##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI BbrPI BbvCI BglII BsaAI
BsiWI BspDI BspHI BspLU11I BsrBI
BsrFI BssSI Bst1107I BstBI BstZ17I
Cfr10I ClaI CpoI DraIII Eam1105I
Eco47III EcoT22I FseI HincII HindII
HpaI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfiI SfuI SgrAI SnaBI
Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 4953 GGTACC G^GTACC
AccIII 2186 TCCGGA T^CCGGA
AflIII 1790 ACGCGT A^C[GA][CT]GT
Asp718I 4953 GGTACC G^GTACC
AviII 2416 TGCGCA TGC^GCA
AvrII 1428 CCTAGG C^CTAGG
BalI 4604 TGGCCA TGG^CCA
BamHI 174 GGATCC G^GATCC
BbeI 273 GGCGCC GGCGC^C
BcgI 296 GCAGGCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 403 TGATCA T^GATCA
BlnI 1428 CCTAGG C^CTAGG
BlpI 602 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 602 GCTTAGC GC^T[ACGT]AGC
BsaBI 3983 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1845 GAGACC GGTCTC(1/5)
BseAI 2186 TCCGGA T^CCGGA
BsiEI 364 CGGCCG CG[GA][CT]^CG
BsmBI 2379 GAGACG CGTCTC(1/5)
Bsp1407I 4256 TGTACA T^GTACA
BspEI 2186 TCCGGA T^CCGGA
BsrGI 4256 TGTACA T^GTACA
BssHII 230 GCGCGC G^CGCGC
BstEII 1457 GGTGACC G^GT[ACGT]ACC
BstPI 1457 GGTGACC G^GT[ACGT]ACC
CelII 602 GCTTAGC GC^T[ACGT]AGC
DrdI 936 GACCTTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 364 CGGCCG C^GGCCG
EclXI 364 CGGCCG C^GGCCG
Eco52I 364 CGGCCG C^GGCCG
EcoNI 736 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1457 GGTGACC G^GT[ACGT]ACC
EcoRI 4870 GAATTC G^AATTC
FbaI 403 TGATCA T^GATCA
FspI 2416 TGCGCA TGC^GCA
KasI 273 GGCGCC G^GCGCC
KpnI 4953 GGTACC GGTAC^C
MaeII 1411 ACGT A^CGT
MamI 3983 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1824 CAATTG C^AATTG
MluI 1790 ACGCGT A^CGCGT
MluNI 4604 TGGCCA TGG^CCA
MroI 2186 TCCGGA T^CCGGA
MscI 4604 TGGCCA TGG^CCA
MunI 1824 CAATTG C^AATTG
NarI 273 GGCGCC GG^CGCC
NcoI 1438 CCATGG C^CATGG
NheI 4172 GCTAGC G^CTAGC
PaeR7I 1685 CTCGAG C^TCGAG
PflMI 3594 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3707 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 2217 GAGCTC GAGCT^C
SexAI 4031 ACCAGGT A^CC[AT]GGT
SfoI 273 GGCGCC GGC^GCC
SpeI 3446 ACTAGT A^CTAGT
SspBI 4256 TGTACA T^GTACA
SspI 3564 AATATT AAT^ATT
Van91I 3594 CCATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4089 TCTAGA T^CTAGA
XhoI 1685 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 273 GGCGCC G[GA]^CG[CT]C
3149 GACGCC
AlwNI 928 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
3013 CAGACTCTG
AocI 1256 CCTCAGG CC^T[ACGT]AGG
4433 CCTAAGG
Asp700I 648 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3422 GAATGTTTTC
AspI 411 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2381 GACGCTGTC
BciVI 885 GTATCC GTATCC(6/5)
1348 GTATCC
BpmI 291 CTGGAG CTGGAG(16/14)
2278 CTGGAG
BsaHI 273 GGCGCC G[GA]^CG[CT]C
3149 GACGCC
BsaWI 1933 ACCGGA [AT]^CCGG[AT]
2186 TCCGGA
BsmI 2012 GAATGC GAATGC(1/-1)
3678 GCATTC
BsrDI 2308 GCAATG GCAATG(2/0)
5167 GCAATG
BstAPI 1985 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3012 GCAGACTCTGC
Bsu36I 1256 CCTCAGG CC^T[ACGT]AGG
4433 CCTAAGG
DraII 2651 GGGGCCT [GA]G^G[ACGT]CC[CT]
3707 AGGTCCT
Eco81I 1256 CCTCAGG CC^T[ACGT]AGG
4433 CCTAAGG
EcoO109I 2651 GGGGCCT [GA]G^G[ACGT]CC[CT]
3707 AGGTCCT
EcoRV 807 GATATC GAT^ATC
829 GATATC
Hin1I 273 GGCGCC G[GA]^CG[CT]C
3149 GACGCC
HindIII 3412 AAGCTT A^AGCTT
4039 AAGCTT
KspI 178 CCGCGG CCGC^GG
251 CCGCGG
PflFI 411 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2381 GACGCTGTC
PstI 1995 CTGCAG CTGCA^G
4661 CTGCAG
PvuII 1909 CAGCTG CAG^CTG
4354 CAGCTG
SacII 178 CCGCGG CCGC^GG
251 CCGCGG
ScaI 4639 AGTACT AGT^ACT
4729 AGTACT
SphI 223 GCATGC GCATG^C
553 GCATGC
Tth111I 411 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2381 GACGCTGTC
XcmI 1802 CCAGTCCCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2739 CCACAGAAATCGTGG
XmnI 648 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3422 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI Alw44I AlwI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BfrI
BglI BmyI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI