##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BfrI BglI BlnI BsaBI
BsiEI BsiWI BspDI BsrFI Bst1107I
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoT22I FseI FspI
KpnI MamI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SspI StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 3165 TCCGGA T^CCGGA
Alw44I 3023 GTGCAC G^TGCAC
AlwNI 4192 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1934 GGGCCC GGGCC^C
ApaLI 3023 GTGCAC G^TGCAC
AspI 5478 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3827 CACGTG CAC^GTG
BclI 4188 TGATCA T^GATCA
BsaAI 3827 CACGTG [CT]AC^GT[GA]
BseAI 3165 TCCGGA T^CCGGA
BspEI 3165 TCCGGA T^CCGGA
BspHI 123 TCATGA T^CATGA
BsrBI 1067 CCGCTC CCGCTC(-3/-3)
BssHII 870 GCGCGC G^CGCGC
BstEII 6044 GGTCACC G^GT[ACGT]ACC
BstPI 6044 GGTCACC G^GT[ACGT]ACC
DrdI 6036 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 6044 GGTCACC G^GT[ACGT]ACC
EcoRI 45 GAATTC G^AATTC
EcoRV 3618 GATATC GAT^ATC
FbaI 4188 TGATCA T^GATCA
HindIII 2805 AAGCTT A^AGCTT
HpaI 131 GTTAAC GTT^AAC
KspI 217 CCGCGG CCGC^GG
MfeI 4453 CAATTG C^AATTG
MluI 4086 ACGCGT A^CGCGT
MroI 3165 TCCGGA T^CCGGA
MunI 4453 CAATTG C^AATTG
NcoI 3784 CCATGG C^CATGG
PflFI 5478 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3827 CACGTG CAC^GTG
PmlI 3827 CACGTG CAC^GTG
RcaI 123 TCATGA T^CATGA
SacII 217 CCGCGG CCGC^GG
SbfI 484 CCTGCAGG CCTGCA^GG
SexAI 4020 ACCTGGT A^CC[AT]GGT
SpeI 3526 ACTAGT A^CTAGT
SphI 5090 GCATGC GCATG^C
Sse8387I 484 CCTGCAGG CCTGCA^GG
Tth111I 5478 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 22 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BamHI 1757 GGATCC G^GATCC
3996 GGATCC
BbeI 2134 GGCGCC GGCGC^C
3005 GGCGCC
BcgI 1278 CGAGCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5763 CGAGTGTCATGC
BciVI 2946 GGATAC GTATCC(6/5)
4583 GGATAC
BglII 19 AGATCT A^GATCT
27 AGATCT
BsaWI 1210 ACCGGA [AT]^CCGG[AT]
3165 TCCGGA
BsmBI 81 CGTCTC CGTCTC(1/5)
5286 GAGACG
Bsp1407I 1926 TGTACA T^GTACA
4951 TGTACA
BspLU11I 1557 ACATGT A^CATGT
3687 ACATGT
BsrGI 1926 TGTACA T^GTACA
4951 TGTACA
BssSI 1250 CACGAG CACGAG(-5/-1)
3650 CTCGTG
DraIII 4965 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5148 CACCTAGTG
EcoNI 637 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5080 CCTGCTGCAGG
HgaI 1145 GACGC GACGC(5/10)
80 GCGTC
KasI 2134 GGCGCC G^GCGCC
3005 GGCGCC
NarI 2134 GGCGCC GG^CGCC
3005 GGCGCC
NheI 2355 GCTAGC G^CTAGC
4960 GCTAGC
SfoI 2134 GGCGCC GGC^GCC
3005 GGCGCC
SspBI 1926 TGTACA T^GTACA
4951 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI