##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        AscI        
AseI        AsnI        Asp718I     AviII       BamHI       
BcgI        BsaWI       BseAI       BsiWI       BsmBI       
BspDI       BspEI       BspHI       BspLU11I    BssHII      
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
ClaI        CpoI        EcoNI       EcoO65I     EcoRV       
EcoT22I     FseI        FspI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NcoI        
NdeI        NruI        NsiI        PacI        PinAI       
PmeI        PshBI       PvuI        RcaI        RsrII       
SacII       SfiI        SgrAI       SnaBI       SpeI        
SspI        SwaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI         4944  AACGTT               AA^CGTT
AflII         211  CTTAAG               C^TTAAG
AhdI         4178  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        148  GTGCAC               G^TGCAC
AocI         4404  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      3779  AGCGCT               AGC^GCT
ApaLI         148  GTGCAC               G^TGCAC
Asp700I       336  GAACGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        4178  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2279  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2971  CCTAGG               C^CTAGG
BfrI          211  CTTAAG               C^TTAAG
BlnI         2971  CCTAGG               C^CTAGG
BsaBI        4804  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I     2092  TGTACA               T^GTACA
BsrBI        3139  GAGCGG               CCGCTC(-3/-3)
BsrGI        2092  TGTACA               T^GTACA
BstBI        1359  TTCGAA               TT^CGAA
Bsu36I       4404  CCTCAGG              CC^T[ACGT]AGG
DraI         3996  TTTAAA               TTT^AAA
DraIII       3704  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2363  CGGCCG               C^GGCCG
Eam1105I     4178  GACCCAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2363  CGGCCG               C^GGCCG
Eco47III     3779  AGCGCT               AGC^GCT
Eco52I       2363  CGGCCG               C^GGCCG
Eco81I       4404  CCTCAGG              CC^T[ACGT]AGG
HgaI         2637  GACGC                GACGC(5/10)
HpaI         1074  GTTAAC               GTT^AAC
MamI         4804  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI           99  GCCGGC               GCC^GGC
NgoMIV         99  GCCGGC               G^CCGGC
NotI         2362  GCGGCCGC             GC^GGCCGC
NspV         1359  TTCGAA               TT^CGAA
PaeR7I       2479  CTCGAG               C^TCGAG
PflFI        2279  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        3369  GACCTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4944  AACGTT               AA^CGTT
SalI         3366  GTCGAC               G^TCGAC
SbfI         3180  CCTGCAGG             CCTGCA^GG
ScaI         4369  AGTACT               AGT^ACT
SexAI        3252  ACCAGGT              A^CC[AT]GGT
SfuI         1359  TTCGAA               TT^CGAA
Sse8387I     3180  CCTGCAGG             CCTGCA^GG
SspBI        2092  TGTACA               T^GTACA
Tth111I      2279  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2479  CTCGAG               C^TCGAG
XmnI          336  GAACGGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

ApaI         1191  GGGCCC               GGGCC^C
             1412  GGGCCC              
BalI          673  TGGCCA               TGG^CCA
             5089  TGGCCA              
BbeI            6  GGCGCC               GGCGC^C
              629  GGCGCC              
BbrPI         172  CACGTG               CAC^GTG
             2580  CACGTG              
BclI         4642  TGATCA               T^GATCA
             5024  TGATCA              
BglII         814  AGATCT               A^GATCT
             1634  AGATCT              
BsiEI          38  CGGTCG               CG[GA][CT]^CG
             2363  CGGCCG              
BsmI         4414  GCATTC               GAATGC(1/-1)
             4961  GCATTC              
BsrDI        1451  CATTGC               GCAATG(2/0)
             3384  CATTGC              
BsrFI          99  GCCGGC               [GA]^CCGG[CT]
             2752  ACCGGC              
Cfr10I         99  GCCGGC               [GA]^CCGG[CT]
             2752  ACCGGC              
DrdI          823  GACTCCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2337  GACACCAACGTC        
DsaI         1408  CCGTGG               C^C[GA][CT]GG
             2074  CCACGG              
EcoRI        4102  GAATTC               G^AATTC
             4498  GAATTC              
FbaI         4642  TGATCA               T^GATCA
             5024  TGATCA              
HincII       1074  GTTAAC               GT[CT]^[GA]AC
             3366  GTCGAC              
HindII       1074  GTTAAC               GT[CT]^[GA]AC
             3366  GTCGAC              
KasI            6  GGCGCC               G^GCGCC
              629  GGCGCC              
MluNI         673  TGGCCA               TGG^CCA
             5089  TGGCCA              
MscI          673  TGGCCA               TGG^CCA
             5089  TGGCCA              
NarI            6  GGCGCC               GG^CGCC
              629  GGCGCC              
NheI         5296  GCTAGC               G^CTAGC
             5300  GCTAGC              
PflMI        1117  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3768  CCAGTGGTTGG         
PmaCI         172  CACGTG               CAC^GTG
             2580  CACGTG              
PmlI          172  CACGTG               CAC^GTG
             2580  CACGTG              
SacI         1493  GAGCTC               GAGCT^C
             1847  GAGCTC              
SapI          318  GAAGAGC              GCTCTTC(1/4)
             1490  GAAGAGC             
SfoI            6  GGCGCC               GGC^GCC
              629  GGCGCC              
SmaI           86  CCCGGG               CCC^GGG
             1318  CCCGGG              
Van91I       1117  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3768  CCAGTGGTTGG         
XbaI          819  TCTAGA               T^CTAGA
             3455  TCTAGA              
XcmI           59  CCAGGGACGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              638  CCAGTGCCAGGCTGG     
XmaCI          86  CCCGGG               C^CCGGG
             1318  CCCGGG              
XmaI           86  CCCGGG               C^CCGGG
             1318  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        AspHI       AvaI        AvaII       BanI        
BanII       BbsI        BbvCI       BbvI        BciVI       
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII