################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## Acc65I AclI AgeI AscI AseI AsnI Asp718I BamHI BbeI BsiWI BspDI BspHI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI EcoO65I FseI HgaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 558 GACGTC GACGT^C AccIII 1547 TCCGGA T^CCGGA AcyI 558 GACGTC G[GA]^CG[CT]C AflIII 1052 ACATGT A^C[GA][CT]GT AhdI 1577 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3153 CCTCAGG CC^T[ACGT]AGG Aor51HI 3513 AGCGCT AGC^GCT ApaI 2120 GGGCCC GGGCC^C Asp700I 736 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1577 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 937 CTCGAG C^[CT]CG[GA]G AviII 2466 TGCGCA TGC^GCA AvrII 34 CCTAGG C^CTAGG BbrPI 1150 CACGTG CAC^GTG BcgI 2779 GCAGCTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 262 TGATCA T^GATCA BlnI 34 CCTAGG C^CTAGG BsaAI 1150 CACGTG [CT]AC^GT[GA] BsaBI 4229 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 558 GACGTC G[GA]^CG[CT]C BseAI 1547 TCCGGA T^CCGGA BsiEI 4326 CGGCCG CG[GA][CT]^CG BsoBI 937 CTCGAG C^[CT]CG[GA]G BspEI 1547 TCCGGA T^CCGGA BspLU11I 1052 ACATGT A^CATGT BssSI 2159 CTCGTG CACGAG(-5/-1) BstAPI 3243 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3153 CCTCAGG CC^T[ACGT]AGG EagI 4326 CGGCCG C^GGCCG Eam1105I 1577 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 4326 CGGCCG C^GGCCG Eco47III 3513 AGCGCT AGC^GCT Eco52I 4326 CGGCCG C^GGCCG Eco81I 3153 CCTCAGG CC^T[ACGT]AGG EcoNI 1423 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 310 GAATTC G^AATTC EcoRV 2309 GATATC GAT^ATC FbaI 262 TGATCA T^GATCA FspI 2466 TGCGCA TGC^GCA Hin1I 558 GACGTC G[GA]^CG[CT]C HincII 1940 GTTAAC GT[CT]^[GA]AC HindII 1940 GTTAAC GT[CT]^[GA]AC HpaI 1940 GTTAAC GTT^AAC MamI 4229 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1547 TCCGGA T^CCGGA NheI 1357 GCTAGC G^CTAGC PaeR7I 937 CTCGAG C^TCGAG PmaCI 1150 CACGTG CAC^GTG PmlI 1150 CACGTG CAC^GTG PpuMI 2926 GGGTCCT [GA]G^G[AT]CC[CT] PstI 3543 CTGCAG CTGCA^G SacI 134 GAGCTC GAGCT^C SexAI 3563 ACCAGGT A^CC[AT]GGT SfiI 3687 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 3695 AGGCCT AGG^CCT SwaI 3736 ATTTAAAT ATTT^AAAT XbaI 629 TCTAGA T^CTAGA XhoI 937 CTCGAG C^TCGAG XmnI 736 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 4118 CGCG CG^CG 4667 CGCG Alw44I 4997 GTGCAC G^TGCAC 5010 GTGCAC AlwI 4229 GATCC GGATC(4/5) 2388 GGATC ApaLI 4997 GTGCAC G^TGCAC 5010 GTGCAC AspI 2039 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3175 GACTTAGTC BciVI 1768 GGATAC GTATCC(6/5) 3775 GTATCC BlpI 1684 GCTCAGC GC^T[ACGT]AGC 2899 GCTGAGC Bpu1102I 1684 GCTCAGC GC^T[ACGT]AGC 2899 GCTGAGC BsaI 3849 GAGACC GGTCTC(1/5) 2448 GGTCTC BsaWI 1547 TCCGGA [AT]^CCGG[AT] 2730 ACCGGA BsmBI 1964 CGTCTC CGTCTC(1/5) 4302 CGTCTC Bsp1407I 1777 TGTACA T^GTACA 3558 TGTACA BsrDI 903 GCAATG GCAATG(2/0) 2064 GCAATG BsrGI 1777 TGTACA T^GTACA 3558 TGTACA BstUI 4118 CGCG CG^CG 4667 CGCG CelII 1684 GCTCAGC GC^T[ACGT]AGC 2899 GCTGAGC DraIII 1871 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4528 CACTTTGTG DrdI 2105 GACCCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4829 GACTCATCTGTC EcoT22I 587 ATGCAT ATGCA^T 4892 ATGCAT MvnI 4118 CGCG CG^CG 4667 CGCG NsiI 587 ATGCAT ATGCA^T 4892 ATGCAT PflFI 2039 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3175 GACTTAGTC ScaI 722 AGTACT AGT^ACT 3362 AGTACT SspBI 1777 TGTACA T^GTACA 3558 TGTACA Tth111I 2039 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3175 GACTTAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI AlwNI ApoI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BfrI BglI BglII BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PshAI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII