################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AscI Asp718I AviII AvrII BamHI BbrPI BcgI BlnI BseAI BsmI BspDI BspEI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraIII EcoRV FseI FspI HpaI KpnI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SfuI SpeI SphI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AcyI 452 GGCGCC G[GA]^CG[CT]C AgeI 139 ACCGGT A^CCGGT AhdI 3392 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2870 GTGCAC G^TGCAC AocI 2181 CCTAAGG CC^T[ACGT]AGG Aor51HI 118 AGCGCT AGC^GCT ApaI 4444 GGGCCC GGGCC^C ApaLI 2870 GTGCAC G^TGCAC AseI 5888 ATTAAT AT^TAAT AsnI 5888 ATTAAT AT^TAAT AspEI 3392 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2784 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 452 GGCGCC GGCGC^C BclI 1379 TGATCA T^GATCA BglII 1277 AGATCT A^GATCT BsaAI 1438 TACGTA [CT]AC^GT[GA] BsaBI 754 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 452 GGCGCC G[GA]^CG[CT]C BsaWI 139 ACCGGT [AT]^CCGG[AT] BsiEI 2310 CGGCCG CG[GA][CT]^CG BsiWI 1510 CGTACG C^GTACG BsrBI 2082 GAGCGG CCGCTC(-3/-3) BssSI 309 CTCGTG CACGAG(-5/-1) Bsu36I 2181 CCTAAGG CC^T[ACGT]AGG DrdI 1651 GACATCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2310 CGGCCG C^GGCCG Eam1105I 3392 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2310 CGGCCG C^GGCCG Eco47III 118 AGCGCT AGC^GCT Eco52I 2310 CGGCCG C^GGCCG Eco81I 2181 CCTAAGG CC^T[ACGT]AGG EcoNI 2875 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1379 TGATCA T^GATCA HgaI 573 GCGTC GACGC(5/10) Hin1I 452 GGCGCC G[GA]^CG[CT]C KasI 452 GGCGCC G^GCGCC KspI 5 CCGCGG CCGC^GG MamI 754 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 452 GGCGCC GG^CGCC NcoI 2908 CCATGG C^CATGG PflFI 2784 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4248 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 139 ACCGGT A^CCGGT PshBI 5888 ATTAAT AT^TAAT SacII 5 CCGCGG CCGC^GG ScaI 2137 AGTACT AGT^ACT SexAI 2258 ACCTGGT A^CC[AT]GGT SfiI 169 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 452 GGCGCC GGC^GCC SgrAI 2209 CGCCGGCG C[GA]^CCGG[CT]G SnaBI 1438 TACGTA TAC^GTA Tth111I 2784 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4248 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2068 CCAGTAGCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1013 GTCTAC GT^[AC][GT]AC 3716 GTCTAC AflII 5444 CTTAAG C^TTAAG 5502 CTTAAG BfrI 5444 CTTAAG C^TTAAG 5502 CTTAAG BglI 170 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 420 GCCCCGGCGGC BsmBI 574 CGTCTC CGTCTC(1/5) 6461 GAGACG Bsp1407I 3665 TGTACA T^GTACA 5272 TGTACA BspHI 1071 TCATGA T^CATGA 5541 TCATGA BsrGI 3665 TGTACA T^GTACA 5272 TGTACA BstEII 528 GGTGACC G^GT[ACGT]ACC 1536 GGTCACC BstPI 528 GGTGACC G^GT[ACGT]ACC 1536 GGTCACC EcoO65I 528 GGTGACC G^GT[ACGT]ACC 1536 GGTCACC EcoT22I 3679 ATGCAT ATGCA^T 4985 ATGCAT HincII 648 GTCAAC GT[CT]^[GA]AC 3703 GTTGAC HindII 648 GTCAAC GT[CT]^[GA]AC 3703 GTTGAC HindIII 2558 AAGCTT A^AGCTT 6352 AAGCTT NaeI 2210 GCCGGC GCC^GGC 2312 GCCGGC NgoMIV 2210 GCCGGC G^CCGGC 2312 GCCGGC NsiI 3679 ATGCAT ATGCA^T 4985 ATGCAT NspI 908 ACATGC [GA]CATG^[CT] 3632 ACATGC PaeR7I 4411 CTCGAG C^TCGAG 6488 CTCGAG PpuMI 590 AGGACCC [GA]G^G[AT]CC[CT] 6057 GGGTCCT PshAI 1530 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1620 GACACCCGTC RcaI 1071 TCATGA T^CATGA 5541 TCATGA SacI 2127 GAGCTC GAGCT^C 3143 GAGCTC SmaI 20 CCCGGG CCC^GGG 2232 CCCGGG SspBI 3665 TGTACA T^GTACA 5272 TGTACA XbaI 2037 TCTAGA T^CTAGA 4630 TCTAGA XhoI 4411 CTCGAG C^TCGAG 6488 CTCGAG XmaCI 20 CCCGGG C^CCGGG 2232 CCCGGG XmaI 20 CCCGGG C^CCGGG 2232 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI