##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BcgI BlnI BlpI Bpu1102I
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BssHII Bst1107I
BstEII BstPI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FauI
FseI FspI KpnI MfeI MluI
MroI MunI NdeI NheI NotI
NruI PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI SexAI SfiI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 926 CTTAAG C^TTAAG
BbrPI 575 CACGTG CAC^GTG
BbvCI 3167 CCTCAGC CCTCAGC(-5/-2)
BciVI 287 GTATCC GTATCC(6/5)
BfrI 926 CTTAAG C^TTAAG
BglI 4385 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1555 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 740 GAGACG CGTCTC(1/5)
Bsp1407I 1203 TGTACA T^GTACA
BsrFI 2571 GCCGGC [GA]^CCGG[CT]
BsrGI 1203 TGTACA T^GTACA
BstBI 2055 TTCGAA TT^CGAA
Cfr10I 2571 GCCGGC [GA]^CCGG[CT]
DraIII 2345 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2386 GAATTC G^AATTC
EcoT22I 4134 ATGCAT ATGCA^T
HpaI 3337 GTTAAC GTT^AAC
KspI 48 CCGCGG CCGC^GG
MamI 1555 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2571 GCCGGC GCC^GGC
NgoMIV 2571 GCCGGC G^CCGGC
NsiI 4134 ATGCAT ATGCA^T
NspI 4132 GCATGC [GA]CATG^[CT]
NspV 2055 TTCGAA TT^CGAA
PmaCI 575 CACGTG CAC^GTG
PmlI 575 CACGTG CAC^GTG
PpuMI 2493 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 48 CCGCGG CCGC^GG
ScaI 2379 AGTACT AGT^ACT
SfuI 2055 TTCGAA TT^CGAA
SphI 4132 GCATGC GCATG^C
SspBI 1203 TGTACA T^GTACA
SspI 3368 AATATT AAT^ATT
XbaI 2470 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 549 GGGCCC GGGCC^C
2335 GGGCCC
Asp700I 1507 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3117 GAAGTGCTTC
BalI 156 TGGCCA TGG^CCA
4256 TGGCCA
BclI 2928 TGATCA T^GATCA
4835 TGATCA
BsaAI 575 CACGTG [CT]AC^GT[GA]
2150 CACGTA
BspHI 993 TCATGA T^CATGA
3288 TCATGA
BspMI 1590 ACCTGC ACCTGC(4/8)
622 GCAGGT
BsrDI 415 CATTGC GCAATG(2/0)
2403 GCAATG
BssSI 2252 CACGAG CACGAG(-5/-1)
4387 CACGAG
BstAPI 1672 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1870 GCAGTCACTGC
EcoNI 1477 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2614 CCTCCCCAAGG
FbaI 2928 TGATCA T^GATCA
4835 TGATCA
HgaI 2 GCGTC GACGC(5/10)
2115 GCGTC
HindIII 1051 AAGCTT A^AGCTT
3405 AAGCTT
MluNI 156 TGGCCA TGG^CCA
4256 TGGCCA
MscI 156 TGGCCA TGG^CCA
4256 TGGCCA
PaeR7I 849 CTCGAG C^TCGAG
2832 CTCGAG
RcaI 993 TCATGA T^CATGA
3288 TCATGA
XhoI 849 CTCGAG C^TCGAG
2832 CTCGAG
XmnI 1507 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3117 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII