################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII BbeI BbrPI BcgI BglI BglII BsaBI BsiWI BsmBI BspDI BsrBI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI Eam1105I EcoO65I EcoRI FseI FspI HindIII HpaI KasI MamI MfeI MluI MunI NarI NcoI NotI NruI NspV PacI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SalI SbfI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspI StuI SwaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 2082 GACGTC GACGT^C Acc65I 2963 GGTACC G^GTACC AccI 2301 GTCTAC GT^[AC][GT]AC AccIII 2341 TCCGGA T^CCGGA AclI 4885 AACGTT AA^CGTT AcyI 2082 GACGTC G[GA]^CG[CT]C AflII 602 CTTAAG C^TTAAG Aor51HI 1489 AGCGCT AGC^GCT ApaI 176 GGGCCC GGGCC^C Asp700I 4702 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2963 GGTACC G^GTACC AspHI 4349 GAGCTC G[AT]GC[AT]^C AvrII 2979 CCTAGG C^CTAGG BalI 4371 TGGCCA TGG^CCA BbvCI 3331 GCTGAGG CCTCAGC(-5/-2) BclI 883 TGATCA T^GATCA BfrI 602 CTTAAG C^TTAAG BlnI 2979 CCTAGG C^CTAGG BsaHI 2082 GACGTC G[GA]^CG[CT]C BseAI 2341 TCCGGA T^CCGGA BsgI 3915 GTGCAG GTGCAG(16/14) BsiEI 95 CGGCCG CG[GA][CT]^CG BsiHKAI 4349 GAGCTC G[AT]GC[AT]^C Bsp1407I 2684 TGTACA T^GTACA BspEI 2341 TCCGGA T^CCGGA BspHI 3513 TCATGA T^CATGA BsrGI 2684 TGTACA T^GTACA BssHII 156 GCGCGC G^CGCGC BstAPI 822 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EagI 95 CGGCCG C^GGCCG EclXI 95 CGGCCG C^GGCCG Eco47III 1489 AGCGCT AGC^GCT Eco52I 95 CGGCCG C^GGCCG EcoRV 4447 GATATC GAT^ATC FbaI 883 TGATCA T^GATCA Hin1I 2082 GACGTC G[GA]^CG[CT]C HincII 948 GTCAAC GT[CT]^[GA]AC HindII 948 GTCAAC GT[CT]^[GA]AC KpnI 2963 GGTACC GGTAC^C KspI 34 CCGCGG CCGC^GG MluNI 4371 TGGCCA TGG^CCA MroI 2341 TCCGGA T^CCGGA MscI 4371 TGGCCA TGG^CCA NaeI 97 GCCGGC GCC^GGC NdeI 970 CATATG CA^TATG NgoMIV 97 GCCGGC G^CCGGC PpuMI 1705 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 4885 AACGTT AA^CGTT PvuII 208 CAGCTG CAG^CTG RcaI 3513 TCATGA T^CATGA SacI 4349 GAGCTC GAGCT^C SacII 34 CCGCGG CCGC^GG SexAI 297 ACCTGGT A^CC[AT]GGT SmaI 4420 CCCGGG CCC^GGG SnaBI 1606 TACGTA TAC^GTA SphI 1927 GCATGC GCATG^C SspBI 2684 TGTACA T^GTACA XbaI 3713 TCTAGA T^CTAGA XcmI 3122 CCACTTGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 4420 CCCGGG C^CCGGG XmaI 4420 CCCGGG C^CCGGG XmnI 4702 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 1810 ACATGT A^C[GA][CT]GT 3457 ACATGT AgeI 247 ACCGGT A^CCGGT 640 ACCGGT AocI 777 CCTGAGG CC^T[ACGT]AGG 4752 CCTGAGG AspI 296 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3391 GACACTGTC BamHI 586 GGATCC G^GATCC 2224 GGATCC BsaAI 876 CACGTA [CT]AC^GT[GA] 1606 TACGTA BsaI 1311 GAGACC GGTCTC(1/5) 2948 GGTCTC BspLU11I 1810 ACATGT A^CATGT 3457 ACATGT BsrDI 2765 CATTGC GCAATG(2/0) 1885 GCAATG BstXI 1648 CCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2425 CCACGCCTTTGG Bsu36I 777 CCTGAGG CC^T[ACGT]AGG 4752 CCTGAGG DraI 3157 TTTAAA TTT^AAA 4615 TTTAAA DraII 27 AGGCCCC [GA]G^G[ACGT]CC[CT] 1705 AGGACCT EaeI 95 CGGCCG [CT]^GGCC[GA] 4371 TGGCCA Eco81I 777 CCTGAGG CC^T[ACGT]AGG 4752 CCTGAGG EcoNI 2974 CCTTGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3083 CCTCCAGCAGG EcoO109I 27 AGGCCCC [GA]G^G[ACGT]CC[CT] 1705 AGGACCT EcoT22I 1548 ATGCAT ATGCA^T 4926 ATGCAT HgaI 2243 GACGC GACGC(5/10) 59 GCGTC NheI 4011 GCTAGC G^CTAGC 4365 GCTAGC NsiI 1548 ATGCAT ATGCA^T 4926 ATGCAT PaeR7I 2122 CTCGAG C^TCGAG 2452 CTCGAG PflFI 296 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3391 GACACTGTC PflMI 75 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4309 CCAGAAAGTGG PinAI 247 ACCGGT A^CCGGT 640 ACCGGT ScaI 1684 AGTACT AGT^ACT 4053 AGTACT Tth111I 296 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3391 GACACTGTC Van91I 75 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4309 CCAGAAAGTGG XhoI 2122 CTCGAG C^TCGAG 2452 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII