################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AviII AvrII BbrPI BcgI BlnI BseAI BsiWI BspDI BspEI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I Eco47III EcoRI EcoRV FseI FspI HindIII HpaI KspI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 4492 GACGTC GACGT^C Alw44I 5127 GTGCAC G^TGCAC ApaLI 5127 GTGCAC G^TGCAC Asp700I 1518 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1004 CCCGAG C^[CT]CG[GA]G BamHI 3620 GGATCC G^GATCC BbeI 1009 GGCGCC GGCGC^C BclI 3376 TGATCA T^GATCA BlpI 6214 GCTGAGC GC^T[ACGT]AGC Bpu1102I 6214 GCTGAGC GC^T[ACGT]AGC BsaAI 542 TACGTG [CT]AC^GT[GA] BsaBI 373 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3049 ACCGGA [AT]^CCGG[AT] BsgI 2102 GTGCAG GTGCAG(16/14) BsiEI 972 CGGCCG CG[GA][CT]^CG BsoBI 1004 CCCGAG C^[CT]CG[GA]G Bsp1407I 1419 TGTACA T^GTACA BsrBI 1213 GAGCGG CCGCTC(-3/-3) BsrFI 2530 GCCGGC [GA]^CCGG[CT] BsrGI 1419 TGTACA T^GTACA CelII 6214 GCTGAGC GC^T[ACGT]AGC Cfr10I 2530 GCCGGC [GA]^CCGG[CT] DraIII 5370 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 230 GACCCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 972 CGGCCG C^GGCCG EclXI 972 CGGCCG C^GGCCG Eco52I 972 CGGCCG C^GGCCG FbaI 3376 TGATCA T^GATCA HaeII 1009 GGCGCC [GA]GCGC^[CT] HincII 1792 GTTGAC GT[CT]^[GA]AC HindII 1792 GTTGAC GT[CT]^[GA]AC KasI 1009 GGCGCC G^GCGCC MamI 373 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2530 GCCGGC GCC^GGC NarI 1009 GGCGCC GG^CGCC NcoI 5625 CCATGG C^CATGG NdeI 2701 CATATG CA^TATG NgoMIV 2530 GCCGGC G^CCGGC PshAI 3977 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 552 CAGCTG CAG^CTG SbfI 184 CCTGCAGG CCTGCA^GG SfoI 1009 GGCGCC GGC^GCC SpeI 3381 ACTAGT A^CTAGT SphI 1227 GCATGC GCATG^C Sse8387I 184 CCTGCAGG CCTGCA^GG SspBI 1419 TGTACA T^GTACA SspI 4688 AATATT AAT^ATT XmnI 1518 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 430 GGTACC G^GTACC 2846 GGTACC AccII 1065 CGCG CG^CG 1899 CGCG Asp718I 430 GGTACC G^GTACC 2846 GGTACC BbvCI 5782 CCTCAGC CCTCAGC(-5/-2) 6085 CCTCAGC BciVI 3681 GGATAC GTATCC(6/5) 573 GTATCC BglI 1001 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5076 GCCCCGAAGGC BglII 822 AGATCT A^GATCT 5979 AGATCT BsmBI 4613 CGTCTC CGTCTC(1/5) 6399 CGTCTC BspHI 4197 TCATGA T^CATGA 5361 TCATGA BspLU11I 3494 ACATGT A^CATGT 4120 ACATGT BsrDI 898 CATTGC GCAATG(2/0) 6318 GCAATG BstAPI 1289 GCACTAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3859 GCAACCTCTGC BstEII 3045 GGTCACC G^GT[ACGT]ACC 4649 GGTTACC BstPI 3045 GGTCACC G^GT[ACGT]ACC 4649 GGTTACC BstUI 1065 CGCG CG^CG 1899 CGCG DsaI 174 CCGTGG C^C[GA][CT]GG 5625 CCATGG EcoNI 5237 CCTCCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5240 CCTACAGGAGG EcoO65I 3045 GGTCACC G^GT[ACGT]ACC 4649 GGTTACC EcoT22I 2698 ATGCAT ATGCA^T 4869 ATGCAT KpnI 430 GGTACC GGTAC^C 2846 GGTACC MspA1I 552 CAGCTG C[AC]G^C[GT]G 969 CAGCGG MvnI 1065 CGCG CG^CG 1899 CGCG NsiI 2698 ATGCAT ATGCA^T 4869 ATGCAT PflMI 2181 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4640 CCAGAGGCTGG RcaI 4197 TCATGA T^CATGA 5361 TCATGA SacI 2107 GAGCTC GAGCT^C 3337 GAGCTC Van91I 2181 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4640 CCAGAGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII