##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI AscI AseI AsnI AspEI
AviII BalI BbeI BcgI BclI
BglII BsaWI BseAI BsiWI BspDI
BspEI BssHII BstAPI BstBI Bsu36I
ClaI CpoI DraIII DrdI Eam1105I
Eco81I EcoRV EcoT22I FbaI FspI
HindIII KasI KspI MfeI MluI
MluNI MroI MscI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 3550 GGTACC G^GTACC
Asp718I 3550 GGTACC G^GTACC
AspI 2810 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5191 GGATCC G^GATCC
BbrPI 4362 CACGTG CAC^GTG
BciVI 4757 GTATCC GTATCC(6/5)
BsmBI 3338 CGTCTC CGTCTC(1/5)
Bsp1407I 4965 TGTACA T^GTACA
BspHI 3435 TCATGA T^CATGA
BspLU11I 5077 ACATGT A^CATGT
BsrDI 1235 CATTGC GCAATG(2/0)
BsrGI 4965 TGTACA T^GTACA
Bst1107I 4946 GTATAC GTA^TAC
BstZ17I 4946 GTATAC GTA^TAC
DraI 4574 TTTAAA TTT^AAA
EcoRI 863 GAATTC G^AATTC
FseI 3559 GGCCGGCC GGCCGG^CC
HpaI 5184 GTTAAC GTT^AAC
KpnI 3550 GGTACC GGTAC^C
NaeI 3560 GCCGGC GCC^GGC
NgoMIV 3560 GCCGGC G^CCGGC
PflFI 2810 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4362 CACGTG CAC^GTG
PmlI 4362 CACGTG CAC^GTG
RcaI 3435 TCATGA T^CATGA
ScaI 1184 AGTACT AGT^ACT
SexAI 256 ACCTGGT A^CC[AT]GGT
SgrAI 3932 CACCGGCG C[GA]^CCGG[CT]G
SspBI 4965 TGTACA T^GTACA
Tth111I 2810 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 2414 CGCG CG^CG
2642 CGCG
AflII 66 CTTAAG C^TTAAG
1407 CTTAAG
Alw44I 898 GTGCAC G^TGCAC
4359 GTGCAC
Aor51HI 90 AGCGCT AGC^GCT
890 AGCGCT
ApaLI 898 GTGCAC G^TGCAC
4359 GTGCAC
Asp700I 1937 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3832 GAAGGGCTTC
BfrI 66 CTTAAG C^TTAAG
1407 CTTAAG
BsaAI 2339 TACGTG [CT]AC^GT[GA]
4362 CACGTG
BsaBI 2581 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3144 GATGTCAATC
BsiEI 109 CGGCCG CG[GA][CT]^CG
2823 CGGCCG
BsmI 4318 GAATGC GAATGC(1/-1)
1941 GCATTC
BsrBI 1197 GAGCGG CCGCTC(-3/-3)
1212 GAGCGG
BstEII 593 GGTCACC G^GT[ACGT]ACC
2946 GGTCACC
BstPI 593 GGTCACC G^GT[ACGT]ACC
2946 GGTCACC
BstUI 2414 CGCG CG^CG
2642 CGCG
EagI 109 CGGCCG C^GGCCG
2823 CGGCCG
EclXI 109 CGGCCG C^GGCCG
2823 CGGCCG
Eco47III 90 AGCGCT AGC^GCT
890 AGCGCT
Eco52I 109 CGGCCG C^GGCCG
2823 CGGCCG
EcoO65I 593 GGTCACC G^GT[ACGT]ACC
2946 GGTCACC
MamI 2581 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3144 GATGTCAATC
MvnI 2414 CGCG CG^CG
2642 CGCG
NcoI 646 CCATGG C^CATGG
5195 CCATGG
PshAI 2213 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3901 GACTTCAGTC
SmaI 2330 CCCGGG CCC^GGG
3497 CCCGGG
StuI 616 AGGCCT AGG^CCT
4722 AGGCCT
XmaCI 2330 CCCGGG C^CCGGG
3497 CCCGGG
XmaI 2330 CCCGGG C^CCGGG
3497 CCCGGG
XmnI 1937 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3832 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII AvrII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII