################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## Acc65I AccIII AclI AflII AhdI AscI Asp718I AspEI AvrII BbeI BcgI BfrI BlnI BseAI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 1028 ACACGT A^C[GA][CT]GT AgeI 1898 ACCGGT A^CCGGT Alw44I 1024 GTGCAC G^TGCAC AocI 4062 CCTCAGG CC^T[ACGT]AGG Aor51HI 2092 AGCGCT AGC^GCT ApaLI 1024 GTGCAC G^TGCAC AseI 181 ATTAAT AT^TAAT AsnI 181 ATTAAT AT^TAAT AspI 3242 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 671 TGCGCA TGC^GCA BbrPI 937 CACGTG CAC^GTG BsaWI 1898 ACCGGT [AT]^CCGG[AT] BsgI 249 CTGCAC GTGCAG(16/14) BsiEI 2490 CGGCCG CG[GA][CT]^CG BsiWI 1079 CGTACG C^GTACG BsrDI 2449 GCAATG GCAATG(2/0) BssHII 1887 GCGCGC G^CGCGC BstAPI 3834 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2457 GGTAACC G^GT[ACGT]ACC BstPI 2457 GGTAACC G^GT[ACGT]ACC Bsu36I 4062 CCTCAGG CC^T[ACGT]AGG DrdI 3608 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2490 CGGCCG C^GGCCG EclXI 2490 CGGCCG C^GGCCG Eco47III 2092 AGCGCT AGC^GCT Eco52I 2490 CGGCCG C^GGCCG Eco81I 4062 CCTCAGG CC^T[ACGT]AGG EcoNI 3764 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2457 GGTAACC G^GT[ACGT]ACC FspI 671 TGCGCA TGC^GCA HincII 1538 GTCGAC GT[CT]^[GA]AC HindII 1538 GTCGAC GT[CT]^[GA]AC NaeI 1466 GCCGGC GCC^GGC NgoMIV 1466 GCCGGC G^CCGGC PflFI 3242 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1898 ACCGGT A^CCGGT PmaCI 937 CACGTG CAC^GTG PmlI 937 CACGTG CAC^GTG PshBI 181 ATTAAT AT^TAAT SalI 1538 GTCGAC G^TCGAC SapI 463 GAAGAGC GCTCTTC(1/4) SfiI 3102 GGCCCATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 162 GCATGC GCATG^C SspI 1300 AATATT AAT^ATT Tth111I 3242 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AatII 661 GACGTC GACGT^C 2581 GACGTC AccII 1182 CGCG CG^CG 1888 CGCG Asp700I 120 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 594 GAATGAGTTC BbvCI 12 CCTCAGC CCTCAGC(-5/-2) 789 CCTCAGC BglII 392 AGATCT A^GATCT 2600 AGATCT BlpI 1580 GCTCAGC GC^T[ACGT]AGC 2835 GCTCAGC Bpu1102I 1580 GCTCAGC GC^T[ACGT]AGC 2835 GCTCAGC BsmBI 1356 GAGACG CGTCTC(1/5) 1846 GAGACG BsrFI 1466 GCCGGC [GA]^CCGG[CT] 1898 ACCGGT BssSI 2660 CACGAG CACGAG(-5/-1) 3446 CTCGTG BstUI 1182 CGCG CG^CG 1888 CGCG CelII 1580 GCTCAGC GC^T[ACGT]AGC 2835 GCTCAGC Cfr10I 1466 GCCGGC [GA]^CCGG[CT] 1898 ACCGGT MvnI 1182 CGCG CG^CG 1888 CGCG PpuMI 277 GGGACCT [GA]G^G[AT]CC[CT] 2646 GGGACCC StuI 776 AGGCCT AGG^CCT 1805 AGGCCT TfiI 687 GATTC G^A[AT]TC 2961 GAATC XmnI 120 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 594 GAATGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII