################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AhdI Aor51HI ApaI Asp718I AspEI AvaI AviII AvrII BbeI BcgI BfrI BlnI BseAI BsiEI BsiWI BsoBI BspDI BspEI BssSI BstBI BstEII BstPI ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PmeI PpuMI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 130 GACGCC G[GA]^CG[CT]C AgeI 3389 ACCGGT A^CCGGT AscI 115 GGCGCGCC GG^CGCGCC AspI 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 493 GGATCC G^GATCC BanI 5249 GGTGCC G^G[CT][GA]CC BanII 27 GAGCTC G[GA]GC[CT]^C BbrPI 4963 CACGTG CAC^GTG BbvCI 2764 CCTCAGC CCTCAGC(-5/-2) BglI 61 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3055 AGATCT A^GATCT BlpI 540 GCTTAGC GC^T[ACGT]AGC Bpu1102I 540 GCTTAGC GC^T[ACGT]AGC BsaAI 4963 CACGTG [CT]AC^GT[GA] BsaBI 2440 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 130 GACGCC G[GA]^CG[CT]C BsaWI 3389 ACCGGT [AT]^CCGG[AT] BspLU11I 3564 ACATGT A^CATGT BsrFI 3389 ACCGGT [GA]^CCGG[CT] BssHII 116 GCGCGC G^CGCGC Bst1107I 2682 GTATAC GTA^TAC BstXI 4412 CCAAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2682 GTATAC GTA^TAC CelII 540 GCTTAGC GC^T[ACGT]AGC Cfr10I 3389 ACCGGT [GA]^CCGG[CT] DraII 15 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 6004 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 1024 CCTAATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 15 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 1828 ATGCAT ATGCA^T HgaI 130 GACGC GACGC(5/10) Hin1I 130 GACGCC G[GA]^CG[CT]C HincII 5887 GTTAAC GT[CT]^[GA]AC HindII 5887 GTTAAC GT[CT]^[GA]AC HpaI 5887 GTTAAC GTT^AAC MamI 2440 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 910 GCTAGC G^CTAGC NsiI 1828 ATGCAT ATGCA^T PflFI 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3389 ACCGGT A^CCGGT PmaCI 4963 CACGTG CAC^GTG PmlI 4963 CACGTG CAC^GTG SacI 27 GAGCTC GAGCT^C SapI 90 GCTCTTC GCTCTTC(1/4) SexAI 1655 ACCTGGT A^CC[AT]GGT SfiI 60 GGCCAGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 4528 ACTAGT A^CTAGT SwaI 5012 ATTTAAAT ATTT^AAAT Tth111I 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 2682 GTATAC GT^[AC][GT]AC 3706 GTAGAC AflIII 3564 ACATGT A^C[GA][CT]GT 4964 ACGTGT Alw44I 4407 GTGCAC G^TGCAC 5563 GTGCAC AocI 3435 CCTGAGG CC^T[ACGT]AGG 4309 CCTAAGG ApaLI 4407 GTGCAC G^TGCAC 5563 GTGCAC BalI 3245 TGGCCA TGG^CCA 4456 TGGCCA BciVI 4314 GGATAC GTATCC(6/5) 2667 GTATCC BsmBI 128 GAGACG CGTCTC(1/5) 3258 GAGACG BsmI 2002 GAATGC GAATGC(1/-1) 4256 GCATTC BspMI 3317 ACCTGC ACCTGC(4/8) 5455 GCAGGT BstAPI 835 GCACGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6003 GCACGCAGTGC Bsu36I 3435 CCTGAGG CC^T[ACGT]AGG 4309 CCTAAGG DsaI 2805 CCATGG C^C[GA][CT]GG 5236 CCATGG Eco81I 3435 CCTGAGG CC^T[ACGT]AGG 4309 CCTAAGG HaeII 1597 GGCGCT [GA]GCGC^[CT] 3615 GGCGCT HindIII 993 AAGCTT A^AGCTT 4603 AAGCTT MluNI 3245 TGGCCA TGG^CCA 4456 TGGCCA MscI 3245 TGGCCA TGG^CCA 4456 TGGCCA NcoI 2805 CCATGG C^CATGG 5236 CCATGG NdeI 4120 CATATG CA^TATG 4490 CATATG PflMI 1297 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1912 CCAGTCCTTGG PvuII 331 CAGCTG CAG^CTG 1205 CAGCTG Van91I 1297 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1912 CCAGTCCTTGG XbaI 3569 TCTAGA T^CTAGA 4300 TCTAGA XcmI 5216 CCAGTTAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5236 CCATGGATGACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshAI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI