##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AccIII AgeI AscI
AseI AsnI Asp718I AviII BamHI
BcgI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
ClaI CpoI EcoNI EcoO65I EcoRV
EcoT22I FseI FspI KpnI KspI
MfeI MluI MroI MunI NcoI
NdeI NruI NsiI PacI PinAI
PmeI PshBI PvuI RcaI RsrII
SacII SfiI SgrAI SnaBI SpeI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 4944 AACGTT AA^CGTT
AflII 211 CTTAAG C^TTAAG
AhdI 4178 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 148 GTGCAC G^TGCAC
AocI 4404 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3779 AGCGCT AGC^GCT
ApaLI 148 GTGCAC G^TGCAC
Asp700I 336 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4178 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2279 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2971 CCTAGG C^CTAGG
BfrI 211 CTTAAG C^TTAAG
BlnI 2971 CCTAGG C^CTAGG
BsaBI 4804 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 2092 TGTACA T^GTACA
BsrBI 3139 GAGCGG CCGCTC(-3/-3)
BsrGI 2092 TGTACA T^GTACA
BstBI 1359 TTCGAA TT^CGAA
Bsu36I 4404 CCTCAGG CC^T[ACGT]AGG
DraI 3996 TTTAAA TTT^AAA
DraIII 3704 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2363 CGGCCG C^GGCCG
Eam1105I 4178 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2363 CGGCCG C^GGCCG
Eco47III 3779 AGCGCT AGC^GCT
Eco52I 2363 CGGCCG C^GGCCG
Eco81I 4404 CCTCAGG CC^T[ACGT]AGG
HgaI 2637 GACGC GACGC(5/10)
HpaI 1074 GTTAAC GTT^AAC
MamI 4804 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 99 GCCGGC GCC^GGC
NgoMIV 99 GCCGGC G^CCGGC
NotI 2362 GCGGCCGC GC^GGCCGC
NspV 1359 TTCGAA TT^CGAA
PaeR7I 2479 CTCGAG C^TCGAG
PflFI 2279 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3369 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4944 AACGTT AA^CGTT
SalI 3366 GTCGAC G^TCGAC
SbfI 3180 CCTGCAGG CCTGCA^GG
ScaI 4369 AGTACT AGT^ACT
SexAI 3252 ACCAGGT A^CC[AT]GGT
SfuI 1359 TTCGAA TT^CGAA
Sse8387I 3180 CCTGCAGG CCTGCA^GG
SspBI 2092 TGTACA T^GTACA
Tth111I 2279 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2479 CTCGAG C^TCGAG
XmnI 336 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
ApaI 1191 GGGCCC GGGCC^C
1412 GGGCCC
BalI 673 TGGCCA TGG^CCA
5089 TGGCCA
BbeI 6 GGCGCC GGCGC^C
629 GGCGCC
BbrPI 172 CACGTG CAC^GTG
2580 CACGTG
BclI 4642 TGATCA T^GATCA
5024 TGATCA
BglII 814 AGATCT A^GATCT
1634 AGATCT
BsiEI 38 CGGTCG CG[GA][CT]^CG
2363 CGGCCG
BsmI 4414 GCATTC GAATGC(1/-1)
4961 GCATTC
BsrDI 1451 CATTGC GCAATG(2/0)
3384 CATTGC
BsrFI 99 GCCGGC [GA]^CCGG[CT]
2752 ACCGGC
Cfr10I 99 GCCGGC [GA]^CCGG[CT]
2752 ACCGGC
DrdI 823 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2337 GACACCAACGTC
DsaI 1408 CCGTGG C^C[GA][CT]GG
2074 CCACGG
EcoRI 4102 GAATTC G^AATTC
4498 GAATTC
FbaI 4642 TGATCA T^GATCA
5024 TGATCA
HincII 1074 GTTAAC GT[CT]^[GA]AC
3366 GTCGAC
HindII 1074 GTTAAC GT[CT]^[GA]AC
3366 GTCGAC
KasI 6 GGCGCC G^GCGCC
629 GGCGCC
MluNI 673 TGGCCA TGG^CCA
5089 TGGCCA
MscI 673 TGGCCA TGG^CCA
5089 TGGCCA
NarI 6 GGCGCC GG^CGCC
629 GGCGCC
NheI 5296 GCTAGC G^CTAGC
5300 GCTAGC
PflMI 1117 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3768 CCAGTGGTTGG
PmaCI 172 CACGTG CAC^GTG
2580 CACGTG
PmlI 172 CACGTG CAC^GTG
2580 CACGTG
SacI 1493 GAGCTC GAGCT^C
1847 GAGCTC
SapI 318 GAAGAGC GCTCTTC(1/4)
1490 GAAGAGC
SfoI 6 GGCGCC GGC^GCC
629 GGCGCC
SmaI 86 CCCGGG CCC^GGG
1318 CCCGGG
Van91I 1117 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3768 CCAGTGGTTGG
XbaI 819 TCTAGA T^CTAGA
3455 TCTAGA
XcmI 59 CCAGGGACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
638 CCAGTGCCAGGCTGG
XmaCI 86 CCCGGG C^CCGGG
1318 CCCGGG
XmaI 86 CCCGGG C^CCGGG
1318 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII