##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AocI AscI AseI AsnI Asp718I
AvrII BamHI BlnI BsaBI Bsp1407I
BspHI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CpoI
DraI DrdI Eco81I EcoO65I EcoRV
EcoT22I FseI KpnI KspI MamI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 1083 TCCGGA T^CCGGA
AflII 1830 CTTAAG C^TTAAG
AhdI 1236 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1371 GTGCAC G^TGCAC
Aor51HI 81 AGCGCT AGC^GCT
ApaI 3664 GGGCCC GGGCC^C
ApaLI 1371 GTGCAC G^TGCAC
AspEI 1236 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 855 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1556 GGCGCC GGCGC^C
BbrPI 4192 CACGTG CAC^GTG
BcgI 2932 CGACTCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 883 TGATCA T^GATCA
BfrI 1830 CTTAAG C^TTAAG
BlpI 3703 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3703 GCTCAGC GC^T[ACGT]AGC
BsaAI 4192 CACGTG [CT]AC^GT[GA]
BsaWI 1083 TCCGGA [AT]^CCGG[AT]
BseAI 1083 TCCGGA T^CCGGA
BsiEI 925 CGGCCG CG[GA][CT]^CG
BsiWI 709 CGTACG C^GTACG
BspEI 1083 TCCGGA T^CCGGA
BspLU11I 3522 ACATGT A^CATGT
BsrFI 21 GCCGGT [GA]^CCGG[CT]
BssHII 2986 GCGCGC G^CGCGC
BssSI 2018 CACGAG CACGAG(-5/-1)
CelII 3703 GCTCAGC GC^T[ACGT]AGC
Cfr10I 21 GCCGGT [GA]^CCGG[CT]
EagI 925 CGGCCG C^GGCCG
Eam1105I 1236 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 925 CGGCCG C^GGCCG
Eco47III 81 AGCGCT AGC^GCT
Eco52I 925 CGGCCG C^GGCCG
FbaI 883 TGATCA T^GATCA
HindIII 285 AAGCTT A^AGCTT
HpaI 2397 GTTAAC GTT^AAC
KasI 1556 GGCGCC G^GCGCC
MfeI 1926 CAATTG C^AATTG
MroI 1083 TCCGGA T^CCGGA
MunI 1926 CAATTG C^AATTG
NarI 1556 GGCGCC GG^CGCC
PflFI 855 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4192 CACGTG CAC^GTG
PmlI 4192 CACGTG CAC^GTG
PshAI 1496 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 1556 GGCGCC GGC^GCC
Tth111I 855 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2693 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 741 GGCGTC G[GA]^CG[CT]C
1556 GGCGCC
Asp700I 1013 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2743 GAAATCTTTC
AviII 223 TGCGCA TGC^GCA
1177 TGCGCA
BglII 496 AGATCT A^GATCT
1548 AGATCT
BsaHI 741 GGCGTC G[GA]^CG[CT]C
1556 GGCGCC
BsgI 158 CTGCAC GTGCAG(16/14)
3150 CTGCAC
BsmBI 4461 CGTCTC CGTCTC(1/5)
4232 GAGACG
BsmI 3117 GAATGC GAATGC(1/-1)
4539 GAATGC
BspDI 1327 ATCGAT AT^CGAT
1979 ATCGAT
ClaI 1327 ATCGAT AT^CGAT
1979 ATCGAT
DraIII 2650 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4177 CACTATGTG
EcoNI 1304 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3760 CCTTTACCAGG
EcoRI 1673 GAATTC G^AATTC
2260 GAATTC
FspI 223 TGCGCA TGC^GCA
1177 TGCGCA
Hin1I 741 GGCGTC G[GA]^CG[CT]C
1556 GGCGCC
HincII 2224 GTTGAC GT[CT]^[GA]AC
2397 GTTAAC
HindII 2224 GTTGAC GT[CT]^[GA]AC
2397 GTTAAC
NcoI 948 CCATGG C^CATGG
4498 CCATGG
PflMI 305 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1400 CCACCTGATGG
PstI 4019 CTGCAG CTGCA^G
4245 CTGCAG
SexAI 1303 ACCTGGT A^CC[AT]GGT
3934 ACCAGGT
SphI 982 GCATGC GCATG^C
2347 GCATGC
Van91I 305 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1400 CCACCTGATGG
XcmI 133 CCAGTGCCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
840 CCATCACCAGAATGG
XmnI 1013 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2743 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI