################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccI AclI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BglI BlnI BlpI Bpu1102I BsiWI BsoBI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I CelII CpoI DraIII Eam1105I Eco47III Eco81I EcoO65I FseI FspI HaeII KasI KspI MluI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 4104 GGTACC G^GTACC AccIII 1589 TCCGGA T^CCGGA AcyI 41 GACGCC G[GA]^CG[CT]C AgeI 1300 ACCGGT A^CCGGT Asp718I 4104 GGTACC G^GTACC BalI 761 TGGCCA TGG^CCA BciVI 3056 GGATAC GTATCC(6/5) BsaBI 4670 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 41 GACGCC G[GA]^CG[CT]C BsaI 4543 GGTCTC GGTCTC(1/5) BseAI 1589 TCCGGA T^CCGGA BsgI 435 GTGCAG GTGCAG(16/14) BsiEI 116 CGGCCG CG[GA][CT]^CG BsmBI 2220 CGTCTC CGTCTC(1/5) BspDI 3608 ATCGAT AT^CGAT BspEI 1589 TCCGGA T^CCGGA BspMI 3192 ACCTGC ACCTGC(4/8) BsrBI 5440 GAGCGG CCGCTC(-3/-3) BssSI 769 CACGAG CACGAG(-5/-1) BstAPI 1459 GCAACCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1153 TTCGAA TT^CGAA CfoI 1160 GCGC GCG^C ClaI 3608 ATCGAT AT^CGAT EagI 116 CGGCCG C^GGCCG EclXI 116 CGGCCG C^GGCCG Eco52I 116 CGGCCG C^GGCCG EcoNI 1752 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 399 GAATTC G^AATTC HhaI 1160 GCGC GCG^C Hin1I 41 GACGCC G[GA]^CG[CT]C HinP1I 1160 GCGC G^CGC HindIII 4041 AAGCTT A^AGCTT HpaI 4966 GTTAAC GTT^AAC KpnI 4104 GGTACC GGTAC^C MamI 4670 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3457 CAATTG C^AATTG MluNI 761 TGGCCA TGG^CCA MroI 1589 TCCGGA T^CCGGA MscI 761 TGGCCA TGG^CCA MunI 3457 CAATTG C^AATTG NcoI 743 CCATGG C^CATGG NheI 425 GCTAGC G^CTAGC NspV 1153 TTCGAA TT^CGAA PinAI 1300 ACCGGT A^CCGGT PshAI 1413 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 1936 ACCAGGT A^CC[AT]GGT SfuI 1153 TTCGAA TT^CGAA XcmI 1216 CCAGCCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 330 CGCG CG^CG 1379 CGCG AflII 3524 CTTAAG C^TTAAG 5446 CTTAAG AlwNI 4392 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 4452 CAGTTTCTG BclI 1093 TGATCA T^GATCA 4777 TGATCA BcnI 409 CCGGG CC^[GC]GG 5743 CCGGG BfrI 3524 CTTAAG C^TTAAG 5446 CTTAAG BglII 306 AGATCT A^GATCT 3258 AGATCT BsaAI 1583 TACGTG [CT]AC^GT[GA] 4979 CACGTA Bsp1407I 3490 TGTACA T^GTACA 3962 TGTACA BspLU11I 3487 ACATGT A^CATGT 3965 ACATGT BsrFI 553 ACCGGC [GA]^CCGG[CT] 1300 ACCGGT BsrGI 3490 TGTACA T^GTACA 3962 TGTACA BstUI 330 CGCG CG^CG 1379 CGCG Cfr10I 553 ACCGGC [GA]^CCGG[CT] 1300 ACCGGT DsaI 743 CCATGG C^C[GA][CT]GG 1445 CCACGG EaeI 116 CGGCCG [CT]^GGCC[GA] 761 TGGCCA EcoRV 4844 GATATC GAT^ATC 4927 GATATC FauI 4825 CCCGC CCCGC(4/6) 2229 GCGGG FbaI 1093 TGATCA T^GATCA 4777 TGATCA HgaI 41 GACGC GACGC(5/10) 2101 GCGTC MvnI 330 CGCG CG^CG 1379 CGCG NciI 409 CCGGG CC^[GC]GG 5743 CCGGG NdeI 966 CATATG CA^TATG 4644 CATATG PflMI 136 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1003 CCACGAACTGG PvuII 1282 CAGCTG CAG^CTG 5621 CAGCTG SspBI 3490 TGTACA T^GTACA 3962 TGTACA StuI 1826 AGGCCT AGG^CCT 1963 AGGCCT Van91I 136 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1003 CCACGAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BamHI BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DrdI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PshBI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI