##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AccIII Aor51HI AscI AseI AsnI
BamHI BbvCI BciVI BseAI BsiEI
BspDI BspEI BsrBI BstBI ClaI
CpoI EagI EclXI Eco47III Eco52I
KspI MfeI MluI MroI MunI
NotI NruI NspV PacI PmeI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1245 GACGTC GACGT^C
AclI 3545 AACGTT AA^CGTT
AflII 5739 CTTAAG C^TTAAG
AgeI 2720 ACCGGT A^CCGGT
AhdI 2719 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4195 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2719 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1997 TGCGCA TGC^GCA
BbrPI 4765 CACGTG CAC^GTG
BfrI 5739 CTTAAG C^TTAAG
BglII 1105 AGATCT A^GATCT
BsaAI 4765 CACGTG [CT]AC^GT[GA]
BsaBI 5239 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2720 ACCGGT [AT]^CCGG[AT]
BssHII 2246 GCGCGC G^CGCGC
Bst1107I 2216 GTATAC GTA^TAC
BstZ17I 2216 GTATAC GTA^TAC
DraI 547 TTTAAA TTT^AAA
DrdI 2996 GACCTATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2719 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1291 GAATTC G^AATTC
FseI 2804 GGCCGGCC GGCCGG^CC
FspI 1997 TGCGCA TGC^GCA
HpaI 3670 GTTAAC GTT^AAC
MamI 5239 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 4948 CATATG CA^TATG
PinAI 2720 ACCGGT A^CCGGT
PmaCI 4765 CACGTG CAC^GTG
PmlI 4765 CACGTG CAC^GTG
PshAI 5414 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3545 AACGTT AA^CGTT
SacI 1013 GAGCTC GAGCT^C
SapI 1219 GAAGAGC GCTCTTC(1/4)
SmaI 765 CCCGGG CCC^GGG
SpeI 1087 ACTAGT A^CTAGT
SphI 5770 GCATGC GCATG^C
SspI 4798 AATATT AAT^ATT
XbaI 601 TCTAGA T^CTAGA
XcmI 4417 CCAAGTGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 765 CCCGGG C^CCGGG
XmaI 765 CCCGGG C^CCGGG
XmnI 4195 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1720 GGTACC G^GTACC
3125 GGTACC
Alw44I 2177 GTGCAC G^TGCAC
3515 GTGCAC
AlwI 891 GATCC GGATC(4/5)
1918 GATCC
ApaLI 2177 GTGCAC G^TGCAC
3515 GTGCAC
Asp718I 1720 GGTACC G^GTACC
3125 GGTACC
AvrII 1231 CCTAGG C^CTAGG
2955 CCTAGG
BbeI 101 GGCGCC GGCGC^C
227 GGCGCC
BcgI 409 CGAGGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1427 GCACTGTCCTCG
BclI 952 TGATCA T^GATCA
2823 TGATCA
BglI 150 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
419 GCCGAGTTGGC
BlnI 1231 CCTAGG C^CTAGG
2955 CCTAGG
BsaI 87 GGTCTC GGTCTC(1/5)
1546 GGTCTC
BsmBI 1390 GAGACG CGTCTC(1/5)
2142 GAGACG
BsrDI 922 CATTGC GCAATG(2/0)
1629 GCAATG
BstEII 170 GGTGACC G^GT[ACGT]ACC
292 GGTCACC
BstPI 170 GGTGACC G^GT[ACGT]ACC
292 GGTCACC
BstYI 1105 AGATCT [GA]^GATC[CT]
1917 AGATCC
DraIII 1971 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2100 CACAGAGTG
EcoO65I 170 GGTGACC G^GT[ACGT]ACC
292 GGTCACC
EcoRV 1598 GATATC GAT^ATC
4097 GATATC
EcoT22I 1695 ATGCAT ATGCA^T
5603 ATGCAT
FbaI 952 TGATCA T^GATCA
2823 TGATCA
HaeII 101 GGCGCC [GA]GCGC^[CT]
227 GGCGCC
HgaI 5274 GACGC GACGC(5/10)
35 GCGTC
HincII 1927 GTCAAC GT[CT]^[GA]AC
3670 GTTAAC
HindII 1927 GTCAAC GT[CT]^[GA]AC
3670 GTTAAC
HindIII 53 AAGCTT A^AGCTT
2354 AAGCTT
KasI 101 GGCGCC G^GCGCC
227 GGCGCC
KpnI 1720 GGTACC GGTAC^C
3125 GGTACC
MflI 1105 AGATCT [GA]^GATC[CT]
1917 AGATCC
NarI 101 GGCGCC GG^CGCC
227 GGCGCC
NheI 281 GCTAGC G^CTAGC
3388 GCTAGC
NsiI 1695 ATGCAT ATGCA^T
5603 ATGCAT
PaeR7I 1867 CTCGAG C^TCGAG
4641 CTCGAG
SfoI 101 GGCGCC GGC^GCC
227 GGCGCC
StuI 1712 AGGCCT AGG^CCT
3849 AGGCCT
XhoI 1867 CTCGAG C^TCGAG
4641 CTCGAG
XhoII 1105 AGATCT [GA]^GATC[CT]
1917 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrFI BsrGI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I