##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AgeI Aor51HI AscI AviII
BglII BsiEI BsiWI BsmBI BspDI
BspHI BssHII BstBI ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
FseI FspI HindIII HpaI MfeI
MunI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PvuI RcaI RsrII SalI SbfI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 5269 GGTACC G^GTACC
AccIII 5522 TCCGGA T^CCGGA
AclI 1517 AACGTT AA^CGTT
AflII 3964 CTTAAG C^TTAAG
Alw44I 5217 GTGCAC G^TGCAC
ApaLI 5217 GTGCAC G^TGCAC
AseI 6498 ATTAAT AT^TAAT
AsnI 6498 ATTAAT AT^TAAT
Asp718I 5269 GGTACC G^GTACC
AspI 4556 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1083 GGATCC G^GATCC
BbeI 1375 GGCGCC GGCGC^C
BciVI 890 GGATAC GTATCC(6/5)
BfrI 3964 CTTAAG C^TTAAG
BsaBI 583 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4963 GGTCTC GGTCTC(1/5)
BseAI 5522 TCCGGA T^CCGGA
BspEI 5522 TCCGGA T^CCGGA
BsrDI 1279 CATTGC GCAATG(2/0)
Bst1107I 4701 GTATAC GTA^TAC
BstEII 3460 GGTTACC G^GT[ACGT]ACC
BstPI 3460 GGTTACC G^GT[ACGT]ACC
BstZ17I 4701 GTATAC GTA^TAC
DrdI 5641 GACATCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3460 GGTTACC G^GT[ACGT]ACC
EcoT22I 1962 ATGCAT ATGCA^T
KasI 1375 GGCGCC G^GCGCC
KpnI 5269 GGTACC GGTAC^C
KspI 1851 CCGCGG CCGC^GG
MamI 583 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 268 ACGCGT A^CGCGT
MroI 5522 TCCGGA T^CCGGA
NaeI 720 GCCGGC GCC^GGC
NarI 1375 GGCGCC GG^CGCC
NgoMIV 720 GCCGGC G^CCGGC
NsiI 1962 ATGCAT ATGCA^T
PflFI 4556 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 6498 ATTAAT AT^TAAT
Psp1406I 1517 AACGTT AA^CGTT
SacII 1851 CCGCGG CCGC^GG
SapI 2581 GAAGAGC GCTCTTC(1/4)
ScaI 399 AGTACT AGT^ACT
SexAI 2142 ACCTGGT A^CC[AT]GGT
SfiI 4254 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1375 GGCGCC GGC^GCC
SpeI 4333 ACTAGT A^CTAGT
SspI 5140 AATATT AAT^ATT
Tth111I 4556 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1181 GGCGTC G[GA]^CG[CT]C
1375 GGCGCC
AhdI 1860 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5859 GACTGAGGGTC
AspEI 1860 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5859 GACTGAGGGTC
AvaI 2652 CTCGGG C^[CT]CG[GA]G
5952 CCCGAG
BbrPI 5038 CACGTG CAC^GTG
6057 CACGTG
BbvCI 6201 CCTCAGC CCTCAGC(-5/-2)
4230 GCTGAGG
BcgI 1273 CGAGATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4797 CGATAACACTGC
BclI 4350 TGATCA T^GATCA
5237 TGATCA
BsaHI 1181 GGCGTC G[GA]^CG[CT]C
1375 GGCGCC
BsaWI 3835 TCCGGT [AT]^CCGG[AT]
5522 TCCGGA
BsoBI 2652 CTCGGG C^[CT]CG[GA]G
5952 CCCGAG
BspLU11I 358 ACATGT A^CATGT
1449 ACATGT
BsrFI 720 GCCGGC [GA]^CCGG[CT]
1653 ACCGGC
BssSI 2884 CACGAG CACGAG(-5/-1)
6446 CTCGTG
Cfr10I 720 GCCGGC [GA]^CCGG[CT]
1653 ACCGGC
DraIII 474 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2959 CACAGTGTG
Eam1105I 1860 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5859 GACTGAGGGTC
EcoNI 2993 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4436 CCTTGACAAGG
EcoRI 1629 GAATTC G^AATTC
2647 GAATTC
EcoRV 53 GATATC GAT^ATC
3696 GATATC
FbaI 4350 TGATCA T^GATCA
5237 TGATCA
Hin1I 1181 GGCGTC G[GA]^CG[CT]C
1375 GGCGCC
NdeI 3344 CATATG CA^TATG
6419 CATATG
PmaCI 5038 CACGTG CAC^GTG
6057 CACGTG
PmlI 5038 CACGTG CAC^GTG
6057 CACGTG
StuI 4462 AGGCCT AGG^CCT
5669 AGGCCT
XbaI 1738 TCTAGA T^CTAGA
3450 TCTAGA
XcmI 510 CCATCTGCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4859 CCACCCAAGGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrBI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI