################################## # NO CUT ENZYMES ( 43 enzymes) # ################################## AccIII Aor51HI AscI AseI AsnI BamHI BbvCI BciVI BseAI BsiEI BspDI BspEI BsrBI BstBI ClaI CpoI EagI EclXI Eco47III Eco52I KspI MfeI MluI MroI MunI NotI NruI NspV PacI PmeI PshBI PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1245 GACGTC GACGT^C AclI 3545 AACGTT AA^CGTT AflII 5739 CTTAAG C^TTAAG AgeI 2720 ACCGGT A^CCGGT AhdI 2719 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 4195 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2719 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1997 TGCGCA TGC^GCA BbrPI 4765 CACGTG CAC^GTG BfrI 5739 CTTAAG C^TTAAG BglII 1105 AGATCT A^GATCT BsaAI 4765 CACGTG [CT]AC^GT[GA] BsaBI 5239 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2720 ACCGGT [AT]^CCGG[AT] BssHII 2246 GCGCGC G^CGCGC Bst1107I 2216 GTATAC GTA^TAC BstZ17I 2216 GTATAC GTA^TAC DraI 547 TTTAAA TTT^AAA DrdI 2996 GACCTATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2719 GACCGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1291 GAATTC G^AATTC FseI 2804 GGCCGGCC GGCCGG^CC FspI 1997 TGCGCA TGC^GCA HpaI 3670 GTTAAC GTT^AAC MamI 5239 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 4948 CATATG CA^TATG PinAI 2720 ACCGGT A^CCGGT PmaCI 4765 CACGTG CAC^GTG PmlI 4765 CACGTG CAC^GTG PshAI 5414 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3545 AACGTT AA^CGTT SacI 1013 GAGCTC GAGCT^C SapI 1219 GAAGAGC GCTCTTC(1/4) SmaI 765 CCCGGG CCC^GGG SpeI 1087 ACTAGT A^CTAGT SphI 5770 GCATGC GCATG^C SspI 4798 AATATT AAT^ATT XbaI 601 TCTAGA T^CTAGA XcmI 4417 CCAAGTGTCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 765 CCCGGG C^CCGGG XmaI 765 CCCGGG C^CCGGG XmnI 4195 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 1720 GGTACC G^GTACC 3125 GGTACC Alw44I 2177 GTGCAC G^TGCAC 3515 GTGCAC AlwI 891 GATCC GGATC(4/5) 1918 GATCC ApaLI 2177 GTGCAC G^TGCAC 3515 GTGCAC Asp718I 1720 GGTACC G^GTACC 3125 GGTACC AvrII 1231 CCTAGG C^CTAGG 2955 CCTAGG BbeI 101 GGCGCC GGCGC^C 227 GGCGCC BcgI 409 CGAGGAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1427 GCACTGTCCTCG BclI 952 TGATCA T^GATCA 2823 TGATCA BglI 150 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 419 GCCGAGTTGGC BlnI 1231 CCTAGG C^CTAGG 2955 CCTAGG BsaI 87 GGTCTC GGTCTC(1/5) 1546 GGTCTC BsmBI 1390 GAGACG CGTCTC(1/5) 2142 GAGACG BsrDI 922 CATTGC GCAATG(2/0) 1629 GCAATG BstEII 170 GGTGACC G^GT[ACGT]ACC 292 GGTCACC BstPI 170 GGTGACC G^GT[ACGT]ACC 292 GGTCACC BstYI 1105 AGATCT [GA]^GATC[CT] 1917 AGATCC DraIII 1971 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG 2100 CACAGAGTG EcoO65I 170 GGTGACC G^GT[ACGT]ACC 292 GGTCACC EcoRV 1598 GATATC GAT^ATC 4097 GATATC EcoT22I 1695 ATGCAT ATGCA^T 5603 ATGCAT FbaI 952 TGATCA T^GATCA 2823 TGATCA HaeII 101 GGCGCC [GA]GCGC^[CT] 227 GGCGCC HgaI 5274 GACGC GACGC(5/10) 35 GCGTC HincII 1927 GTCAAC GT[CT]^[GA]AC 3670 GTTAAC HindII 1927 GTCAAC GT[CT]^[GA]AC 3670 GTTAAC HindIII 53 AAGCTT A^AGCTT 2354 AAGCTT KasI 101 GGCGCC G^GCGCC 227 GGCGCC KpnI 1720 GGTACC GGTAC^C 3125 GGTACC MflI 1105 AGATCT [GA]^GATC[CT] 1917 AGATCC NarI 101 GGCGCC GG^CGCC 227 GGCGCC NheI 281 GCTAGC G^CTAGC 3388 GCTAGC NsiI 1695 ATGCAT ATGCA^T 5603 ATGCAT PaeR7I 1867 CTCGAG C^TCGAG 4641 CTCGAG SfoI 101 GGCGCC GGC^GCC 227 GGCGCC StuI 1712 AGGCCT AGG^CCT 3849 AGGCCT XhoI 1867 CTCGAG C^TCGAG 4641 CTCGAG XhoII 1105 AGATCT [GA]^GATC[CT] 1917 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiWI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I