##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AhdI        
AscI        Asp718I     AspEI       AvrII       BbeI        
BcgI        BfrI        BlnI        BseAI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       Bst1107I    BstBI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
Eam1105I    EcoRI       EcoRV       EcoT22I     FseI        
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PmeI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SbfI        
ScaI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflIII       1028  ACACGT               A^C[GA][CT]GT
AgeI         1898  ACCGGT               A^CCGGT
Alw44I       1024  GTGCAC               G^TGCAC
AocI         4062  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      2092  AGCGCT               AGC^GCT
ApaLI        1024  GTGCAC               G^TGCAC
AseI          181  ATTAAT               AT^TAAT
AsnI          181  ATTAAT               AT^TAAT
AspI         3242  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         671  TGCGCA               TGC^GCA
BbrPI         937  CACGTG               CAC^GTG
BsaWI        1898  ACCGGT               [AT]^CCGG[AT]
BsgI          249  CTGCAC               GTGCAG(16/14)
BsiEI        2490  CGGCCG               CG[GA][CT]^CG
BsiWI        1079  CGTACG               C^GTACG
BsrDI        2449  GCAATG               GCAATG(2/0)
BssHII       1887  GCGCGC               G^CGCGC
BstAPI       3834  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2457  GGTAACC              G^GT[ACGT]ACC
BstPI        2457  GGTAACC              G^GT[ACGT]ACC
Bsu36I       4062  CCTCAGG              CC^T[ACGT]AGG
DrdI         3608  GACTGGGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2490  CGGCCG               C^GGCCG
EclXI        2490  CGGCCG               C^GGCCG
Eco47III     2092  AGCGCT               AGC^GCT
Eco52I       2490  CGGCCG               C^GGCCG
Eco81I       4062  CCTCAGG              CC^T[ACGT]AGG
EcoNI        3764  CCTTTAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2457  GGTAACC              G^GT[ACGT]ACC
FspI          671  TGCGCA               TGC^GCA
HincII       1538  GTCGAC               GT[CT]^[GA]AC
HindII       1538  GTCGAC               GT[CT]^[GA]AC
NaeI         1466  GCCGGC               GCC^GGC
NgoMIV       1466  GCCGGC               G^CCGGC
PflFI        3242  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1898  ACCGGT               A^CCGGT
PmaCI         937  CACGTG               CAC^GTG
PmlI          937  CACGTG               CAC^GTG
PshBI         181  ATTAAT               AT^TAAT
SalI         1538  GTCGAC               G^TCGAC
SapI          463  GAAGAGC              GCTCTTC(1/4)
SfiI         3102  GGCCCATGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          162  GCATGC               GCATG^C
SspI         1300  AATATT               AAT^ATT
Tth111I      3242  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AatII         661  GACGTC               GACGT^C
             2581  GACGTC              
AccII        1182  CGCG                 CG^CG
             1888  CGCG                
Asp700I       120  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              594  GAATGAGTTC          
BbvCI          12  CCTCAGC              CCTCAGC(-5/-2)
              789  CCTCAGC             
BglII         392  AGATCT               A^GATCT
             2600  AGATCT              
BlpI         1580  GCTCAGC              GC^T[ACGT]AGC
             2835  GCTCAGC             
Bpu1102I     1580  GCTCAGC              GC^T[ACGT]AGC
             2835  GCTCAGC             
BsmBI        1356  GAGACG               CGTCTC(1/5)
             1846  GAGACG              
BsrFI        1466  GCCGGC               [GA]^CCGG[CT]
             1898  ACCGGT              
BssSI        2660  CACGAG               CACGAG(-5/-1)
             3446  CTCGTG              
BstUI        1182  CGCG                 CG^CG
             1888  CGCG                
CelII        1580  GCTCAGC              GC^T[ACGT]AGC
             2835  GCTCAGC             
Cfr10I       1466  GCCGGC               [GA]^CCGG[CT]
             1898  ACCGGT              
MvnI         1182  CGCG                 CG^CG
             1888  CGCG                
PpuMI         277  GGGACCT              [GA]G^G[AT]CC[CT]
             2646  GGGACCC             
StuI          776  AGGCCT               AGG^CCT
             1805  AGGCCT              
TfiI          687  GATTC                G^A[AT]TC
             2961  GAATC               
XmnI          120  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              594  GAATGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvI        BciVI       
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MamI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PshAI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII