##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AflII Alw44I Aor51HI ApaI
ApaLI AscI AviII BcgI BfrI
BglII BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BspEI BssHII BstBI
BstEII BstPI CelII CpoI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI HpaI KspI MamI MfeI
MluI MroI MunI NotI NruI
NspV PacI PaeR7I PmeI PvuI
RsrII SacII SalI SapI SfiI
SfuI SgrAI SmaI SnaBI StuI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2391 GGTACC G^GTACC
AccII 2415 CGCG CG^CG
AclI 4618 AACGTT AA^CGTT
AgeI 302 ACCGGT A^CCGGT
AocI 2928 CCTCAGG CC^T[ACGT]AGG
Asp718I 2391 GGTACC G^GTACC
AspI 3465 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 975 CCCGAG C^[CT]CG[GA]G
BbeI 4535 GGCGCC GGCGC^C
BbrPI 342 CACGTG CAC^GTG
BclI 6071 TGATCA T^GATCA
BglI 945 GCCATACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 302 ACCGGT [AT]^CCGG[AT]
BsmBI 5522 CGTCTC CGTCTC(1/5)
BsoBI 975 CCCGAG C^[CT]CG[GA]G
BspDI 2519 ATCGAT AT^CGAT
BssSI 788 CTCGTG CACGAG(-5/-1)
Bst1107I 4272 GTATAC GTA^TAC
BstAPI 4074 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2415 CGCG CG^CG
BstZ17I 4272 GTATAC GTA^TAC
Bsu36I 2928 CCTCAGG CC^T[ACGT]AGG
ClaI 2519 ATCGAT AT^CGAT
DraIII 3283 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2928 CCTCAGG CC^T[ACGT]AGG
EcoNI 5843 CCTCAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 28 GATATC GAT^ATC
EcoT22I 4448 ATGCAT ATGCA^T
FbaI 6071 TGATCA T^GATCA
KasI 4535 GGCGCC G^GCGCC
KpnI 2391 GGTACC GGTAC^C
MvnI 2415 CGCG CG^CG
NarI 4535 GGCGCC GG^CGCC
NdeI 3671 CATATG CA^TATG
NheI 5968 GCTAGC G^CTAGC
NsiI 4448 ATGCAT ATGCA^T
PflFI 3465 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 302 ACCGGT A^CCGGT
PmaCI 342 CACGTG CAC^GTG
PmlI 342 CACGTG CAC^GTG
Psp1406I 4618 AACGTT AA^CGTT
ScaI 1299 AGTACT AGT^ACT
SexAI 5793 ACCAGGT A^CC[AT]GGT
SfoI 4535 GGCGCC GGC^GCC
SpeI 3971 ACTAGT A^CTAGT
SphI 2500 GCATGC GCATG^C
Tth111I 3465 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1684 GACGTC GACGT^C
3801 GACGTC
AhdI 1465 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5178 GACTCCATGTC
AlwNI 1360 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
1737 CAGCTCCTG
AseI 6220 ATTAAT AT^TAAT
6340 ATTAAT
AsnI 6220 ATTAAT AT^TAAT
6340 ATTAAT
AspEI 1465 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5178 GACTCCATGTC
BbvCI 398 CCTCAGC CCTCAGC(-5/-2)
1621 CCTCAGC
BciVI 3676 GGATAC GTATCC(6/5)
4116 GTATCC
BspLU11I 863 ACATGT A^CATGT
6334 ACATGT
BsrBI 1198 CCGCTC CCGCTC(-3/-3)
647 GAGCGG
DraI 4984 TTTAAA TTT^AAA
6436 TTTAAA
Eam1105I 1465 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5178 GACTCCATGTC
EcoRI 130 GAATTC G^AATTC
3767 GAATTC
FauI 5072 CCCGC CCCGC(4/6)
1510 GCGGG
HaeII 4535 GGCGCC [GA]GCGC^[CT]
5871 AGCGCC
HincII 5144 GTCAAC GT[CT]^[GA]AC
5298 GTCAAC
HindII 5144 GTCAAC GT[CT]^[GA]AC
5298 GTCAAC
HindIII 2161 AAGCTT A^AGCTT
3201 AAGCTT
PflMI 1430 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2528 CCAGCTTTTGG
PshBI 6220 ATTAAT AT^TAAT
6340 ATTAAT
SacI 385 GAGCTC GAGCT^C
3860 GAGCTC
SbfI 58 CCTGCAGG CCTGCA^GG
814 CCTGCAGG
Sse8387I 58 CCTGCAGG CCTGCA^GG
814 CCTGCAGG
Van91I 1430 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2528 CCAGCTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaII AvrII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BlnI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PshAI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI