##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AscI Asp718I AspEI AvrII BbeI
BcgI BfrI BlnI BseAI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 1028 ACACGT A^C[GA][CT]GT
AgeI 1898 ACCGGT A^CCGGT
Alw44I 1024 GTGCAC G^TGCAC
AocI 4062 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2092 AGCGCT AGC^GCT
ApaLI 1024 GTGCAC G^TGCAC
AseI 181 ATTAAT AT^TAAT
AsnI 181 ATTAAT AT^TAAT
AspI 3242 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 671 TGCGCA TGC^GCA
BbrPI 937 CACGTG CAC^GTG
BsaWI 1898 ACCGGT [AT]^CCGG[AT]
BsgI 249 CTGCAC GTGCAG(16/14)
BsiEI 2490 CGGCCG CG[GA][CT]^CG
BsiWI 1079 CGTACG C^GTACG
BsrDI 2449 GCAATG GCAATG(2/0)
BssHII 1887 GCGCGC G^CGCGC
BstAPI 3834 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2457 GGTAACC G^GT[ACGT]ACC
BstPI 2457 GGTAACC G^GT[ACGT]ACC
Bsu36I 4062 CCTCAGG CC^T[ACGT]AGG
DrdI 3608 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2490 CGGCCG C^GGCCG
EclXI 2490 CGGCCG C^GGCCG
Eco47III 2092 AGCGCT AGC^GCT
Eco52I 2490 CGGCCG C^GGCCG
Eco81I 4062 CCTCAGG CC^T[ACGT]AGG
EcoNI 3764 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2457 GGTAACC G^GT[ACGT]ACC
FspI 671 TGCGCA TGC^GCA
HincII 1538 GTCGAC GT[CT]^[GA]AC
HindII 1538 GTCGAC GT[CT]^[GA]AC
NaeI 1466 GCCGGC GCC^GGC
NgoMIV 1466 GCCGGC G^CCGGC
PflFI 3242 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1898 ACCGGT A^CCGGT
PmaCI 937 CACGTG CAC^GTG
PmlI 937 CACGTG CAC^GTG
PshBI 181 ATTAAT AT^TAAT
SalI 1538 GTCGAC G^TCGAC
SapI 463 GAAGAGC GCTCTTC(1/4)
SfiI 3102 GGCCCATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 162 GCATGC GCATG^C
SspI 1300 AATATT AAT^ATT
Tth111I 3242 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 661 GACGTC GACGT^C
2581 GACGTC
AccII 1182 CGCG CG^CG
1888 CGCG
Asp700I 120 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
594 GAATGAGTTC
BbvCI 12 CCTCAGC CCTCAGC(-5/-2)
789 CCTCAGC
BglII 392 AGATCT A^GATCT
2600 AGATCT
BlpI 1580 GCTCAGC GC^T[ACGT]AGC
2835 GCTCAGC
Bpu1102I 1580 GCTCAGC GC^T[ACGT]AGC
2835 GCTCAGC
BsmBI 1356 GAGACG CGTCTC(1/5)
1846 GAGACG
BsrFI 1466 GCCGGC [GA]^CCGG[CT]
1898 ACCGGT
BssSI 2660 CACGAG CACGAG(-5/-1)
3446 CTCGTG
BstUI 1182 CGCG CG^CG
1888 CGCG
CelII 1580 GCTCAGC GC^T[ACGT]AGC
2835 GCTCAGC
Cfr10I 1466 GCCGGC [GA]^CCGG[CT]
1898 ACCGGT
MvnI 1182 CGCG CG^CG
1888 CGCG
PpuMI 277 GGGACCT [GA]G^G[AT]CC[CT]
2646 GGGACCC
StuI 776 AGGCCT AGG^CCT
1805 AGGCCT
TfiI 687 GATTC G^A[AT]TC
2961 GAATC
XmnI 120 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
594 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BciVI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII