##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AclI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BcgI BglI
BlnI BlpI Bpu1102I BsiWI BsoBI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraIII Eam1105I
Eco47III Eco81I EcoO65I FseI FspI
HaeII KasI KspI MluI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflFI PmaCI PmeI PmlI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 4104 GGTACC G^GTACC
AccIII 1589 TCCGGA T^CCGGA
AcyI 41 GACGCC G[GA]^CG[CT]C
AgeI 1300 ACCGGT A^CCGGT
Asp718I 4104 GGTACC G^GTACC
BalI 761 TGGCCA TGG^CCA
BciVI 3056 GGATAC GTATCC(6/5)
BsaBI 4670 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 41 GACGCC G[GA]^CG[CT]C
BsaI 4543 GGTCTC GGTCTC(1/5)
BseAI 1589 TCCGGA T^CCGGA
BsgI 435 GTGCAG GTGCAG(16/14)
BsiEI 116 CGGCCG CG[GA][CT]^CG
BsmBI 2220 CGTCTC CGTCTC(1/5)
BspDI 3608 ATCGAT AT^CGAT
BspEI 1589 TCCGGA T^CCGGA
BspMI 3192 ACCTGC ACCTGC(4/8)
BsrBI 5440 GAGCGG CCGCTC(-3/-3)
BssSI 769 CACGAG CACGAG(-5/-1)
BstAPI 1459 GCAACCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1153 TTCGAA TT^CGAA
CfoI 1160 GCGC GCG^C
ClaI 3608 ATCGAT AT^CGAT
EagI 116 CGGCCG C^GGCCG
EclXI 116 CGGCCG C^GGCCG
Eco52I 116 CGGCCG C^GGCCG
EcoNI 1752 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 399 GAATTC G^AATTC
HhaI 1160 GCGC GCG^C
Hin1I 41 GACGCC G[GA]^CG[CT]C
HinP1I 1160 GCGC G^CGC
HindIII 4041 AAGCTT A^AGCTT
HpaI 4966 GTTAAC GTT^AAC
KpnI 4104 GGTACC GGTAC^C
MamI 4670 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3457 CAATTG C^AATTG
MluNI 761 TGGCCA TGG^CCA
MroI 1589 TCCGGA T^CCGGA
MscI 761 TGGCCA TGG^CCA
MunI 3457 CAATTG C^AATTG
NcoI 743 CCATGG C^CATGG
NheI 425 GCTAGC G^CTAGC
NspV 1153 TTCGAA TT^CGAA
PinAI 1300 ACCGGT A^CCGGT
PshAI 1413 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1936 ACCAGGT A^CC[AT]GGT
SfuI 1153 TTCGAA TT^CGAA
XcmI 1216 CCAGCCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 330 CGCG CG^CG
1379 CGCG
AflII 3524 CTTAAG C^TTAAG
5446 CTTAAG
AlwNI 4392 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
4452 CAGTTTCTG
BclI 1093 TGATCA T^GATCA
4777 TGATCA
BcnI 409 CCGGG CC^[GC]GG
5743 CCGGG
BfrI 3524 CTTAAG C^TTAAG
5446 CTTAAG
BglII 306 AGATCT A^GATCT
3258 AGATCT
BsaAI 1583 TACGTG [CT]AC^GT[GA]
4979 CACGTA
Bsp1407I 3490 TGTACA T^GTACA
3962 TGTACA
BspLU11I 3487 ACATGT A^CATGT
3965 ACATGT
BsrFI 553 ACCGGC [GA]^CCGG[CT]
1300 ACCGGT
BsrGI 3490 TGTACA T^GTACA
3962 TGTACA
BstUI 330 CGCG CG^CG
1379 CGCG
Cfr10I 553 ACCGGC [GA]^CCGG[CT]
1300 ACCGGT
DsaI 743 CCATGG C^C[GA][CT]GG
1445 CCACGG
EaeI 116 CGGCCG [CT]^GGCC[GA]
761 TGGCCA
EcoRV 4844 GATATC GAT^ATC
4927 GATATC
FauI 4825 CCCGC CCCGC(4/6)
2229 GCGGG
FbaI 1093 TGATCA T^GATCA
4777 TGATCA
HgaI 41 GACGC GACGC(5/10)
2101 GCGTC
MvnI 330 CGCG CG^CG
1379 CGCG
NciI 409 CCGGG CC^[GC]GG
5743 CCGGG
NdeI 966 CATATG CA^TATG
4644 CATATG
PflMI 136 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1003 CCACGAACTGG
PvuII 1282 CAGCTG CAG^CTG
5621 CAGCTG
SspBI 3490 TGTACA T^GTACA
3962 TGTACA
StuI 1826 AGGCCT AGG^CCT
1963 AGGCCT
Van91I 136 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1003 CCACGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BamHI BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DrdI EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PshBI RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI