##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccI AccIII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AviII AvrII BalI BlnI BsaWI
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstZ17I Bsu36I ClaI CpoI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I FauI FseI FspI
HincII HindII HpaI KpnI MluI
MluNI MroI MscI NheI NotI
NruI PacI PinAI PmeI PvuI
RsrII SalI SbfI SfiI SgrAI
SmaI SnaBI Sse8387I SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 975 GACGTC GACGT^C
AccII 5151 CGCG CG^CG
AflII 2394 CTTAAG C^TTAAG
Alw44I 4781 GTGCAC G^TGCAC
ApaLI 4781 GTGCAC G^TGCAC
BamHI 253 GGATCC G^GATCC
BbeI 4622 GGCGCC GGCGC^C
BbrPI 1593 CACGTG CAC^GTG
BbvCI 4720 CCTCAGC CCTCAGC(-5/-2)
BciVI 3288 GTATCC GTATCC(6/5)
BfrI 2394 CTTAAG C^TTAAG
BglII 5268 AGATCT A^GATCT
BsaAI 1593 CACGTG [CT]AC^GT[GA]
BsiEI 893 CGGTCG CG[GA][CT]^CG
Bsp1407I 3542 TGTACA T^GTACA
BsrBI 2853 GAGCGG CCGCTC(-3/-3)
BsrDI 2999 CATTGC GCAATG(2/0)
BsrFI 100 GCCGGC [GA]^CCGG[CT]
BsrGI 3542 TGTACA T^GTACA
BssSI 2187 CTCGTG CACGAG(-5/-1)
BstUI 5151 CGCG CG^CG
Cfr10I 100 GCCGGC [GA]^CCGG[CT]
DraIII 4946 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 4772 GAATTC G^AATTC
EcoT22I 1549 ATGCAT ATGCA^T
KasI 4622 GGCGCC G^GCGCC
KspI 5150 CCGCGG CCGC^GG
MfeI 4208 CAATTG C^AATTG
MunI 4208 CAATTG C^AATTG
MvnI 5151 CGCG CG^CG
NaeI 100 GCCGGC GCC^GGC
NarI 4622 GGCGCC GG^CGCC
NdeI 2325 CATATG CA^TATG
NgoMIV 100 GCCGGC G^CCGGC
NsiI 1549 ATGCAT ATGCA^T
PflMI 3299 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1593 CACGTG CAC^GTG
PmlI 1593 CACGTG CAC^GTG
PstI 1521 CTGCAG CTGCA^G
SacII 5150 CCGCGG CCGC^GG
SapI 908 GCTCTTC GCTCTTC(1/4)
ScaI 3879 AGTACT AGT^ACT
SfoI 4622 GGCGCC GGC^GCC
SpeI 3809 ACTAGT A^CTAGT
SphI 649 GCATGC GCATG^C
SspBI 3542 TGTACA T^GTACA
Van91I 3299 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3718 TCTAGA T^CTAGA
XcmI 5093 CCATCTGGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AclI 3158 AACGTT AA^CGTT
3781 AACGTT
AcyI 975 GACGTC G[GA]^CG[CT]C
4622 GGCGCC
AflIII 132 ACATGT A^C[GA][CT]GT
2121 ACATGT
ApaI 2582 GGGCCC GGGCC^C
3051 GGGCCC
AseI 1064 ATTAAT AT^TAAT
3609 ATTAAT
AsnI 1064 ATTAAT AT^TAAT
3609 ATTAAT
AspI 236 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5085 GACCCTGTC
BanI 1097 GGTGCC G^G[CT][GA]CC
4622 GGCGCC
BbsI 3117 GAAGAC GAAGAC(2/6)
3199 GTCTTC
BcnI 820 CCGGG CC^[GC]GG
4667 CCCGG
BpuAI 3117 GAAGAC GAAGAC(2/6)
3199 GTCTTC
BsaBI 1539 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5233 GATTTGTATC
BsaHI 975 GACGTC G[GA]^CG[CT]C
4622 GGCGCC
BsmBI 1293 GAGACG CGTCTC(1/5)
2874 GAGACG
BspLU11I 132 ACATGT A^CATGT
2121 ACATGT
BstAPI 104 GCAAATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4233 GCATCGAGTGC
BstBI 772 TTCGAA TT^CGAA
1834 TTCGAA
BstEII 1478 GGTGACC G^GT[ACGT]ACC
5178 GGTGACC
BstPI 1478 GGTGACC G^GT[ACGT]ACC
5178 GGTGACC
BstXI 2199 CCAGACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5036 CCATGGAAATGG
DrdI 534 GACATATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1743 GACCAGAGGGTC
EcoO65I 1478 GGTGACC G^GT[ACGT]ACC
5178 GGTGACC
HgaI 1295 GACGC GACGC(5/10)
5137 GCGTC
Hin1I 975 GACGTC G[GA]^CG[CT]C
4622 GGCGCC
MamI 1539 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5233 GATTTGTATC
NciI 820 CCGGG CC^[GC]GG
4667 CCCGG
NspV 772 TTCGAA TT^CGAA
1834 TTCGAA
PaeR7I 859 CTCGAG C^TCGAG
1484 CTCGAG
PflFI 236 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5085 GACCCTGTC
PshAI 2031 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2876 GACGGTGGTC
PshBI 1064 ATTAAT AT^TAAT
3609 ATTAAT
Psp1406I 3158 AACGTT AA^CGTT
3781 AACGTT
SfuI 772 TTCGAA TT^CGAA
1834 TTCGAA
StuI 1817 AGGCCT AGG^CCT
2792 AGGCCT
Tth111I 236 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5085 GACCCTGTC
XhoI 859 CTCGAG C^TCGAG
1484 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanII BbvI BcgI BclI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoRV EcoT14I
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI