##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AflIII AgeI ApaI AscI
AseI AsnI AspI AviII BciVI
BclI BsaBI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI EclXI Eco52I EcoRV
FbaI FseI FspI KspI MamI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshAI PshBI
PvuI RcaI RsrII SacII SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2994 GGTACC G^GTACC
AccIII 3060 TCCGGA T^CCGGA
AclI 4750 AACGTT AA^CGTT
Asp718I 2994 GGTACC G^GTACC
BamHI 3171 GGATCC G^GATCC
BbeI 89 GGCGCC GGCGC^C
BbrPI 2035 CACGTG CAC^GTG
BbsI 2939 GTCTTC GAAGAC(2/6)
BbvCI 1080 CCTCAGC CCTCAGC(-5/-2)
BglI 4575 GCCAAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 561 AGATCT A^GATCT
BlpI 2213 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2213 GCTGAGC GC^T[ACGT]AGC
BpuAI 2939 GTCTTC GAAGAC(2/6)
BseAI 3060 TCCGGA T^CCGGA
BspEI 3060 TCCGGA T^CCGGA
BsrDI 1148 GCAATG GCAATG(2/0)
BsrFI 1966 GCCGGT [GA]^CCGG[CT]
BssSI 124 CACGAG CACGAG(-5/-1)
BstEII 400 GGTTACC G^GT[ACGT]ACC
BstPI 400 GGTTACC G^GT[ACGT]ACC
CelII 2213 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1966 GCCGGT [GA]^CCGG[CT]
DraI 2091 TTTAAA TTT^AAA
EcoO65I 400 GGTTACC G^GT[ACGT]ACC
EcoRI 5021 GAATTC G^AATTC
EcoT22I 4780 ATGCAT ATGCA^T
HindIII 3435 AAGCTT A^AGCTT
KasI 89 GGCGCC G^GCGCC
KpnI 2994 GGTACC GGTAC^C
MroI 3060 TCCGGA T^CCGGA
NarI 89 GGCGCC GG^CGCC
NdeI 3146 CATATG CA^TATG
NsiI 4780 ATGCAT ATGCA^T
PaeR7I 3477 CTCGAG C^TCGAG
PmaCI 2035 CACGTG CAC^GTG
PmlI 2035 CACGTG CAC^GTG
PpuMI 4476 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 4750 AACGTT AA^CGTT
SacI 3474 GAGCTC GAGCT^C
SalI 980 GTCGAC G^TCGAC
SexAI 770 ACCTGGT A^CC[AT]GGT
SfoI 89 GGCGCC GGC^GCC
SpeI 2364 ACTAGT A^CTAGT
SphI 2711 GCATGC GCATG^C
StuI 1794 AGGCCT AGG^CCT
XhoI 3477 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 140 CGCG CG^CG
1394 CGCG
AhdI 3537 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4590 GACTCAGAGTC
Alw44I 700 GTGCAC G^TGCAC
4942 GTGCAC
Aor51HI 98 AGCGCT AGC^GCT
923 AGCGCT
ApaLI 700 GTGCAC G^TGCAC
4942 GTGCAC
Asp700I 2883 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5061 GAAAAGATTC
AspEI 3537 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4590 GACTCAGAGTC
BcgI 1148 GCAATGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4782 GCATGTCATTCG
BsaAI 248 TACGTG [CT]AC^GT[GA]
2035 CACGTG
BsaWI 34 TCCGGT [AT]^CCGG[AT]
3060 TCCGGA
BsmI 390 GCATTC GAATGC(1/-1)
420 GCATTC
BstAPI 738 GCAGGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3356 GCAACCTCTGC
BstUI 140 CGCG CG^CG
1394 CGCG
DrdI 1012 GACAGTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1434 GACAGATGAGTC
Eam1105I 3537 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4590 GACTCAGAGTC
Eco47III 98 AGCGCT AGC^GCT
923 AGCGCT
HpaI 358 GTTAAC GTT^AAC
3747 GTTAAC
MvnI 140 CGCG CG^CG
1394 CGCG
NspI 2711 GCATGC [GA]CATG^[CT]
4782 GCATGT
PflMI 873 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4850 CCAGATGGTGG
SapI 838 GAAGAGC GCTCTTC(1/4)
963 GCTCTTC
Van91I 873 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4850 CCAGATGGTGG
XmnI 2883 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5061 GAAAAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII AvrII
BalI BanI BanII BbvI BcnI
BfaI BfrI BlnI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII