################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AatII AgeI Aor51HI AscI AviII BglII BsiEI BsiWI BsmBI BspDI BspHI BssHII BstBI ClaI CpoI DraI EagI EclXI Eco47III Eco52I FseI FspI HindIII HpaI MfeI MunI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI PvuI RcaI RsrII SalI SbfI SfuI SgrAI SmaI SnaBI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 5269 GGTACC G^GTACC AccIII 5522 TCCGGA T^CCGGA AclI 1517 AACGTT AA^CGTT AflII 3964 CTTAAG C^TTAAG Alw44I 5217 GTGCAC G^TGCAC ApaLI 5217 GTGCAC G^TGCAC AseI 6498 ATTAAT AT^TAAT AsnI 6498 ATTAAT AT^TAAT Asp718I 5269 GGTACC G^GTACC AspI 4556 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1083 GGATCC G^GATCC BbeI 1375 GGCGCC GGCGC^C BciVI 890 GGATAC GTATCC(6/5) BfrI 3964 CTTAAG C^TTAAG BsaBI 583 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4963 GGTCTC GGTCTC(1/5) BseAI 5522 TCCGGA T^CCGGA BspEI 5522 TCCGGA T^CCGGA BsrDI 1279 CATTGC GCAATG(2/0) Bst1107I 4701 GTATAC GTA^TAC BstEII 3460 GGTTACC G^GT[ACGT]ACC BstPI 3460 GGTTACC G^GT[ACGT]ACC BstZ17I 4701 GTATAC GTA^TAC DrdI 5641 GACATCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3460 GGTTACC G^GT[ACGT]ACC EcoT22I 1962 ATGCAT ATGCA^T KasI 1375 GGCGCC G^GCGCC KpnI 5269 GGTACC GGTAC^C KspI 1851 CCGCGG CCGC^GG MamI 583 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 268 ACGCGT A^CGCGT MroI 5522 TCCGGA T^CCGGA NaeI 720 GCCGGC GCC^GGC NarI 1375 GGCGCC GG^CGCC NgoMIV 720 GCCGGC G^CCGGC NsiI 1962 ATGCAT ATGCA^T PflFI 4556 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 6498 ATTAAT AT^TAAT Psp1406I 1517 AACGTT AA^CGTT SacII 1851 CCGCGG CCGC^GG SapI 2581 GAAGAGC GCTCTTC(1/4) ScaI 399 AGTACT AGT^ACT SexAI 2142 ACCTGGT A^CC[AT]GGT SfiI 4254 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1375 GGCGCC GGC^GCC SpeI 4333 ACTAGT A^CTAGT SspI 5140 AATATT AAT^ATT Tth111I 4556 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 1181 GGCGTC G[GA]^CG[CT]C 1375 GGCGCC AhdI 1860 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5859 GACTGAGGGTC AspEI 1860 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5859 GACTGAGGGTC AvaI 2652 CTCGGG C^[CT]CG[GA]G 5952 CCCGAG BbrPI 5038 CACGTG CAC^GTG 6057 CACGTG BbvCI 6201 CCTCAGC CCTCAGC(-5/-2) 4230 GCTGAGG BcgI 1273 CGAGATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4797 CGATAACACTGC BclI 4350 TGATCA T^GATCA 5237 TGATCA BsaHI 1181 GGCGTC G[GA]^CG[CT]C 1375 GGCGCC BsaWI 3835 TCCGGT [AT]^CCGG[AT] 5522 TCCGGA BsoBI 2652 CTCGGG C^[CT]CG[GA]G 5952 CCCGAG BspLU11I 358 ACATGT A^CATGT 1449 ACATGT BsrFI 720 GCCGGC [GA]^CCGG[CT] 1653 ACCGGC BssSI 2884 CACGAG CACGAG(-5/-1) 6446 CTCGTG Cfr10I 720 GCCGGC [GA]^CCGG[CT] 1653 ACCGGC DraIII 474 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 2959 CACAGTGTG Eam1105I 1860 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5859 GACTGAGGGTC EcoNI 2993 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4436 CCTTGACAAGG EcoRI 1629 GAATTC G^AATTC 2647 GAATTC EcoRV 53 GATATC GAT^ATC 3696 GATATC FbaI 4350 TGATCA T^GATCA 5237 TGATCA Hin1I 1181 GGCGTC G[GA]^CG[CT]C 1375 GGCGCC NdeI 3344 CATATG CA^TATG 6419 CATATG PmaCI 5038 CACGTG CAC^GTG 6057 CACGTG PmlI 5038 CACGTG CAC^GTG 6057 CACGTG StuI 4462 AGGCCT AGG^CCT 5669 AGGCCT XbaI 1738 TCTAGA T^CTAGA 3450 TCTAGA XcmI 510 CCATCTGCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4859 CCACCCAAGGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaII AvrII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspMI BsrBI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI