################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccIII AclI AgeI AhdI AocI AscI AseI AsnI AspEI AviII BalI BbeI BcgI BclI BglII BsaWI BseAI BsiWI BspDI BspEI BssHII BstAPI BstBI Bsu36I ClaI CpoI DraIII DrdI Eam1105I Eco81I EcoRV EcoT22I FbaI FspI HindIII KasI KspI MfeI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 3550 GGTACC G^GTACC Asp718I 3550 GGTACC G^GTACC AspI 2810 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 5191 GGATCC G^GATCC BbrPI 4362 CACGTG CAC^GTG BciVI 4757 GTATCC GTATCC(6/5) BsmBI 3338 CGTCTC CGTCTC(1/5) Bsp1407I 4965 TGTACA T^GTACA BspHI 3435 TCATGA T^CATGA BspLU11I 5077 ACATGT A^CATGT BsrDI 1235 CATTGC GCAATG(2/0) BsrGI 4965 TGTACA T^GTACA Bst1107I 4946 GTATAC GTA^TAC BstZ17I 4946 GTATAC GTA^TAC DraI 4574 TTTAAA TTT^AAA EcoRI 863 GAATTC G^AATTC FseI 3559 GGCCGGCC GGCCGG^CC HpaI 5184 GTTAAC GTT^AAC KpnI 3550 GGTACC GGTAC^C NaeI 3560 GCCGGC GCC^GGC NgoMIV 3560 GCCGGC G^CCGGC PflFI 2810 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4362 CACGTG CAC^GTG PmlI 4362 CACGTG CAC^GTG RcaI 3435 TCATGA T^CATGA ScaI 1184 AGTACT AGT^ACT SexAI 256 ACCTGGT A^CC[AT]GGT SgrAI 3932 CACCGGCG C[GA]^CCGG[CT]G SspBI 4965 TGTACA T^GTACA Tth111I 2810 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 2414 CGCG CG^CG 2642 CGCG AflII 66 CTTAAG C^TTAAG 1407 CTTAAG Alw44I 898 GTGCAC G^TGCAC 4359 GTGCAC Aor51HI 90 AGCGCT AGC^GCT 890 AGCGCT ApaLI 898 GTGCAC G^TGCAC 4359 GTGCAC Asp700I 1937 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3832 GAAGGGCTTC BfrI 66 CTTAAG C^TTAAG 1407 CTTAAG BsaAI 2339 TACGTG [CT]AC^GT[GA] 4362 CACGTG BsaBI 2581 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3144 GATGTCAATC BsiEI 109 CGGCCG CG[GA][CT]^CG 2823 CGGCCG BsmI 4318 GAATGC GAATGC(1/-1) 1941 GCATTC BsrBI 1197 GAGCGG CCGCTC(-3/-3) 1212 GAGCGG BstEII 593 GGTCACC G^GT[ACGT]ACC 2946 GGTCACC BstPI 593 GGTCACC G^GT[ACGT]ACC 2946 GGTCACC BstUI 2414 CGCG CG^CG 2642 CGCG EagI 109 CGGCCG C^GGCCG 2823 CGGCCG EclXI 109 CGGCCG C^GGCCG 2823 CGGCCG Eco47III 90 AGCGCT AGC^GCT 890 AGCGCT Eco52I 109 CGGCCG C^GGCCG 2823 CGGCCG EcoO65I 593 GGTCACC G^GT[ACGT]ACC 2946 GGTCACC MamI 2581 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3144 GATGTCAATC MvnI 2414 CGCG CG^CG 2642 CGCG NcoI 646 CCATGG C^CATGG 5195 CCATGG PshAI 2213 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3901 GACTTCAGTC SmaI 2330 CCCGGG CCC^GGG 3497 CCCGGG StuI 616 AGGCCT AGG^CCT 4722 AGGCCT XmaCI 2330 CCCGGG C^CCGGG 3497 CCCGGG XmaI 2330 CCCGGG C^CCGGG 3497 CCCGGG XmnI 1937 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3832 GAAGGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII AvrII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII