################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AccIII AgeI AscI AseI AsnI Asp718I AviII BamHI BcgI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstEII BstPI BstXI BstZ17I ClaI CpoI EcoNI EcoO65I EcoRV EcoT22I FseI FspI KpnI KspI MfeI MluI MroI MunI NcoI NdeI NruI NsiI PacI PinAI PmeI PshBI PvuI RcaI RsrII SacII SfiI SgrAI SnaBI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AclI 4944 AACGTT AA^CGTT AflII 211 CTTAAG C^TTAAG AhdI 4178 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 148 GTGCAC G^TGCAC AocI 4404 CCTCAGG CC^T[ACGT]AGG Aor51HI 3779 AGCGCT AGC^GCT ApaLI 148 GTGCAC G^TGCAC Asp700I 336 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4178 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2279 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2971 CCTAGG C^CTAGG BfrI 211 CTTAAG C^TTAAG BlnI 2971 CCTAGG C^CTAGG BsaBI 4804 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 2092 TGTACA T^GTACA BsrBI 3139 GAGCGG CCGCTC(-3/-3) BsrGI 2092 TGTACA T^GTACA BstBI 1359 TTCGAA TT^CGAA Bsu36I 4404 CCTCAGG CC^T[ACGT]AGG DraI 3996 TTTAAA TTT^AAA DraIII 3704 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2363 CGGCCG C^GGCCG Eam1105I 4178 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2363 CGGCCG C^GGCCG Eco47III 3779 AGCGCT AGC^GCT Eco52I 2363 CGGCCG C^GGCCG Eco81I 4404 CCTCAGG CC^T[ACGT]AGG HgaI 2637 GACGC GACGC(5/10) HpaI 1074 GTTAAC GTT^AAC MamI 4804 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 99 GCCGGC GCC^GGC NgoMIV 99 GCCGGC G^CCGGC NotI 2362 GCGGCCGC GC^GGCCGC NspV 1359 TTCGAA TT^CGAA PaeR7I 2479 CTCGAG C^TCGAG PflFI 2279 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3369 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 4944 AACGTT AA^CGTT SalI 3366 GTCGAC G^TCGAC SbfI 3180 CCTGCAGG CCTGCA^GG ScaI 4369 AGTACT AGT^ACT SexAI 3252 ACCAGGT A^CC[AT]GGT SfuI 1359 TTCGAA TT^CGAA Sse8387I 3180 CCTGCAGG CCTGCA^GG SspBI 2092 TGTACA T^GTACA Tth111I 2279 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2479 CTCGAG C^TCGAG XmnI 336 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### ApaI 1191 GGGCCC GGGCC^C 1412 GGGCCC BalI 673 TGGCCA TGG^CCA 5089 TGGCCA BbeI 6 GGCGCC GGCGC^C 629 GGCGCC BbrPI 172 CACGTG CAC^GTG 2580 CACGTG BclI 4642 TGATCA T^GATCA 5024 TGATCA BglII 814 AGATCT A^GATCT 1634 AGATCT BsiEI 38 CGGTCG CG[GA][CT]^CG 2363 CGGCCG BsmI 4414 GCATTC GAATGC(1/-1) 4961 GCATTC BsrDI 1451 CATTGC GCAATG(2/0) 3384 CATTGC BsrFI 99 GCCGGC [GA]^CCGG[CT] 2752 ACCGGC Cfr10I 99 GCCGGC [GA]^CCGG[CT] 2752 ACCGGC DrdI 823 GACTCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2337 GACACCAACGTC DsaI 1408 CCGTGG C^C[GA][CT]GG 2074 CCACGG EcoRI 4102 GAATTC G^AATTC 4498 GAATTC FbaI 4642 TGATCA T^GATCA 5024 TGATCA HincII 1074 GTTAAC GT[CT]^[GA]AC 3366 GTCGAC HindII 1074 GTTAAC GT[CT]^[GA]AC 3366 GTCGAC KasI 6 GGCGCC G^GCGCC 629 GGCGCC MluNI 673 TGGCCA TGG^CCA 5089 TGGCCA MscI 673 TGGCCA TGG^CCA 5089 TGGCCA NarI 6 GGCGCC GG^CGCC 629 GGCGCC NheI 5296 GCTAGC G^CTAGC 5300 GCTAGC PflMI 1117 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3768 CCAGTGGTTGG PmaCI 172 CACGTG CAC^GTG 2580 CACGTG PmlI 172 CACGTG CAC^GTG 2580 CACGTG SacI 1493 GAGCTC GAGCT^C 1847 GAGCTC SapI 318 GAAGAGC GCTCTTC(1/4) 1490 GAAGAGC SfoI 6 GGCGCC GGC^GCC 629 GGCGCC SmaI 86 CCCGGG CCC^GGG 1318 CCCGGG Van91I 1117 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3768 CCAGTGGTTGG XbaI 819 TCTAGA T^CTAGA 3455 TCTAGA XcmI 59 CCAGGGACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 638 CCAGTGCCAGGCTGG XmaCI 86 CCCGGG C^CCGGG 1318 CCCGGG XmaI 86 CCCGGG C^CCGGG 1318 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII