##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AgeI Aor51HI
AscI Asp700I AspI AviII BbeI
BclI BlpI Bpu1102I BseAI BsiWI
BsmI BspDI BspEI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DrdI
Eco47III EcoT22I FbaI FseI FspI
HindIII KasI MluI MroI NaeI
NarI NdeI NgoMIV NheI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RsrII SacI
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AhdI 3199 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5709 GTGCAC G^TGCAC
ApaLI 5709 GTGCAC G^TGCAC
AseI 3584 ATTAAT AT^TAAT
AsnI 3584 ATTAAT AT^TAAT
AspEI 3199 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3361 CCTAGG C^CTAGG
BamHI 1623 GGATCC G^GATCC
BglII 978 AGATCT A^GATCT
BlnI 3361 CCTAGG C^CTAGG
BsaBI 4764 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3118 CGGCCG CG[GA][CT]^CG
BssSI 5114 CTCGTG CACGAG(-5/-1)
BstEII 2113 GGTCACC G^GT[ACGT]ACC
BstPI 2113 GGTCACC G^GT[ACGT]ACC
EagI 3118 CGGCCG C^GGCCG
Eam1105I 3199 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3118 CGGCCG C^GGCCG
Eco52I 3118 CGGCCG C^GGCCG
EcoO65I 2113 GGTCACC G^GT[ACGT]ACC
EcoRI 4753 GAATTC G^AATTC
EcoRV 2820 GATATC GAT^ATC
KspI 367 CCGCGG CCGC^GG
MamI 4764 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 3117 GCGGCCGC GC^GGCCGC
NruI 7 TCGCGA TCG^CGA
PshAI 4640 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3584 ATTAAT AT^TAAT
SacII 367 CCGCGG CCGC^GG
SpeI 4309 ACTAGT A^CTAGT
SphI 5268 GCATGC GCATG^C
SspI 3754 AATATT AAT^ATT
StuI 1245 AGGCCT AGG^CCT
TaqI 447 TCGA T^CGA
TthHB8I 447 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 663 GGTACC G^GTACC
4392 GGTACC
AccI 4031 GTCTAC GT^[AC][GT]AC
4652 GTAGAC
AcyI 401 GACGCC G[GA]^CG[CT]C
451 GACGCC
ApaI 14 GGGCCC GGGCC^C
3275 GGGCCC
Asp718I 663 GGTACC G^GTACC
4392 GGTACC
BbrPI 614 CACGTG CAC^GTG
2714 CACGTG
BcgI 3139 CGAAGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3547 GCAAGCACGTCG
BciVI 4243 GTATCC GTATCC(6/5)
5766 GTATCC
BglI 2020 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3255 GCCCCTCGGGC
BsaHI 401 GACGCC G[GA]^CG[CT]C
451 GACGCC
BsaWI 4714 ACCGGA [AT]^CCGG[AT]
5547 ACCGGA
BsmBI 3626 CGTCTC CGTCTC(1/5)
449 GAGACG
BsrBI 177 CCGCTC CCGCTC(-3/-3)
194 CCGCTC
BssHII 215 GCGCGC G^CGCGC
239 GCGCGC
Hin1I 401 GACGCC G[GA]^CG[CT]C
451 GACGCC
HpaI 3060 GTTAAC GTT^AAC
4969 GTTAAC
KpnI 663 GGTACC GGTAC^C
4392 GGTACC
MfeI 414 CAATTG C^AATTG
3501 CAATTG
MunI 414 CAATTG C^AATTG
3501 CAATTG
PmaCI 614 CACGTG CAC^GTG
2714 CACGTG
PmlI 614 CACGTG CAC^GTG
2714 CACGTG
ScaI 1254 AGTACT AGT^ACT
1753 AGTACT
SexAI 801 ACCTGGT A^CC[AT]GGT
2117 ACCTGGT
SmaI 226 CCCGGG CCC^GGG
3193 CCCGGG
XmaCI 226 CCCGGG C^CCGGG
3193 CCCGGG
XmaI 226 CCCGGG C^CCGGG
3193 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XbaI XcmI XhoII