##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
ApaI AscI Asp718I AspEI BamHI
BbeI BsiEI BsiWI BsmBI Bsp1407I
BspLU11I BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I CpoI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FseI
HpaI KasI KpnI MfeI MluI
MunI NarI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3828 GACGTC GACGT^C
AccI 2085 GTCTAC GT^[AC][GT]AC
AccIII 3750 TCCGGA T^CCGGA
AflII 4017 CTTAAG C^TTAAG
Alw44I 5217 GTGCAC G^TGCAC
AlwNI 2433 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 5217 GTGCAC G^TGCAC
Asp700I 1005 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5572 TGCGCA TGC^GCA
AvrII 1177 CCTAGG C^CTAGG
BfrI 4017 CTTAAG C^TTAAG
BglI 4470 GCCATTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1177 CCTAGG C^CTAGG
BsaBI 2322 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3750 TCCGGA T^CCGGA
BspDI 4614 ATCGAT AT^CGAT
BspEI 3750 TCCGGA T^CCGGA
BspHI 3655 TCATGA T^CATGA
BsrBI 330 GAGCGG CCGCTC(-3/-3)
ClaI 4614 ATCGAT AT^CGAT
EcoNI 2114 CCTCGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1978 ATGCAT ATGCA^T
FspI 5572 TGCGCA TGC^GCA
HincII 5424 GTCAAC GT[CT]^[GA]AC
HindII 5424 GTCAAC GT[CT]^[GA]AC
HindIII 5398 AAGCTT A^AGCTT
KspI 1554 CCGCGG CCGC^GG
MamI 2322 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3750 TCCGGA T^CCGGA
NaeI 5174 GCCGGC GCC^GGC
NgoMIV 5174 GCCGGC G^CCGGC
NheI 2723 GCTAGC G^CTAGC
NsiI 1978 ATGCAT ATGCA^T
PflFI 4754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 122 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4214 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1748 CAGCTG CAG^CTG
RcaI 3655 TCATGA T^CATGA
SacI 4385 GAGCTC GAGCT^C
SacII 1554 CCGCGG CCGC^GG
SphI 1269 GCATGC GCATG^C
Tth111I 4754 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 122 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2280 TCTAGA T^CTAGA
XmnI 1005 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1555 CGCG CG^CG
4120 CGCG
AcsI 794 GAATTT [GA]^AATT[CT]
3651 AAATTC
ApoI 794 GAATTT [GA]^AATT[CT]
3651 AAATTC
AseI 42 ATTAAT AT^TAAT
458 ATTAAT
AsnI 42 ATTAAT AT^TAAT
458 ATTAAT
BbrPI 1895 CACGTG CAC^GTG
3792 CACGTG
BbvCI 2136 CCTCAGC CCTCAGC(-5/-2)
5292 CCTCAGC
BcgI 495 GCAGGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5325 GCAGCGGCCTCG
BciVI 2898 GGATAC GTATCC(6/5)
4665 GTATCC
BclI 3005 TGATCA T^GATCA
3646 TGATCA
BcnI 4025 CCCGG CC^[GC]GG
5435 CCCGG
BglII 97 AGATCT A^GATCT
2327 AGATCT
BsaAI 1895 CACGTG [CT]AC^GT[GA]
3792 CACGTG
BsaWI 727 ACCGGA [AT]^CCGG[AT]
3750 TCCGGA
BstAPI 166 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1264 GCAGAGCATGC
BstEII 4690 GGTCACC G^GT[ACGT]ACC
4979 GGTCACC
BstPI 4690 GGTCACC G^GT[ACGT]ACC
4979 GGTCACC
BstUI 1555 CGCG CG^CG
4120 CGCG
DraI 26 TTTAAA TTT^AAA
604 TTTAAA
EcoO65I 4690 GGTCACC G^GT[ACGT]ACC
4979 GGTCACC
FbaI 3005 TGATCA T^GATCA
3646 TGATCA
MvnI 1555 CGCG CG^CG
4120 CGCG
NciI 4025 CCCGG CC^[GC]GG
5435 CCCGG
NcoI 3661 CCATGG C^CATGG
4933 CCATGG
NspI 1269 GCATGC [GA]CATG^[CT]
2313 ACATGC
PmaCI 1895 CACGTG CAC^GTG
3792 CACGTG
PmlI 1895 CACGTG CAC^GTG
3792 CACGTG
PshBI 42 ATTAAT AT^TAAT
458 ATTAAT
SspI 229 AATATT AAT^ATT
2006 AATATT
StuI 2825 AGGCCT AGG^CCT
4630 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AocI Aor51HI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco47III Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII