##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AscI        Asp718I     AviII       AvrII       BamHI       
BbrPI       BcgI        BlnI        BseAI       BsmI        
BspDI       BspEI       BspLU11I    Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraIII      EcoRV       
FseI        FspI        HpaI        KpnI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PmaCI       
PmeI        PmlI        Psp1406I    PvuI        RsrII       
SalI        SbfI        SfuI        SpeI        SphI        
Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI          452  GGCGCC               G[GA]^CG[CT]C
AgeI          139  ACCGGT               A^CCGGT
AhdI         3392  GACTTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2870  GTGCAC               G^TGCAC
AocI         2181  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       118  AGCGCT               AGC^GCT
ApaI         4444  GGGCCC               GGGCC^C
ApaLI        2870  GTGCAC               G^TGCAC
AseI         5888  ATTAAT               AT^TAAT
AsnI         5888  ATTAAT               AT^TAAT
AspEI        3392  GACTTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2784  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI          452  GGCGCC               GGCGC^C
BclI         1379  TGATCA               T^GATCA
BglII        1277  AGATCT               A^GATCT
BsaAI        1438  TACGTA               [CT]AC^GT[GA]
BsaBI         754  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         452  GGCGCC               G[GA]^CG[CT]C
BsaWI         139  ACCGGT               [AT]^CCGG[AT]
BsiEI        2310  CGGCCG               CG[GA][CT]^CG
BsiWI        1510  CGTACG               C^GTACG
BsrBI        2082  GAGCGG               CCGCTC(-3/-3)
BssSI         309  CTCGTG               CACGAG(-5/-1)
Bsu36I       2181  CCTAAGG              CC^T[ACGT]AGG
DrdI         1651  GACATCTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2310  CGGCCG               C^GGCCG
Eam1105I     3392  GACTTCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2310  CGGCCG               C^GGCCG
Eco47III      118  AGCGCT               AGC^GCT
Eco52I       2310  CGGCCG               C^GGCCG
Eco81I       2181  CCTAAGG              CC^T[ACGT]AGG
EcoNI        2875  CCTGGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1379  TGATCA               T^GATCA
HgaI          573  GCGTC                GACGC(5/10)
Hin1I         452  GGCGCC               G[GA]^CG[CT]C
KasI          452  GGCGCC               G^GCGCC
KspI            5  CCGCGG               CCGC^GG
MamI          754  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI          452  GGCGCC               GG^CGCC
NcoI         2908  CCATGG               C^CATGG
PflFI        2784  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4248  CCACTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         139  ACCGGT               A^CCGGT
PshBI        5888  ATTAAT               AT^TAAT
SacII           5  CCGCGG               CCGC^GG
ScaI         2137  AGTACT               AGT^ACT
SexAI        2258  ACCTGGT              A^CC[AT]GGT
SfiI          169  GGCCTCCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          452  GGCGCC               GGC^GCC
SgrAI        2209  CGCCGGCG             C[GA]^CCGG[CT]G
SnaBI        1438  TACGTA               TAC^GTA
Tth111I      2784  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4248  CCACTCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2068  CCAGTAGCATCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI         1013  GTCTAC               GT^[AC][GT]AC
             3716  GTCTAC              
AflII        5444  CTTAAG               C^TTAAG
             5502  CTTAAG              
BfrI         5444  CTTAAG               C^TTAAG
             5502  CTTAAG              
BglI          170  GCCTCCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              420  GCCCCGGCGGC         
BsmBI         574  CGTCTC               CGTCTC(1/5)
             6461  GAGACG              
Bsp1407I     3665  TGTACA               T^GTACA
             5272  TGTACA              
BspHI        1071  TCATGA               T^CATGA
             5541  TCATGA              
BsrGI        3665  TGTACA               T^GTACA
             5272  TGTACA              
BstEII        528  GGTGACC              G^GT[ACGT]ACC
             1536  GGTCACC             
BstPI         528  GGTGACC              G^GT[ACGT]ACC
             1536  GGTCACC             
EcoO65I       528  GGTGACC              G^GT[ACGT]ACC
             1536  GGTCACC             
EcoT22I      3679  ATGCAT               ATGCA^T
             4985  ATGCAT              
HincII        648  GTCAAC               GT[CT]^[GA]AC
             3703  GTTGAC              
HindII        648  GTCAAC               GT[CT]^[GA]AC
             3703  GTTGAC              
HindIII      2558  AAGCTT               A^AGCTT
             6352  AAGCTT              
NaeI         2210  GCCGGC               GCC^GGC
             2312  GCCGGC              
NgoMIV       2210  GCCGGC               G^CCGGC
             2312  GCCGGC              
NsiI         3679  ATGCAT               ATGCA^T
             4985  ATGCAT              
NspI          908  ACATGC               [GA]CATG^[CT]
             3632  ACATGC              
PaeR7I       4411  CTCGAG               C^TCGAG
             6488  CTCGAG              
PpuMI         590  AGGACCC              [GA]G^G[AT]CC[CT]
             6057  GGGTCCT             
PshAI        1530  GACTGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1620  GACACCCGTC          
RcaI         1071  TCATGA               T^CATGA
             5541  TCATGA              
SacI         2127  GAGCTC               GAGCT^C
             3143  GAGCTC              
SmaI           20  CCCGGG               CCC^GGG
             2232  CCCGGG              
SspBI        3665  TGTACA               T^GTACA
             5272  TGTACA              
XbaI         2037  TCTAGA               T^CTAGA
             4630  TCTAGA              
XhoI         4411  CTCGAG               C^TCGAG
             6488  CTCGAG              
XmaCI          20  CCCGGG               C^CCGGG
             2232  CCCGGG              
XmaI           20  CCCGGG               C^CCGGG
             2232  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BciVI       BcnI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssHII      BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI