##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AviII AvrII BbrPI BcgI BlnI
BseAI BsiWI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI Eam1105I Eco47III EcoRI EcoRV
FseI FspI HindIII HpaI KspI
MfeI MluI MroI MunI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 4492 GACGTC GACGT^C
Alw44I 5127 GTGCAC G^TGCAC
ApaLI 5127 GTGCAC G^TGCAC
Asp700I 1518 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1004 CCCGAG C^[CT]CG[GA]G
BamHI 3620 GGATCC G^GATCC
BbeI 1009 GGCGCC GGCGC^C
BclI 3376 TGATCA T^GATCA
BlpI 6214 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6214 GCTGAGC GC^T[ACGT]AGC
BsaAI 542 TACGTG [CT]AC^GT[GA]
BsaBI 373 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3049 ACCGGA [AT]^CCGG[AT]
BsgI 2102 GTGCAG GTGCAG(16/14)
BsiEI 972 CGGCCG CG[GA][CT]^CG
BsoBI 1004 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1419 TGTACA T^GTACA
BsrBI 1213 GAGCGG CCGCTC(-3/-3)
BsrFI 2530 GCCGGC [GA]^CCGG[CT]
BsrGI 1419 TGTACA T^GTACA
CelII 6214 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2530 GCCGGC [GA]^CCGG[CT]
DraIII 5370 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 230 GACCCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 972 CGGCCG C^GGCCG
EclXI 972 CGGCCG C^GGCCG
Eco52I 972 CGGCCG C^GGCCG
FbaI 3376 TGATCA T^GATCA
HaeII 1009 GGCGCC [GA]GCGC^[CT]
HincII 1792 GTTGAC GT[CT]^[GA]AC
HindII 1792 GTTGAC GT[CT]^[GA]AC
KasI 1009 GGCGCC G^GCGCC
MamI 373 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2530 GCCGGC GCC^GGC
NarI 1009 GGCGCC GG^CGCC
NcoI 5625 CCATGG C^CATGG
NdeI 2701 CATATG CA^TATG
NgoMIV 2530 GCCGGC G^CCGGC
PshAI 3977 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 552 CAGCTG CAG^CTG
SbfI 184 CCTGCAGG CCTGCA^GG
SfoI 1009 GGCGCC GGC^GCC
SpeI 3381 ACTAGT A^CTAGT
SphI 1227 GCATGC GCATG^C
Sse8387I 184 CCTGCAGG CCTGCA^GG
SspBI 1419 TGTACA T^GTACA
SspI 4688 AATATT AAT^ATT
XmnI 1518 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 430 GGTACC G^GTACC
2846 GGTACC
AccII 1065 CGCG CG^CG
1899 CGCG
Asp718I 430 GGTACC G^GTACC
2846 GGTACC
BbvCI 5782 CCTCAGC CCTCAGC(-5/-2)
6085 CCTCAGC
BciVI 3681 GGATAC GTATCC(6/5)
573 GTATCC
BglI 1001 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5076 GCCCCGAAGGC
BglII 822 AGATCT A^GATCT
5979 AGATCT
BsmBI 4613 CGTCTC CGTCTC(1/5)
6399 CGTCTC
BspHI 4197 TCATGA T^CATGA
5361 TCATGA
BspLU11I 3494 ACATGT A^CATGT
4120 ACATGT
BsrDI 898 CATTGC GCAATG(2/0)
6318 GCAATG
BstAPI 1289 GCACTAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3859 GCAACCTCTGC
BstEII 3045 GGTCACC G^GT[ACGT]ACC
4649 GGTTACC
BstPI 3045 GGTCACC G^GT[ACGT]ACC
4649 GGTTACC
BstUI 1065 CGCG CG^CG
1899 CGCG
DsaI 174 CCGTGG C^C[GA][CT]GG
5625 CCATGG
EcoNI 5237 CCTCCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5240 CCTACAGGAGG
EcoO65I 3045 GGTCACC G^GT[ACGT]ACC
4649 GGTTACC
EcoT22I 2698 ATGCAT ATGCA^T
4869 ATGCAT
KpnI 430 GGTACC GGTAC^C
2846 GGTACC
MspA1I 552 CAGCTG C[AC]G^C[GT]G
969 CAGCGG
MvnI 1065 CGCG CG^CG
1899 CGCG
NsiI 2698 ATGCAT ATGCA^T
4869 ATGCAT
PflMI 2181 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4640 CCAGAGGCTGG
RcaI 4197 TCATGA T^CATGA
5361 TCATGA
SacI 2107 GAGCTC GAGCT^C
3337 GAGCTC
Van91I 2181 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4640 CCAGAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII