##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AviII BbeI BbrPI
BcgI BglI BglII BsaBI BsiWI
BsmBI BspDI BsrBI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I EcoO65I
EcoRI FseI FspI HindIII HpaI
KasI MamI MfeI MluI MunI
NarI NcoI NotI NruI NspV
PacI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SpeI
Sse8387I SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 2082 GACGTC GACGT^C
Acc65I 2963 GGTACC G^GTACC
AccI 2301 GTCTAC GT^[AC][GT]AC
AccIII 2341 TCCGGA T^CCGGA
AclI 4885 AACGTT AA^CGTT
AcyI 2082 GACGTC G[GA]^CG[CT]C
AflII 602 CTTAAG C^TTAAG
Aor51HI 1489 AGCGCT AGC^GCT
ApaI 176 GGGCCC GGGCC^C
Asp700I 4702 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2963 GGTACC G^GTACC
AspHI 4349 GAGCTC G[AT]GC[AT]^C
AvrII 2979 CCTAGG C^CTAGG
BalI 4371 TGGCCA TGG^CCA
BbvCI 3331 GCTGAGG CCTCAGC(-5/-2)
BclI 883 TGATCA T^GATCA
BfrI 602 CTTAAG C^TTAAG
BlnI 2979 CCTAGG C^CTAGG
BsaHI 2082 GACGTC G[GA]^CG[CT]C
BseAI 2341 TCCGGA T^CCGGA
BsgI 3915 GTGCAG GTGCAG(16/14)
BsiEI 95 CGGCCG CG[GA][CT]^CG
BsiHKAI 4349 GAGCTC G[AT]GC[AT]^C
Bsp1407I 2684 TGTACA T^GTACA
BspEI 2341 TCCGGA T^CCGGA
BspHI 3513 TCATGA T^CATGA
BsrGI 2684 TGTACA T^GTACA
BssHII 156 GCGCGC G^CGCGC
BstAPI 822 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 95 CGGCCG C^GGCCG
EclXI 95 CGGCCG C^GGCCG
Eco47III 1489 AGCGCT AGC^GCT
Eco52I 95 CGGCCG C^GGCCG
EcoRV 4447 GATATC GAT^ATC
FbaI 883 TGATCA T^GATCA
Hin1I 2082 GACGTC G[GA]^CG[CT]C
HincII 948 GTCAAC GT[CT]^[GA]AC
HindII 948 GTCAAC GT[CT]^[GA]AC
KpnI 2963 GGTACC GGTAC^C
KspI 34 CCGCGG CCGC^GG
MluNI 4371 TGGCCA TGG^CCA
MroI 2341 TCCGGA T^CCGGA
MscI 4371 TGGCCA TGG^CCA
NaeI 97 GCCGGC GCC^GGC
NdeI 970 CATATG CA^TATG
NgoMIV 97 GCCGGC G^CCGGC
PpuMI 1705 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 4885 AACGTT AA^CGTT
PvuII 208 CAGCTG CAG^CTG
RcaI 3513 TCATGA T^CATGA
SacI 4349 GAGCTC GAGCT^C
SacII 34 CCGCGG CCGC^GG
SexAI 297 ACCTGGT A^CC[AT]GGT
SmaI 4420 CCCGGG CCC^GGG
SnaBI 1606 TACGTA TAC^GTA
SphI 1927 GCATGC GCATG^C
SspBI 2684 TGTACA T^GTACA
XbaI 3713 TCTAGA T^CTAGA
XcmI 3122 CCACTTGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4420 CCCGGG C^CCGGG
XmaI 4420 CCCGGG C^CCGGG
XmnI 4702 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1810 ACATGT A^C[GA][CT]GT
3457 ACATGT
AgeI 247 ACCGGT A^CCGGT
640 ACCGGT
AocI 777 CCTGAGG CC^T[ACGT]AGG
4752 CCTGAGG
AspI 296 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3391 GACACTGTC
BamHI 586 GGATCC G^GATCC
2224 GGATCC
BsaAI 876 CACGTA [CT]AC^GT[GA]
1606 TACGTA
BsaI 1311 GAGACC GGTCTC(1/5)
2948 GGTCTC
BspLU11I 1810 ACATGT A^CATGT
3457 ACATGT
BsrDI 2765 CATTGC GCAATG(2/0)
1885 GCAATG
BstXI 1648 CCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2425 CCACGCCTTTGG
Bsu36I 777 CCTGAGG CC^T[ACGT]AGG
4752 CCTGAGG
DraI 3157 TTTAAA TTT^AAA
4615 TTTAAA
DraII 27 AGGCCCC [GA]G^G[ACGT]CC[CT]
1705 AGGACCT
EaeI 95 CGGCCG [CT]^GGCC[GA]
4371 TGGCCA
Eco81I 777 CCTGAGG CC^T[ACGT]AGG
4752 CCTGAGG
EcoNI 2974 CCTTGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3083 CCTCCAGCAGG
EcoO109I 27 AGGCCCC [GA]G^G[ACGT]CC[CT]
1705 AGGACCT
EcoT22I 1548 ATGCAT ATGCA^T
4926 ATGCAT
HgaI 2243 GACGC GACGC(5/10)
59 GCGTC
NheI 4011 GCTAGC G^CTAGC
4365 GCTAGC
NsiI 1548 ATGCAT ATGCA^T
4926 ATGCAT
PaeR7I 2122 CTCGAG C^TCGAG
2452 CTCGAG
PflFI 296 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3391 GACACTGTC
PflMI 75 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4309 CCAGAAAGTGG
PinAI 247 ACCGGT A^CCGGT
640 ACCGGT
ScaI 1684 AGTACT AGT^ACT
4053 AGTACT
Tth111I 296 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3391 GACACTGTC
Van91I 75 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4309 CCAGAAAGTGG
XhoI 2122 CTCGAG C^TCGAG
2452 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII