##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AgeI AhdI Aor51HI
AscI Asp718I AspEI AviII AvrII
BbrPI BglI BlnI BsaBI BsmBI
BsrBI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I CpoI Eam1105I
Eco47III EcoO65I FseI FspI HgaI
KpnI MamI MfeI MluI MunI
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PvuI RsrII
SacI SalI SbfI SexAI SfiI
SgrAI SmaI SnaBI Sse8387I SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccIII 1413 TCCGGA T^CCGGA
AclI 5071 AACGTT AA^CGTT
AcyI 556 GGCGCC G[GA]^CG[CT]C
AflII 5639 CTTAAG C^TTAAG
Alw44I 3392 GTGCAC G^TGCAC
ApaI 988 GGGCCC GGGCC^C
ApaLI 3392 GTGCAC G^TGCAC
AspI 3720 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 666 CTCGGG C^[CT]CG[GA]G
BalI 4583 TGGCCA TGG^CCA
BbeI 556 GGCGCC GGCGC^C
BbvCI 4591 GCTGAGG CCTCAGC(-5/-2)
BcgI 889 CGATGGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5202 GTATCC GTATCC(6/5)
BcnI 991 CCCGG CC^[GC]GG
BfrI 5639 CTTAAG C^TTAAG
BsaHI 556 GGCGCC G[GA]^CG[CT]C
BsaI 5115 GGTCTC GGTCTC(1/5)
BseAI 1413 TCCGGA T^CCGGA
BsiEI 2362 CGGCCG CG[GA][CT]^CG
BsiWI 511 CGTACG C^GTACG
BsmI 2125 GCATTC GAATGC(1/-1)
BsoBI 666 CTCGGG C^[CT]CG[GA]G
BspDI 1107 ATCGAT AT^CGAT
BspEI 1413 TCCGGA T^CCGGA
BspHI 296 TCATGA T^CATGA
BsrFI 2031 GCCGGC [GA]^CCGG[CT]
BstBI 812 TTCGAA TT^CGAA
BstXI 1075 CCATTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2031 GCCGGC [GA]^CCGG[CT]
ClaI 1107 ATCGAT AT^CGAT
DrdI 1334 GACTGATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2362 CGGCCG C^GGCCG
EclXI 2362 CGGCCG C^GGCCG
Eco52I 2362 CGGCCG C^GGCCG
EcoNI 797 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 556 GGCGCC G[GA]^CG[CT]C
HpaI 681 GTTAAC GTT^AAC
KasI 556 GGCGCC G^GCGCC
KspI 836 CCGCGG CCGC^GG
MluNI 4583 TGGCCA TGG^CCA
MroI 1413 TCCGGA T^CCGGA
MscI 4583 TGGCCA TGG^CCA
NaeI 2031 GCCGGC GCC^GGC
NarI 556 GGCGCC GG^CGCC
NciI 991 CCCGG CC^[GC]GG
NcoI 2490 CCATGG C^CATGG
NgoMIV 2031 GCCGGC G^CCGGC
NheI 2106 GCTAGC G^CTAGC
NspV 812 TTCGAA TT^CGAA
PflFI 3720 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1736 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 4420 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5071 AACGTT AA^CGTT
PvuII 3033 CAGCTG CAG^CTG
RcaI 296 TCATGA T^CATGA
SacII 836 CCGCGG CCGC^GG
ScaI 3145 AGTACT AGT^ACT
SfoI 556 GGCGCC GGC^GCC
SfuI 812 TTCGAA TT^CGAA
Tth111I 3720 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 753 CCAAAACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 837 CGCG CG^CG
5471 CGCG
AocI 983 CCTAAGG CC^T[ACGT]AGG
1254 CCTGAGG
AseI 4100 ATTAAT AT^TAAT
5018 ATTAAT
AsnI 4100 ATTAAT AT^TAAT
5018 ATTAAT
Asp700I 1949 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4262 GAATATATTC
BamHI 1410 GGATCC G^GATCC
5052 GGATCC
BglII 3137 AGATCT A^GATCT
3274 AGATCT
BsaAI 350 CACGTA [CT]AC^GT[GA]
3380 CACGTA
BsaWI 910 TCCGGT [AT]^CCGG[AT]
1413 TCCGGA
BstUI 837 CGCG CG^CG
5471 CGCG
Bsu36I 983 CCTAAGG CC^T[ACGT]AGG
1254 CCTGAGG
Eco81I 983 CCTAAGG CC^T[ACGT]AGG
1254 CCTGAGG
EcoRI 2452 GAATTC G^AATTC
5428 GAATTC
EcoRV 3047 GATATC GAT^ATC
5825 GATATC
EcoT22I 3368 ATGCAT ATGCA^T
3852 ATGCAT
HincII 681 GTTAAC GT[CT]^[GA]AC
3227 GTTGAC
HindII 681 GTTAAC GT[CT]^[GA]AC
3227 GTTGAC
MvnI 837 CGCG CG^CG
5471 CGCG
NsiI 3368 ATGCAT ATGCA^T
3852 ATGCAT
PflMI 70 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1181 CCAGGAAATGG
PshBI 4100 ATTAAT AT^TAAT
5018 ATTAAT
SapI 1481 GAAGAGC GCTCTTC(1/4)
166 GCTCTTC
SmlI 2721 CTCAAG C^T[CT][GA]AG
5639 CTTAAG
SpeI 380 ACTAGT A^CTAGT
3641 ACTAGT
SphI 3430 GCATGC GCATG^C
5363 GCATGC
StuI 805 AGGCCT AGG^CCT
1847 AGGCCT
Van91I 70 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1181 CCAGGAAATGG
XmnI 1949 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4262 GAATATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BclI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII