##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AclI AgeI AscI AseI AsnI
AviII BbeI BcgI BclI BsiWI
BssHII BstAPI BstBI CpoI DrdI
EcoRI EcoRV FbaI FseI FspI
HpaI KasI KspI MluI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 2750 GACGTC GACGT^C
Acc65I 1822 GGTACC G^GTACC
AcyI 2750 GACGTC G[GA]^CG[CT]C
AhdI 178 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1408 AGCGCT AGC^GCT
Asp700I 1901 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1822 GGTACC G^GTACC
AspEI 178 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2082 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1891 CACGTG CAC^GTG
BglI 1099 GCCCCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 689 AGATCT A^GATCT
BsaBI 3903 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2750 GACGTC G[GA]^CG[CT]C
BsiEI 2334 CGGCCG CG[GA][CT]^CG
BsmI 4968 GCATTC GAATGC(1/-1)
BspDI 2470 ATCGAT AT^CGAT
BspHI 3853 TCATGA T^CATGA
BsrDI 4240 GCAATG GCAATG(2/0)
BsrFI 1355 GCCGGT [GA]^CCGG[CT]
Bst1107I 4948 GTATAC GTA^TAC
BstZ17I 4948 GTATAC GTA^TAC
Cfr10I 1355 GCCGGT [GA]^CCGG[CT]
ClaI 2470 ATCGAT AT^CGAT
EagI 2334 CGGCCG C^GGCCG
Eam1105I 178 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2334 CGGCCG C^GGCCG
Eco47III 1408 AGCGCT AGC^GCT
Eco52I 2334 CGGCCG C^GGCCG
EcoT22I 5143 ATGCAT ATGCA^T
Hin1I 2750 GACGTC G[GA]^CG[CT]C
HincII 3082 GTTGAC GT[CT]^[GA]AC
HindII 3082 GTTGAC GT[CT]^[GA]AC
KpnI 1822 GGTACC GGTAC^C
MamI 3903 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3228 CAATTG C^AATTG
MunI 3228 CAATTG C^AATTG
NheI 3323 GCTAGC G^CTAGC
NsiI 5143 ATGCAT ATGCA^T
PflFI 2082 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1891 CACGTG CAC^GTG
PmlI 1891 CACGTG CAC^GTG
RcaI 3853 TCATGA T^CATGA
SbfI 925 CCTGCAGG CCTGCA^GG
ScaI 5607 AGTACT AGT^ACT
SexAI 2844 ACCTGGT A^CC[AT]GGT
Sse8387I 925 CCTGCAGG CCTGCA^GG
Tth111I 2082 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1901 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1476 GTCTAC GT^[AC][GT]AC
4948 GTATAC
AccIII 3296 TCCGGA T^CCGGA
4455 TCCGGA
AflII 2713 CTTAAG C^TTAAG
2745 CTTAAG
Alw44I 621 GTGCAC G^TGCAC
3663 GTGCAC
AocI 807 CCTCAGG CC^T[ACGT]AGG
3195 CCTTAGG
ApaLI 621 GTGCAC G^TGCAC
3663 GTGCAC
BamHI 1445 GGATCC G^GATCC
3057 GGATCC
BciVI 1054 GGATAC GTATCC(6/5)
1296 GGATAC
BfrI 2713 CTTAAG C^TTAAG
2745 CTTAAG
BlpI 46 GCTGAGC GC^T[ACGT]AGC
4685 GCTGAGC
Bpu1102I 46 GCTGAGC GC^T[ACGT]AGC
4685 GCTGAGC
BsaAI 1891 CACGTG [CT]AC^GT[GA]
2672 CACGTA
BseAI 3296 TCCGGA T^CCGGA
4455 TCCGGA
BspEI 3296 TCCGGA T^CCGGA
4455 TCCGGA
BspLU11I 1382 ACATGT A^CATGT
5082 ACATGT
BspMI 4737 ACCTGC ACCTGC(4/8)
3203 GCAGGT
BsrBI 4629 CCGCTC CCGCTC(-3/-3)
5122 GAGCGG
Bsu36I 807 CCTCAGG CC^T[ACGT]AGG
3195 CCTTAGG
CelII 46 GCTGAGC GC^T[ACGT]AGC
4685 GCTGAGC
DraI 2908 TTTAAA TTT^AAA
4118 TTTAAA
DraIII 1910 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3565 CACACAGTG
EarI 2110 GAAGAG CTCTTC(1/4)
2631 GAAGAG
Eco81I 807 CCTCAGG CC^T[ACGT]AGG
3195 CCTTAGG
EcoNI 2192 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4352 CCTGGGAGAGG
HindIII 3412 AAGCTT A^AGCTT
5514 AAGCTT
Ksp632I 2110 GAAGAG CTCTTC(1/4)
2631 GAAGAG
MroI 3296 TCCGGA T^CCGGA
4455 TCCGGA
PpuMI 325 GGGACCC [GA]G^G[AT]CC[CT]
930 AGGACCT
SphI 3154 GCATGC GCATG^C
5141 GCATGC
XbaI 1560 TCTAGA T^CTAGA
5653 TCTAGA
XcmI 1689 CCACTGTTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2354 CCACACGAAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlnI BmyI BpmI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII