################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AclI AflIII AgeI Aor51HI AscI AseI AsnI AviII BbeI BbrPI BsiWI BspDI BspLU11I BssHII BstBI ClaI CpoI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI KasI KspI MfeI MluI MunI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccI 818 GTATAC GT^[AC][GT]AC AccIII 381 TCCGGA T^CCGGA AflII 5575 CTTAAG C^TTAAG AhdI 5653 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 5653 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 979 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 4130 GGATCC G^GATCC BcgI 2908 GCACGACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 5575 CTTAAG C^TTAAG BsaBI 3712 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 381 TCCGGA T^CCGGA BsiEI 310 CGATCG CG[GA][CT]^CG BspEI 381 TCCGGA T^CCGGA BsrDI 549 GCAATG GCAATG(2/0) Bst1107I 818 GTATAC GTA^TAC BstZ17I 818 GTATAC GTA^TAC DraI 5994 TTTAAA TTT^AAA Eam1105I 5653 GACCATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HpaI 700 GTTAAC GTT^AAC MamI 3712 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 381 TCCGGA T^CCGGA NheI 1968 GCTAGC G^CTAGC NspI 87 ACATGC [GA]CATG^[CT] PflFI 979 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4165 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 310 CGATCG CGAT^CG SapI 5398 GAAGAGC GCTCTTC(1/4) SbfI 4328 CCTGCAGG CCTGCA^GG SpeI 4141 ACTAGT A^CTAGT Sse8387I 4328 CCTGCAGG CCTGCA^GG Tth111I 979 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 2293 GGTACC G^GTACC 4488 GGTACC AccII 728 CGCG CG^CG 1983 CGCG AcsI 2269 GAATTC [GA]^AATT[CT] 3082 GAATTC AcyI 205 GACGCC G[GA]^CG[CT]C 4970 GACGCC Alw44I 1530 GTGCAC G^TGCAC 3827 GTGCAC AocI 3451 CCTCAGG CC^T[ACGT]AGG 4174 CCTCAGG ApaI 4594 GGGCCC GGGCC^C 5372 GGGCCC ApaLI 1530 GTGCAC G^TGCAC 3827 GTGCAC ApoI 2269 GAATTC [GA]^AATT[CT] 3082 GAATTC Asp718I 2293 GGTACC G^GTACC 4488 GGTACC AvrII 2731 CCTAGG C^CTAGG 4019 CCTAGG BclI 1166 TGATCA T^GATCA 3702 TGATCA BlnI 2731 CCTAGG C^CTAGG 4019 CCTAGG BsaAI 1154 TACGTG [CT]AC^GT[GA] 5604 TACGTG BsaHI 205 GACGCC G[GA]^CG[CT]C 4970 GACGCC BsaWI 381 TCCGGA [AT]^CCGG[AT] 2831 ACCGGA BsmI 826 GAATGC GAATGC(1/-1) 4667 GCATTC Bsp1407I 595 TGTACA T^GTACA 5899 TGTACA BsrBI 437 GAGCGG CCGCTC(-3/-3) 3959 GAGCGG BsrGI 595 TGTACA T^GTACA 5899 TGTACA BssSI 2682 CTCGTG CACGAG(-5/-1) 3102 CTCGTG BstUI 728 CGCG CG^CG 1983 CGCG BstXI 3335 CCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5357 CCAGGGCCGTGG Bsu36I 3451 CCTCAGG CC^T[ACGT]AGG 4174 CCTCAGG Eco81I 3451 CCTCAGG CC^T[ACGT]AGG 4174 CCTCAGG EcoRI 2269 GAATTC G^AATTC 3082 GAATTC FbaI 1166 TGATCA T^GATCA 3702 TGATCA Hin1I 205 GACGCC G[GA]^CG[CT]C 4970 GACGCC HincII 700 GTTAAC GT[CT]^[GA]AC 1105 GTTGAC HindII 700 GTTAAC GT[CT]^[GA]AC 1105 GTTGAC HindIII 386 AAGCTT A^AGCTT 5877 AAGCTT KpnI 2293 GGTACC GGTAC^C 4488 GGTACC MvnI 728 CGCG CG^CG 1983 CGCG NaeI 1684 GCCGGC GCC^GGC 5159 GCCGGC NgoMIV 1684 GCCGGC G^CCGGC 5159 GCCGGC SspBI 595 TGTACA T^GTACA 5899 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI