##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AgeI ApaI
AscI Asp718I BamHI BbeI BbvCI
BglI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII BssSI BstAPI
CelII ClaI CpoI DraIII DrdI
EcoRI EcoRV FseI HpaI KasI
KpnI MamI MluI MroI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PinAI PvuI RcaI RsrII
SbfI SexAI SfiI SfoI SgrAI
SpeI Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccII 30 CGCG CG^CG
AclI 5220 AACGTT AA^CGTT
AcyI 657 GACGCC G[GA]^CG[CT]C
AhdI 4245 GACAAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1018 GGATC GGATC(4/5)
AlwNI 3235 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 875 CCTCAGG CC^T[ACGT]AGG
Aor51HI 805 AGCGCT AGC^GCT
Asp700I 4319 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4245 GACAAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 810 TGCGCA TGC^GCA
AvrII 6265 CCTAGG C^CTAGG
BalI 3776 TGGCCA TGG^CCA
BbrPI 3553 CACGTG CAC^GTG
BcgI 2329 GCAGAGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 189 TGATCA T^GATCA
BlnI 6265 CCTAGG C^CTAGG
BsaHI 657 GACGCC G[GA]^CG[CT]C
BsgI 2121 CTGCAC GTGCAG(16/14)
BsiEI 909 CGGCCG CG[GA][CT]^CG
BspMI 4139 ACCTGC ACCTGC(4/8)
BsrFI 348 ACCGGC [GA]^CCGG[CT]
BstEII 1083 GGTCACC G^GT[ACGT]ACC
BstPI 1083 GGTCACC G^GT[ACGT]ACC
BstUI 30 CGCG CG^CG
Bsu36I 875 CCTCAGG CC^T[ACGT]AGG
Cfr10I 348 ACCGGC [GA]^CCGG[CT]
EagI 909 CGGCCG C^GGCCG
Eam1105I 4245 GACAAATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 909 CGGCCG C^GGCCG
Eco47III 805 AGCGCT AGC^GCT
Eco52I 909 CGGCCG C^GGCCG
Eco81I 875 CCTCAGG CC^T[ACGT]AGG
EcoNI 9 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1083 GGTCACC G^GT[ACGT]ACC
EcoT22I 1257 ATGCAT ATGCA^T
FbaI 189 TGATCA T^GATCA
FspI 810 TGCGCA TGC^GCA
HaeII 805 AGCGCT [GA]GCGC^[CT]
HgaI 657 GACGC GACGC(5/10)
Hin1I 657 GACGCC G[GA]^CG[CT]C
HincII 1522 GTCGAC GT[CT]^[GA]AC
HindII 1522 GTCGAC GT[CT]^[GA]AC
KspI 29 CCGCGG CCGC^GG
MfeI 5983 CAATTG C^AATTG
MluNI 3776 TGGCCA TGG^CCA
MscI 3776 TGGCCA TGG^CCA
MunI 5983 CAATTG C^AATTG
MvnI 30 CGCG CG^CG
NheI 1950 GCTAGC G^CTAGC
NsiI 1257 ATGCAT ATGCA^T
PmaCI 3553 CACGTG CAC^GTG
PmeI 3319 GTTTAAAC GTTT^AAAC
PmlI 3553 CACGTG CAC^GTG
Psp1406I 5220 AACGTT AA^CGTT
PstI 4141 CTGCAG CTGCA^G
SacII 29 CCGCGG CCGC^GG
SalI 1522 GTCGAC G^TCGAC
ScaI 1594 AGTACT AGT^ACT
SmaI 1068 CCCGGG CCC^GGG
SnaBI 5972 TACGTA TAC^GTA
StuI 676 AGGCCT AGG^CCT
XmaCI 1068 CCCGGG C^CCGGG
XmaI 1068 CCCGGG C^CCGGG
XmnI 4319 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 2918 CTTAAG C^TTAAG
3311 CTTAAG
Alw44I 3550 GTGCAC G^TGCAC
5899 GTGCAC
ApaLI 3550 GTGCAC G^TGCAC
5899 GTGCAC
AspI 241 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4080 GACTCTGTC
AvaI 1068 CCCGGG C^[CT]CG[GA]G
1608 CCCGAG
BanI 228 GGTGCC G^G[CT][GA]CC
3519 GGCACC
BcnI 1068 CCCGG CC^[GC]GG
1069 CCGGG
BfrI 2918 CTTAAG C^TTAAG
3311 CTTAAG
BsaI 1277 GAGACC GGTCTC(1/5)
2250 GAGACC
BsoBI 1068 CCCGGG C^[CT]CG[GA]G
1608 CCCGAG
Bst1107I 2422 GTATAC GTA^TAC
5311 GTATAC
BstBI 959 TTCGAA TT^CGAA
3633 TTCGAA
BstZ17I 2422 GTATAC GTA^TAC
5311 GTATAC
CfoI 806 GCGC GCG^C
811 GCGC
FauI 28 CCCGC CCCGC(4/6)
297 CCCGC
HhaI 806 GCGC GCG^C
811 GCGC
HinP1I 806 GCGC G^CGC
811 GCGC
NciI 1068 CCCGG CC^[GC]GG
1069 CCGGG
NspV 959 TTCGAA TT^CGAA
3633 TTCGAA
PflFI 241 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4080 GACTCTGTC
PflMI 478 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4733 CCAGAATTTGG
PshAI 752 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2042 GACCCCAGTC
PvuII 1392 CAGCTG CAG^CTG
2306 CAGCTG
SacI 856 GAGCTC GAGCT^C
1494 GAGCTC
SfuI 959 TTCGAA TT^CGAA
3633 TTCGAA
SwaI 1923 ATTTAAAT ATTT^AAAT
2794 ATTTAAAT
Tth111I 241 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4080 GACTCTGTC
Van91I 478 CCATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4733 CCAGAATTTGG
XbaI 1030 TCTAGA T^CTAGA
4689 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI ApoI AseI AsnI AspHI
AvaII BanII BbsI BbvI BciVI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII