT]
Asp718I      2660  GGTACC               G^GTACC
AspEI        2122  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        4118  TGCGCA               TGC^GCA
BamHI         777  GGATCC               G^GATCC
BcgI         2746  GCAGCCCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          344  TGATCA               T^GATCA
BsaBI        2805  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1464  TCCGGA               T^CCGGA
BspEI        1464  TCCGGA               T^CCGGA
BspHI        3428  TCATGA               T^CATGA
BspLU11I     4859  ACATGT               A^CATGT
BsrDI         396  CATTGC               GCAATG(2/0)
BstEII       3393  GGTCACC              G^GT[ACGT]ACC
BstPI        3393  GGTCACC              G^GT[ACGT]ACC
DraI         5309  TTTAAA               TTT^AAA
DraIII        520  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2122  GACCTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3393  GGTCACC              G^GT[ACGT]ACC
EcoRI         254  GAATTC               G^AATTC
EcoT22I      4869  ATGCAT               ATGCA^T
FbaI          344  TGATCA               T^GATCA
FseI         3754  GGCCGGCC             GGCCGG^CC
FspI         4118  TGCGCA               TGC^GCA
KpnI         2660  GGTACC               GGTAC^C
KspI         2582  CCGCGG               CCGC^GG
MamI         2805  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1464  TCCGGA               T^CCGGA
NsiI         4869  ATGCAT               ATGCA^T
PaeR7I       3198  CTCGAG               C^TCGAG
PshAI         979  GACACAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         4224  CGATCG               CGAT^CG
RcaI         3428  TCATGA               T^CATGA
SacII        2582  CCGCGG               CCGC^GG
SalI         5505  GTCGAC               G^TCGAC
SapI          423  GAAGAGC              GCTCTTC(1/4)
SmaI         2133  CCCGGG               CCC^GGG
SpeI         4814  ACTAGT               A^CTAGT
XhoI         3198  CTCGAG               C^TCGAG
XmaCI        2133  CCCGGG               C^CCGGG
XmaI         2133  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI         1471  CCTGAGG              CC^T[ACGT]AGG
             5538  CCTAAGG             
Aor51HI        76  AGCGCT               AGC^GCT
             2666  AGCGCT              
AvaI         2133  CCCGGG               C^[CT]CG[GA]G
             3198  CTCGAG              
AvrII        1734  CCTAGG               C^CTAGG
             4534  CCTAGG              
BbeI         2906  GGCGCC               GGCGC^C
             4723  GGCGCC              
BbvCI        2591  CCTCAGC              CCTCAGC(-5/-2)
             2370  GCTGAGG             
BlnI         1734  CCTAGG               C^CTAGG
             4534  CCTAGG              
BsmBI        1797  CGTCTC               CGTCTC(1/5)
             3495  CGTCTC              
BsoBI        2133  CCCGGG               C^[CT]CG[GA]G
             3198  CTCGAG              
BssHII       3687  GCGCGC               G^CGCGC
             3906  GCGCGC              
Bsu36I       1471  CCTGAGG              CC^T[ACGT]AGG
             5538  CCTAAGG             
EagI         3753  CGGCCG               C^GGCCG
             4609  CGGCCG              
EclXI        3753  CGGCCG               C^GGCCG
             4609  CGGCCG              
Eco47III       76  AGCGCT               AGC^GCT
             2666  AGCGCT              
Eco52I       3753  CGGCCG               C^GGCCG
             4609  CGGCCG              
Eco81I       1471  CCTGAGG              CC^T[ACGT]AGG
             5538  CCTAAGG             
KasI         2906  GGCGCC               G^GCGCC
             4723  GGCGCC              
NaeI         3755  GCCGGC               GCC^GGC
             4313  GCCGGC              
NarI         2906  GGCGCC               GG^CGCC
             4723  GGCGCC              
NgoMIV       3755  GCCGGC               G^CCGGC
             4313  GCCGGC              
PstI          135  CTGCAG               CTGCA^G
             4009  CTGCAG              
SacI         5063  GAGCTC               GAGCT^C
             5671  GAGCTC              
SfoI         2906  GGCGCC               GGC^GCC
             4723  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       AspHI       AspI        AvaII       
BalI        BanI        BanII       BbrPI       BbsI        
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PmaCI       PmlI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfiI        SmlI        
SphI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XcmI        
XhoII