##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AflIII AgeI Aor51HI
AscI AseI AsnI AviII BbeI
BbrPI BsiWI BspDI BspLU11I BssHII
BstBI ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI KasI KspI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 818 GTATAC GT^[AC][GT]AC
AccIII 381 TCCGGA T^CCGGA
AflII 5575 CTTAAG C^TTAAG
AhdI 5653 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5653 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 979 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4130 GGATCC G^GATCC
BcgI 2908 GCACGACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5575 CTTAAG C^TTAAG
BsaBI 3712 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 381 TCCGGA T^CCGGA
BsiEI 310 CGATCG CG[GA][CT]^CG
BspEI 381 TCCGGA T^CCGGA
BsrDI 549 GCAATG GCAATG(2/0)
Bst1107I 818 GTATAC GTA^TAC
BstZ17I 818 GTATAC GTA^TAC
DraI 5994 TTTAAA TTT^AAA
Eam1105I 5653 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HpaI 700 GTTAAC GTT^AAC
MamI 3712 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 381 TCCGGA T^CCGGA
NheI 1968 GCTAGC G^CTAGC
NspI 87 ACATGC [GA]CATG^[CT]
PflFI 979 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4165 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 310 CGATCG CGAT^CG
SapI 5398 GAAGAGC GCTCTTC(1/4)
SbfI 4328 CCTGCAGG CCTGCA^GG
SpeI 4141 ACTAGT A^CTAGT
Sse8387I 4328 CCTGCAGG CCTGCA^GG
Tth111I 979 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2293 GGTACC G^GTACC
4488 GGTACC
AccII 728 CGCG CG^CG
1983 CGCG
AcsI 2269 GAATTC [GA]^AATT[CT]
3082 GAATTC
AcyI 205 GACGCC G[GA]^CG[CT]C
4970 GACGCC
Alw44I 1530 GTGCAC G^TGCAC
3827 GTGCAC
AocI 3451 CCTCAGG CC^T[ACGT]AGG
4174 CCTCAGG
ApaI 4594 GGGCCC GGGCC^C
5372 GGGCCC
ApaLI 1530 GTGCAC G^TGCAC
3827 GTGCAC
ApoI 2269 GAATTC [GA]^AATT[CT]
3082 GAATTC
Asp718I 2293 GGTACC G^GTACC
4488 GGTACC
AvrII 2731 CCTAGG C^CTAGG
4019 CCTAGG
BclI 1166 TGATCA T^GATCA
3702 TGATCA
BlnI 2731 CCTAGG C^CTAGG
4019 CCTAGG
BsaAI 1154 TACGTG [CT]AC^GT[GA]
5604 TACGTG
BsaHI 205 GACGCC G[GA]^CG[CT]C
4970 GACGCC
BsaWI 381 TCCGGA [AT]^CCGG[AT]
2831 ACCGGA
BsmI 826 GAATGC GAATGC(1/-1)
4667 GCATTC
Bsp1407I 595 TGTACA T^GTACA
5899 TGTACA
BsrBI 437 GAGCGG CCGCTC(-3/-3)
3959 GAGCGG
BsrGI 595 TGTACA T^GTACA
5899 TGTACA
BssSI 2682 CTCGTG CACGAG(-5/-1)
3102 CTCGTG
BstUI 728 CGCG CG^CG
1983 CGCG
BstXI 3335 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5357 CCAGGGCCGTGG
Bsu36I 3451 CCTCAGG CC^T[ACGT]AGG
4174 CCTCAGG
Eco81I 3451 CCTCAGG CC^T[ACGT]AGG
4174 CCTCAGG
EcoRI 2269 GAATTC G^AATTC
3082 GAATTC
FbaI 1166 TGATCA T^GATCA
3702 TGATCA
Hin1I 205 GACGCC G[GA]^CG[CT]C
4970 GACGCC
HincII 700 GTTAAC GT[CT]^[GA]AC
1105 GTTGAC
HindII 700 GTTAAC GT[CT]^[GA]AC
1105 GTTGAC
HindIII 386 AAGCTT A^AGCTT
5877 AAGCTT
KpnI 2293 GGTACC GGTAC^C
4488 GGTACC
MvnI 728 CGCG CG^CG
1983 CGCG
NaeI 1684 GCCGGC GCC^GGC
5159 GCCGGC
NgoMIV 1684 GCCGGC G^CCGGC
5159 GCCGGC
SspBI 595 TGTACA T^GTACA
5899 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI