##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AflII AflIII AhdI AscI
AseI AsnI AspEI AspI BcgI
BfrI BglII BsaBI BsiWI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoRV EcoT22I FseI HpaI KspI
MamI MluI NdeI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfuI
SmlI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3068 GACGTC GACGT^C
Acc65I 1300 GGTACC G^GTACC
AccIII 5373 TCCGGA T^CCGGA
AgeI 3214 ACCGGT A^CCGGT
Alw44I 2509 GTGCAC G^TGCAC
Aor51HI 1731 AGCGCT AGC^GCT
ApaLI 2509 GTGCAC G^TGCAC
Asp718I 1300 GGTACC G^GTACC
BbrPI 5481 CACGTG CAC^GTG
BciVI 1810 GTATCC GTATCC(6/5)
BclI 1784 TGATCA T^GATCA
BsaAI 5481 CACGTG [CT]AC^GT[GA]
BseAI 5373 TCCGGA T^CCGGA
BsmI 1517 GCATTC GAATGC(1/-1)
Bsp1407I 831 TGTACA T^GTACA
BspEI 5373 TCCGGA T^CCGGA
BsrGI 831 TGTACA T^GTACA
BstEII 4709 GGTGACC G^GT[ACGT]ACC
BstPI 4709 GGTGACC G^GT[ACGT]ACC
DraIII 2275 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1731 AGCGCT AGC^GCT
EcoO65I 4709 GGTGACC G^GT[ACGT]ACC
FbaI 1784 TGATCA T^GATCA
HincII 1307 GTCAAC GT[CT]^[GA]AC
HindII 1307 GTCAAC GT[CT]^[GA]AC
HindIII 1106 AAGCTT A^AGCTT
KpnI 1300 GGTACC GGTAC^C
MroI 5373 TCCGGA T^CCGGA
NaeI 2915 GCCGGC GCC^GGC
NgoMIV 2915 GCCGGC G^CCGGC
NheI 304 GCTAGC G^CTAGC
NotI 40 GCGGCCGC GC^GGCCGC
PinAI 3214 ACCGGT A^CCGGT
PmaCI 5481 CACGTG CAC^GTG
PmlI 5481 CACGTG CAC^GTG
SexAI 3476 ACCTGGT A^CC[AT]GGT
SfiI 3335 GGCCTGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 3213 CACCGGTG C[GA]^CCGG[CT]G
SphI 1748 GCATGC GCATG^C
SspBI 831 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Asp700I 1956 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3256 GAACCGCTTC
AviII 1745 TGCGCA TGC^GCA
5311 TGCGCA
AvrII 3358 CCTAGG C^CTAGG
5063 CCTAGG
BbvCI 2344 CCTCAGC CCTCAGC(-5/-2)
5302 CCTCAGC
BlnI 3358 CCTAGG C^CTAGG
5063 CCTAGG
BsiEI 41 CGGCCG CG[GA][CT]^CG
3244 CGGCCG
BsmBI 2839 CGTCTC CGTCTC(1/5)
4546 CGTCTC
BsrBI 44 CCGCTC CCGCTC(-3/-3)
3485 CCGCTC
EagI 41 CGGCCG C^GGCCG
3244 CGGCCG
EclXI 41 CGGCCG C^GGCCG
3244 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
3244 CGGCCG
EcoRI 4653 GAATTC G^AATTC
5576 GAATTC
FspI 1745 TGCGCA TGC^GCA
5311 TGCGCA
HgaI 32 GACGC GACGC(5/10)
4249 GCGTC
MfeI 1940 CAATTG C^AATTG
2570 CAATTG
MunI 1940 CAATTG C^AATTG
2570 CAATTG
SapI 4790 GCTCTTC GCTCTTC(1/4)
5097 GCTCTTC
SmaI 732 CCCGGG CCC^GGG
4032 CCCGGG
XmaCI 732 CCCGGG C^CCGGG
4032 CCCGGG
XmaI 732 CCCGGG C^CCGGG
4032 CCCGGG
XmnI 1956 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3256 GAACCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII