##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

Acc65I      AclI        AgeI        AscI        AseI        
AsnI        Asp718I     BamHI       BbeI        BsiWI       
BspDI       BspHI       BsrBI       BsrFI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        EcoO65I     FseI        
HgaI        KasI        KpnI        KspI        MfeI        
MluI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII         558  GACGTC               GACGT^C
AccIII       1547  TCCGGA               T^CCGGA
AcyI          558  GACGTC               G[GA]^CG[CT]C
AflIII       1052  ACATGT               A^C[GA][CT]GT
AhdI         1577  GACGACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3153  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      3513  AGCGCT               AGC^GCT
ApaI         2120  GGGCCC               GGGCC^C
Asp700I       736  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1577  GACGACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          937  CTCGAG               C^[CT]CG[GA]G
AviII        2466  TGCGCA               TGC^GCA
AvrII          34  CCTAGG               C^CTAGG
BbrPI        1150  CACGTG               CAC^GTG
BcgI         2779  GCAGCTCTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          262  TGATCA               T^GATCA
BlnI           34  CCTAGG               C^CTAGG
BsaAI        1150  CACGTG               [CT]AC^GT[GA]
BsaBI        4229  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         558  GACGTC               G[GA]^CG[CT]C
BseAI        1547  TCCGGA               T^CCGGA
BsiEI        4326  CGGCCG               CG[GA][CT]^CG
BsoBI         937  CTCGAG               C^[CT]CG[GA]G
BspEI        1547  TCCGGA               T^CCGGA
BspLU11I     1052  ACATGT               A^CATGT
BssSI        2159  CTCGTG               CACGAG(-5/-1)
BstAPI       3243  GCACTCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       3153  CCTCAGG              CC^T[ACGT]AGG
EagI         4326  CGGCCG               C^GGCCG
Eam1105I     1577  GACGACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        4326  CGGCCG               C^GGCCG
Eco47III     3513  AGCGCT               AGC^GCT
Eco52I       4326  CGGCCG               C^GGCCG
Eco81I       3153  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1423  CCTGGTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         310  GAATTC               G^AATTC
EcoRV        2309  GATATC               GAT^ATC
FbaI          262  TGATCA               T^GATCA
FspI         2466  TGCGCA               TGC^GCA
Hin1I         558  GACGTC               G[GA]^CG[CT]C
HincII       1940  GTTAAC               GT[CT]^[GA]AC
HindII       1940  GTTAAC               GT[CT]^[GA]AC
HpaI         1940  GTTAAC               GTT^AAC
MamI         4229  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1547  TCCGGA               T^CCGGA
NheI         1357  GCTAGC               G^CTAGC
PaeR7I        937  CTCGAG               C^TCGAG
PmaCI        1150  CACGTG               CAC^GTG
PmlI         1150  CACGTG               CAC^GTG
PpuMI        2926  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         3543  CTGCAG               CTGCA^G
SacI          134  GAGCTC               GAGCT^C
SexAI        3563  ACCAGGT              A^CC[AT]GGT
SfiI         3687  GGCCAGGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI         3695  AGGCCT               AGG^CCT
SwaI         3736  ATTTAAAT             ATTT^AAAT
XbaI          629  TCTAGA               T^CTAGA
XhoI          937  CTCGAG               C^TCGAG
XmnI          736  GAAATACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII        4118  CGCG                 CG^CG
             4667  CGCG                
Alw44I       4997  GTGCAC               G^TGCAC
             5010  GTGCAC              
AlwI         4229  GATCC                GGATC(4/5)
             2388  GGATC               
ApaLI        4997  GTGCAC               G^TGCAC
             5010  GTGCAC              
AspI         2039  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3175  GACTTAGTC           
BciVI        1768  GGATAC               GTATCC(6/5)
             3775  GTATCC              
BlpI         1684  GCTCAGC              GC^T[ACGT]AGC
             2899  GCTGAGC             
Bpu1102I     1684  GCTCAGC              GC^T[ACGT]AGC
             2899  GCTGAGC             
BsaI         3849  GAGACC               GGTCTC(1/5)
             2448  GGTCTC              
BsaWI        1547  TCCGGA               [AT]^CCGG[AT]
             2730  ACCGGA              
BsmBI        1964  CGTCTC               CGTCTC(1/5)
             4302  CGTCTC              
Bsp1407I     1777  TGTACA               T^GTACA
             3558  TGTACA              
BsrDI         903  GCAATG               GCAATG(2/0)
             2064  GCAATG              
BsrGI        1777  TGTACA               T^GTACA
             3558  TGTACA              
BstUI        4118  CGCG                 CG^CG
             4667  CGCG                
CelII        1684  GCTCAGC              GC^T[ACGT]AGC
             2899  GCTGAGC             
DraIII       1871  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4528  CACTTTGTG           
DrdI         2105  GACCCAGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4829  GACTCATCTGTC        
EcoT22I       587  ATGCAT               ATGCA^T
             4892  ATGCAT              
MvnI         4118  CGCG                 CG^CG
             4667  CGCG                
NsiI          587  ATGCAT               ATGCA^T
             4892  ATGCAT              
PflFI        2039  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3175  GACTTAGTC           
ScaI          722  AGTACT               AGT^ACT
             3362  AGTACT              
SspBI        1777  TGTACA               T^GTACA
             3558  TGTACA              
Tth111I      2039  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3175  GACTTAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AluI        AlwNI       ApoI        AspHI       AvaII       
BalI        BanI        BanII       BbsI        BbvCI       
BbvI        BcnI        BfaI        BfrI        BglI        
BglII       BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PshAI       PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII