##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AscI AseI AsnI Asp718I AspI
BamHI BbrPI BciVI BclI BsaBI
BsiWI BsmBI BspDI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I ClaI CpoI DsaI
EagI EclXI Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI HgaI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2842 GTCTAC GT^[AC][GT]AC
AccIII 1162 TCCGGA T^CCGGA
AcyI 3914 GGCGCC G[GA]^CG[CT]C
AflII 3976 CTTAAG C^TTAAG
AhdI 3831 GACTTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2986 GTGCAC G^TGCAC
ApaLI 2986 GTGCAC G^TGCAC
AspEI 3831 GACTTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 698 CCTAGG C^CTAGG
BbeI 3914 GGCGCC GGCGC^C
BcgI 773 GCAGATGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3976 CTTAAG C^TTAAG
BglII 407 AGATCT A^GATCT
BlnI 698 CCTAGG C^CTAGG
BlpI 3024 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3024 GCTCAGC GC^T[ACGT]AGC
BsaAI 4388 CACGTA [CT]AC^GT[GA]
BsaHI 3914 GGCGCC G[GA]^CG[CT]C
BsaWI 1162 TCCGGA [AT]^CCGG[AT]
BseAI 1162 TCCGGA T^CCGGA
BsiEI 667 CGATCG CG[GA][CT]^CG
BsmI 1007 GCATTC GAATGC(1/-1)
BspEI 1162 TCCGGA T^CCGGA
BsrBI 2766 CCGCTC CCGCTC(-3/-3)
BsrFI 3871 GCCGGT [GA]^CCGG[CT]
CelII 3024 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3871 GCCGGT [GA]^CCGG[CT]
Eam1105I 3831 GACTTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2318 GATATC GAT^ATC
Hin1I 3914 GGCGCC G[GA]^CG[CT]C
KasI 3914 GGCGCC G^GCGCC
MroI 1162 TCCGGA T^CCGGA
NarI 3914 GGCGCC GG^CGCC
PshAI 1820 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 667 CGATCG CGAT^CG
SapI 1396 GAAGAGC GCTCTTC(1/4)
SexAI 4093 ACCTGGT A^CC[AT]GGT
SfoI 3914 GGCGCC GGC^GCC
SmaI 271 CCCGGG CCC^GGG
XmaCI 271 CCCGGG C^CCGGG
XmaI 271 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 634 AGCGCT AGC^GCT
721 AGCGCT
AviII 2563 TGCGCA TGC^GCA
4337 TGCGCA
BglI 1251 GCCTTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2539 GCCCTGCTGGC
Bsp1407I 1312 TGTACA T^GTACA
1846 TGTACA
BspHI 2489 TCATGA T^CATGA
4259 TCATGA
BsrGI 1312 TGTACA T^GTACA
1846 TGTACA
BssSI 3051 CACGAG CACGAG(-5/-1)
4236 CTCGTG
BstAPI 1779 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3250 GCAGACCCTGC
DraI 3707 TTTAAA TTT^AAA
4453 TTTAAA
DraIII 1509 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1797 CACAGGGTG
DrdI 1152 GACAGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3532 GACACAGCTGTC
Eco47III 634 AGCGCT AGC^GCT
721 AGCGCT
EcoNI 824 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1032 CCTGCTGCAGG
FspI 2563 TGCGCA TGC^GCA
4337 TGCGCA
MaeII 3445 ACGT A^CGT
4389 ACGT
MslI 2789 CATGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3738 CATAAAAGTG
PpuMI 487 AGGACCC [GA]G^G[AT]CC[CT]
3158 AGGTCCC
PvuII 807 CAGCTG CAG^CTG
3536 CAGCTG
RcaI 2489 TCATGA T^CATGA
4259 TCATGA
ScaI 187 AGTACT AGT^ACT
4286 AGTACT
SspBI 1312 TGTACA T^GTACA
1846 TGTACA
SspI 426 AATATT AAT^ATT
4413 AATATT
StuI 1207 AGGCCT AGG^CCT
2681 AGGCCT
XcmI 1477 CCAAGCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1690 CCATGAGCCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI