################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## Acc65I AclI AflIII AgeI AhdI ApaI AscI Asp718I AspEI BamHI BbeI BsiEI BsiWI BsmBI Bsp1407I BspLU11I BsrGI BssHII Bst1107I BstBI BstXI BstZ17I CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FseI HpaI KasI KpnI MfeI MluI MunI NarI NdeI NotI NruI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 3828 GACGTC GACGT^C AccI 2085 GTCTAC GT^[AC][GT]AC AccIII 3750 TCCGGA T^CCGGA AflII 4017 CTTAAG C^TTAAG Alw44I 5217 GTGCAC G^TGCAC AlwNI 2433 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 5217 GTGCAC G^TGCAC Asp700I 1005 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 4754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5572 TGCGCA TGC^GCA AvrII 1177 CCTAGG C^CTAGG BfrI 4017 CTTAAG C^TTAAG BglI 4470 GCCATTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1177 CCTAGG C^CTAGG BsaBI 2322 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3750 TCCGGA T^CCGGA BspDI 4614 ATCGAT AT^CGAT BspEI 3750 TCCGGA T^CCGGA BspHI 3655 TCATGA T^CATGA BsrBI 330 GAGCGG CCGCTC(-3/-3) ClaI 4614 ATCGAT AT^CGAT EcoNI 2114 CCTCGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1978 ATGCAT ATGCA^T FspI 5572 TGCGCA TGC^GCA HincII 5424 GTCAAC GT[CT]^[GA]AC HindII 5424 GTCAAC GT[CT]^[GA]AC HindIII 5398 AAGCTT A^AGCTT KspI 1554 CCGCGG CCGC^GG MamI 2322 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3750 TCCGGA T^CCGGA NaeI 5174 GCCGGC GCC^GGC NgoMIV 5174 GCCGGC G^CCGGC NheI 2723 GCTAGC G^CTAGC NsiI 1978 ATGCAT ATGCA^T PflFI 4754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 122 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4214 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1748 CAGCTG CAG^CTG RcaI 3655 TCATGA T^CATGA SacI 4385 GAGCTC GAGCT^C SacII 1554 CCGCGG CCGC^GG SphI 1269 GCATGC GCATG^C Tth111I 4754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 122 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2280 TCTAGA T^CTAGA XmnI 1005 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 1555 CGCG CG^CG 4120 CGCG AcsI 794 GAATTT [GA]^AATT[CT] 3651 AAATTC ApoI 794 GAATTT [GA]^AATT[CT] 3651 AAATTC AseI 42 ATTAAT AT^TAAT 458 ATTAAT AsnI 42 ATTAAT AT^TAAT 458 ATTAAT BbrPI 1895 CACGTG CAC^GTG 3792 CACGTG BbvCI 2136 CCTCAGC CCTCAGC(-5/-2) 5292 CCTCAGC BcgI 495 GCAGGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5325 GCAGCGGCCTCG BciVI 2898 GGATAC GTATCC(6/5) 4665 GTATCC BclI 3005 TGATCA T^GATCA 3646 TGATCA BcnI 4025 CCCGG CC^[GC]GG 5435 CCCGG BglII 97 AGATCT A^GATCT 2327 AGATCT BsaAI 1895 CACGTG [CT]AC^GT[GA] 3792 CACGTG BsaWI 727 ACCGGA [AT]^CCGG[AT] 3750 TCCGGA BstAPI 166 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1264 GCAGAGCATGC BstEII 4690 GGTCACC G^GT[ACGT]ACC 4979 GGTCACC BstPI 4690 GGTCACC G^GT[ACGT]ACC 4979 GGTCACC BstUI 1555 CGCG CG^CG 4120 CGCG DraI 26 TTTAAA TTT^AAA 604 TTTAAA EcoO65I 4690 GGTCACC G^GT[ACGT]ACC 4979 GGTCACC FbaI 3005 TGATCA T^GATCA 3646 TGATCA MvnI 1555 CGCG CG^CG 4120 CGCG NciI 4025 CCCGG CC^[GC]GG 5435 CCCGG NcoI 3661 CCATGG C^CATGG 4933 CCATGG NspI 1269 GCATGC [GA]CATG^[CT] 2313 ACATGC PmaCI 1895 CACGTG CAC^GTG 3792 CACGTG PmlI 1895 CACGTG CAC^GTG 3792 CACGTG PshBI 42 ATTAAT AT^TAAT 458 ATTAAT SspI 229 AATATT AAT^ATT 2006 AATATT StuI 2825 AGGCCT AGG^CCT 4630 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AocI Aor51HI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco47III Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII