##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Aor51HI ApaI Asp718I AspEI
AvaI AviII AvrII BbeI BcgI
BfrI BlnI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BssSI BstBI
BstEII BstPI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI KasI
KpnI KspI MluI MroI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PmeI PpuMI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 130 GACGCC G[GA]^CG[CT]C
AgeI 3389 ACCGGT A^CCGGT
AscI 115 GGCGCGCC GG^CGCGCC
AspI 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 493 GGATCC G^GATCC
BanI 5249 GGTGCC G^G[CT][GA]CC
BanII 27 GAGCTC G[GA]GC[CT]^C
BbrPI 4963 CACGTG CAC^GTG
BbvCI 2764 CCTCAGC CCTCAGC(-5/-2)
BglI 61 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3055 AGATCT A^GATCT
BlpI 540 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 540 GCTTAGC GC^T[ACGT]AGC
BsaAI 4963 CACGTG [CT]AC^GT[GA]
BsaBI 2440 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 130 GACGCC G[GA]^CG[CT]C
BsaWI 3389 ACCGGT [AT]^CCGG[AT]
BspLU11I 3564 ACATGT A^CATGT
BsrFI 3389 ACCGGT [GA]^CCGG[CT]
BssHII 116 GCGCGC G^CGCGC
Bst1107I 2682 GTATAC GTA^TAC
BstXI 4412 CCAAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2682 GTATAC GTA^TAC
CelII 540 GCTTAGC GC^T[ACGT]AGC
Cfr10I 3389 ACCGGT [GA]^CCGG[CT]
DraII 15 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 6004 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1024 CCTAATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 15 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1828 ATGCAT ATGCA^T
HgaI 130 GACGC GACGC(5/10)
Hin1I 130 GACGCC G[GA]^CG[CT]C
HincII 5887 GTTAAC GT[CT]^[GA]AC
HindII 5887 GTTAAC GT[CT]^[GA]AC
HpaI 5887 GTTAAC GTT^AAC
MamI 2440 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 910 GCTAGC G^CTAGC
NsiI 1828 ATGCAT ATGCA^T
PflFI 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3389 ACCGGT A^CCGGT
PmaCI 4963 CACGTG CAC^GTG
PmlI 4963 CACGTG CAC^GTG
SacI 27 GAGCTC GAGCT^C
SapI 90 GCTCTTC GCTCTTC(1/4)
SexAI 1655 ACCTGGT A^CC[AT]GGT
SfiI 60 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 4528 ACTAGT A^CTAGT
SwaI 5012 ATTTAAAT ATTT^AAAT
Tth111I 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2682 GTATAC GT^[AC][GT]AC
3706 GTAGAC
AflIII 3564 ACATGT A^C[GA][CT]GT
4964 ACGTGT
Alw44I 4407 GTGCAC G^TGCAC
5563 GTGCAC
AocI 3435 CCTGAGG CC^T[ACGT]AGG
4309 CCTAAGG
ApaLI 4407 GTGCAC G^TGCAC
5563 GTGCAC
BalI 3245 TGGCCA TGG^CCA
4456 TGGCCA
BciVI 4314 GGATAC GTATCC(6/5)
2667 GTATCC
BsmBI 128 GAGACG CGTCTC(1/5)
3258 GAGACG
BsmI 2002 GAATGC GAATGC(1/-1)
4256 GCATTC
BspMI 3317 ACCTGC ACCTGC(4/8)
5455 GCAGGT
BstAPI 835 GCACGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6003 GCACGCAGTGC
Bsu36I 3435 CCTGAGG CC^T[ACGT]AGG
4309 CCTAAGG
DsaI 2805 CCATGG C^C[GA][CT]GG
5236 CCATGG
Eco81I 3435 CCTGAGG CC^T[ACGT]AGG
4309 CCTAAGG
HaeII 1597 GGCGCT [GA]GCGC^[CT]
3615 GGCGCT
HindIII 993 AAGCTT A^AGCTT
4603 AAGCTT
MluNI 3245 TGGCCA TGG^CCA
4456 TGGCCA
MscI 3245 TGGCCA TGG^CCA
4456 TGGCCA
NcoI 2805 CCATGG C^CATGG
5236 CCATGG
NdeI 4120 CATATG CA^TATG
4490 CATATG
PflMI 1297 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1912 CCAGTCCTTGG
PvuII 331 CAGCTG CAG^CTG
1205 CAGCTG
Van91I 1297 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1912 CCAGTCCTTGG
XbaI 3569 TCTAGA T^CTAGA
4300 TCTAGA
XcmI 5216 CCAGTTAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5236 CCATGGATGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshAI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI