##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AscI Asp718I AviII AvrII BamHI
BbrPI BcgI BlnI BseAI BsmI
BspDI BspEI BspLU11I Bst1107I BstBI
BstZ17I ClaI CpoI DraIII EcoRV
FseI FspI HpaI KpnI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI SfuI SpeI SphI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 452 GGCGCC G[GA]^CG[CT]C
AgeI 139 ACCGGT A^CCGGT
AhdI 3392 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2870 GTGCAC G^TGCAC
AocI 2181 CCTAAGG CC^T[ACGT]AGG
Aor51HI 118 AGCGCT AGC^GCT
ApaI 4444 GGGCCC GGGCC^C
ApaLI 2870 GTGCAC G^TGCAC
AseI 5888 ATTAAT AT^TAAT
AsnI 5888 ATTAAT AT^TAAT
AspEI 3392 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2784 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 452 GGCGCC GGCGC^C
BclI 1379 TGATCA T^GATCA
BglII 1277 AGATCT A^GATCT
BsaAI 1438 TACGTA [CT]AC^GT[GA]
BsaBI 754 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 452 GGCGCC G[GA]^CG[CT]C
BsaWI 139 ACCGGT [AT]^CCGG[AT]
BsiEI 2310 CGGCCG CG[GA][CT]^CG
BsiWI 1510 CGTACG C^GTACG
BsrBI 2082 GAGCGG CCGCTC(-3/-3)
BssSI 309 CTCGTG CACGAG(-5/-1)
Bsu36I 2181 CCTAAGG CC^T[ACGT]AGG
DrdI 1651 GACATCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2310 CGGCCG C^GGCCG
Eam1105I 3392 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2310 CGGCCG C^GGCCG
Eco47III 118 AGCGCT AGC^GCT
Eco52I 2310 CGGCCG C^GGCCG
Eco81I 2181 CCTAAGG CC^T[ACGT]AGG
EcoNI 2875 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1379 TGATCA T^GATCA
HgaI 573 GCGTC GACGC(5/10)
Hin1I 452 GGCGCC G[GA]^CG[CT]C
KasI 452 GGCGCC G^GCGCC
KspI 5 CCGCGG CCGC^GG
MamI 754 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 452 GGCGCC GG^CGCC
NcoI 2908 CCATGG C^CATGG
PflFI 2784 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4248 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 139 ACCGGT A^CCGGT
PshBI 5888 ATTAAT AT^TAAT
SacII 5 CCGCGG CCGC^GG
ScaI 2137 AGTACT AGT^ACT
SexAI 2258 ACCTGGT A^CC[AT]GGT
SfiI 169 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 452 GGCGCC GGC^GCC
SgrAI 2209 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 1438 TACGTA TAC^GTA
Tth111I 2784 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4248 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2068 CCAGTAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1013 GTCTAC GT^[AC][GT]AC
3716 GTCTAC
AflII 5444 CTTAAG C^TTAAG
5502 CTTAAG
BfrI 5444 CTTAAG C^TTAAG
5502 CTTAAG
BglI 170 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
420 GCCCCGGCGGC
BsmBI 574 CGTCTC CGTCTC(1/5)
6461 GAGACG
Bsp1407I 3665 TGTACA T^GTACA
5272 TGTACA
BspHI 1071 TCATGA T^CATGA
5541 TCATGA
BsrGI 3665 TGTACA T^GTACA
5272 TGTACA
BstEII 528 GGTGACC G^GT[ACGT]ACC
1536 GGTCACC
BstPI 528 GGTGACC G^GT[ACGT]ACC
1536 GGTCACC
EcoO65I 528 GGTGACC G^GT[ACGT]ACC
1536 GGTCACC
EcoT22I 3679 ATGCAT ATGCA^T
4985 ATGCAT
HincII 648 GTCAAC GT[CT]^[GA]AC
3703 GTTGAC
HindII 648 GTCAAC GT[CT]^[GA]AC
3703 GTTGAC
HindIII 2558 AAGCTT A^AGCTT
6352 AAGCTT
NaeI 2210 GCCGGC GCC^GGC
2312 GCCGGC
NgoMIV 2210 GCCGGC G^CCGGC
2312 GCCGGC
NsiI 3679 ATGCAT ATGCA^T
4985 ATGCAT
NspI 908 ACATGC [GA]CATG^[CT]
3632 ACATGC
PaeR7I 4411 CTCGAG C^TCGAG
6488 CTCGAG
PpuMI 590 AGGACCC [GA]G^G[AT]CC[CT]
6057 GGGTCCT
PshAI 1530 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1620 GACACCCGTC
RcaI 1071 TCATGA T^CATGA
5541 TCATGA
SacI 2127 GAGCTC GAGCT^C
3143 GAGCTC
SmaI 20 CCCGGG CCC^GGG
2232 CCCGGG
SspBI 3665 TGTACA T^GTACA
5272 TGTACA
XbaI 2037 TCTAGA T^CTAGA
4630 TCTAGA
XhoI 4411 CTCGAG C^TCGAG
6488 CTCGAG
XmaCI 20 CCCGGG C^CCGGG
2232 CCCGGG
XmaI 20 CCCGGG C^CCGGG
2232 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI