##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Aor51HI ApaI AscI Asp700I AspEI
BbrPI BfrI BglII BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrBI BssHII Bst1107I BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI KspI MfeI
MluI MroI MunI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 4103 GGTACC G^GTACC
AccI 3188 GTCTAC GT^[AC][GT]AC
AccII 1045 CGCG CG^CG
AclI 3197 AACGTT AA^CGTT
AflIII 6272 ACATGT A^C[GA][CT]GT
AocI 2256 CCTAAGG CC^T[ACGT]AGG
AseI 2834 ATTAAT AT^TAAT
AsnI 2834 ATTAAT AT^TAAT
Asp718I 4103 GGTACC G^GTACC
BamHI 3807 GGATCC G^GATCC
BbeI 2062 GGCGCC GGCGC^C
BbsI 3826 GAAGAC GAAGAC(2/6)
BciVI 5112 GGATAC GTATCC(6/5)
BclI 5369 TGATCA T^GATCA
BpuAI 3826 GAAGAC GAAGAC(2/6)
BsaAI 243 TACGTG [CT]AC^GT[GA]
BsaBI 4133 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1529 CGTCTC CGTCTC(1/5)
BsmI 1389 GAATGC GAATGC(1/-1)
BspLU11I 6272 ACATGT A^CATGT
BssSI 3358 CTCGTG CACGAG(-5/-1)
BstAPI 2906 GCAACTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3823 TTCGAA TT^CGAA
BstUI 1045 CGCG CG^CG
Bsu36I 2256 CCTAAGG CC^T[ACGT]AGG
DrdI 3600 GACGATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2256 CCTAAGG CC^T[ACGT]AGG
EcoNI 4826 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 5369 TGATCA T^GATCA
HgaI 5565 GACGC GACGC(5/10)
HpaI 996 GTTAAC GTT^AAC
KasI 2062 GGCGCC G^GCGCC
KpnI 4103 GGTACC GGTAC^C
MamI 4133 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1045 CGCG CG^CG
NarI 2062 GGCGCC GG^CGCC
NheI 1469 GCTAGC G^CTAGC
NspV 3823 TTCGAA TT^CGAA
PaeR7I 5074 CTCGAG C^TCGAG
PshAI 5126 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2834 ATTAAT AT^TAAT
Psp1406I 3197 AACGTT AA^CGTT
SapI 2433 GCTCTTC GCTCTTC(1/4)
SfoI 2062 GGCGCC GGC^GCC
SfuI 3823 TTCGAA TT^CGAA
SmaI 1750 CCCGGG CCC^GGG
XhoI 5074 CTCGAG C^TCGAG
XmaCI 1750 CCCGGG C^CCGGG
XmaI 1750 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 2062 GGCGCC G[GA]^CG[CT]C
5565 GACGCC
AviII 844 TGCGCA TGC^GCA
2706 TGCGCA
AvrII 179 CCTAGG C^CTAGG
4718 CCTAGG
BbvCI 233 CCTCAGC CCTCAGC(-5/-2)
1579 CCTCAGC
BcgI 2736 CGAAGATACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5570 CGACTCTTTTGC
BlnI 179 CCTAGG C^CTAGG
4718 CCTAGG
BsaHI 2062 GGCGCC G[GA]^CG[CT]C
5565 GACGCC
Bsp1407I 469 TGTACA T^GTACA
4864 TGTACA
BsrGI 469 TGTACA T^GTACA
4864 TGTACA
BstEII 558 GGTCACC G^GT[ACGT]ACC
2309 GGTGACC
BstPI 558 GGTCACC G^GT[ACGT]ACC
2309 GGTGACC
DraI 2886 TTTAAA TTT^AAA
5165 TTTAAA
EcoO65I 558 GGTCACC G^GT[ACGT]ACC
2309 GGTGACC
EcoRV 4947 GATATC GAT^ATC
5148 GATATC
FspI 844 TGCGCA TGC^GCA
2706 TGCGCA
Hin1I 2062 GGCGCC G[GA]^CG[CT]C
5565 GACGCC
NdeI 1452 CATATG CA^TATG
6150 CATATG
PpuMI 2260 AGGACCT [GA]G^G[AT]CC[CT]
5845 AGGACCT
SexAI 2009 ACCAGGT A^CC[AT]GGT
2305 ACCTGGT
SfiI 1023 GGCCTGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3465 GGCCGATATGGCC
SphI 281 GCATGC GCATG^C
1372 GCATGC
SspBI 469 TGTACA T^GTACA
4864 TGTACA
SspI 690 AATATT AAT^ATT
6008 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII