##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AhdI        Aor51HI     ApaI        Asp718I     AspEI       
AvaI        AviII       AvrII       BbeI        BcgI        
BfrI        BlnI        BseAI       BsiEI       BsiWI       
BsoBI       BspDI       BspEI       BssSI       BstBI       
BstEII      BstPI       ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       FseI        FspI        KasI        
KpnI        KspI        MluI        MroI        NaeI        
NarI        NgoMIV      NotI        NruI        NspV        
PacI        PaeR7I      PmeI        PpuMI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    StuI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcyI          130  GACGCC               G[GA]^CG[CT]C
AgeI         3389  ACCGGT               A^CCGGT
AscI          115  GGCGCGCC             GG^CGCGCC
AspI         1654  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         493  GGATCC               G^GATCC
BanI         5249  GGTGCC               G^G[CT][GA]CC
BanII          27  GAGCTC               G[GA]GC[CT]^C
BbrPI        4963  CACGTG               CAC^GTG
BbvCI        2764  CCTCAGC              CCTCAGC(-5/-2)
BglI           61  GCCAGCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3055  AGATCT               A^GATCT
BlpI          540  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      540  GCTTAGC              GC^T[ACGT]AGC
BsaAI        4963  CACGTG               [CT]AC^GT[GA]
BsaBI        2440  GATAAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         130  GACGCC               G[GA]^CG[CT]C
BsaWI        3389  ACCGGT               [AT]^CCGG[AT]
BspLU11I     3564  ACATGT               A^CATGT
BsrFI        3389  ACCGGT               [GA]^CCGG[CT]
BssHII        116  GCGCGC               G^CGCGC
Bst1107I     2682  GTATAC               GTA^TAC
BstXI        4412  CCAAAGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2682  GTATAC               GTA^TAC
CelII         540  GCTTAGC              GC^T[ACGT]AGC
Cfr10I       3389  ACCGGT               [GA]^CCGG[CT]
DraII          15  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII       6004  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        1024  CCTAATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       15  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I      1828  ATGCAT               ATGCA^T
HgaI          130  GACGC                GACGC(5/10)
Hin1I         130  GACGCC               G[GA]^CG[CT]C
HincII       5887  GTTAAC               GT[CT]^[GA]AC
HindII       5887  GTTAAC               GT[CT]^[GA]AC
HpaI         5887  GTTAAC               GTT^AAC
MamI         2440  GATAAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI          910  GCTAGC               G^CTAGC
NsiI         1828  ATGCAT               ATGCA^T
PflFI        1654  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3389  ACCGGT               A^CCGGT
PmaCI        4963  CACGTG               CAC^GTG
PmlI         4963  CACGTG               CAC^GTG
SacI           27  GAGCTC               GAGCT^C
SapI           90  GCTCTTC              GCTCTTC(1/4)
SexAI        1655  ACCTGGT              A^CC[AT]GGT
SfiI           60  GGCCAGCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         4528  ACTAGT               A^CTAGT
SwaI         5012  ATTTAAAT             ATTT^AAAT
Tth111I      1654  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         2682  GTATAC               GT^[AC][GT]AC
             3706  GTAGAC              
AflIII       3564  ACATGT               A^C[GA][CT]GT
             4964  ACGTGT              
Alw44I       4407  GTGCAC               G^TGCAC
             5563  GTGCAC              
AocI         3435  CCTGAGG              CC^T[ACGT]AGG
             4309  CCTAAGG             
ApaLI        4407  GTGCAC               G^TGCAC
             5563  GTGCAC              
BalI         3245  TGGCCA               TGG^CCA
             4456  TGGCCA              
BciVI        4314  GGATAC               GTATCC(6/5)
             2667  GTATCC              
BsmBI         128  GAGACG               CGTCTC(1/5)
             3258  GAGACG              
BsmI         2002  GAATGC               GAATGC(1/-1)
             4256  GCATTC              
BspMI        3317  ACCTGC               ACCTGC(4/8)
             5455  GCAGGT              
BstAPI        835  GCACGTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6003  GCACGCAGTGC         
Bsu36I       3435  CCTGAGG              CC^T[ACGT]AGG
             4309  CCTAAGG             
DsaI         2805  CCATGG               C^C[GA][CT]GG
             5236  CCATGG              
Eco81I       3435  CCTGAGG              CC^T[ACGT]AGG
             4309  CCTAAGG             
HaeII        1597  GGCGCT               [GA]GCGC^[CT]
             3615  GGCGCT              
HindIII       993  AAGCTT               A^AGCTT
             4603  AAGCTT              
MluNI        3245  TGGCCA               TGG^CCA
             4456  TGGCCA              
MscI         3245  TGGCCA               TGG^CCA
             4456  TGGCCA              
NcoI         2805  CCATGG               C^CATGG
             5236  CCATGG              
NdeI         4120  CATATG               CA^TATG
             4490  CATATG              
PflMI        1297  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1912  CCAGTCCTTGG         
PvuII         331  CAGCTG               CAG^CTG
             1205  CAGCTG              
Van91I       1297  CCAAGAATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1912  CCAGTCCTTGG         
XbaI         3569  TCTAGA               T^CTAGA
             4300  TCTAGA              
XcmI         5216  CCAGTTAGTACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5236  CCATGGATGACTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AspHI       AvaII       BbsI        BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    Bsp1407I    BspHI       BsrBI       BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EaeI        EarI        
EcoRI       EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshAI       PshBI       
PstI        RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI