##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AclI AgeI AscI AseI
AsnI Asp718I BamHI BbeI BsiWI
BspDI BspHI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI EcoO65I FseI
HgaI KasI KpnI KspI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 558 GACGTC GACGT^C
AccIII 1547 TCCGGA T^CCGGA
AcyI 558 GACGTC G[GA]^CG[CT]C
AflIII 1052 ACATGT A^C[GA][CT]GT
AhdI 1577 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3153 CCTCAGG CC^T[ACGT]AGG
Aor51HI 3513 AGCGCT AGC^GCT
ApaI 2120 GGGCCC GGGCC^C
Asp700I 736 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1577 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 937 CTCGAG C^[CT]CG[GA]G
AviII 2466 TGCGCA TGC^GCA
AvrII 34 CCTAGG C^CTAGG
BbrPI 1150 CACGTG CAC^GTG
BcgI 2779 GCAGCTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 262 TGATCA T^GATCA
BlnI 34 CCTAGG C^CTAGG
BsaAI 1150 CACGTG [CT]AC^GT[GA]
BsaBI 4229 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 558 GACGTC G[GA]^CG[CT]C
BseAI 1547 TCCGGA T^CCGGA
BsiEI 4326 CGGCCG CG[GA][CT]^CG
BsoBI 937 CTCGAG C^[CT]CG[GA]G
BspEI 1547 TCCGGA T^CCGGA
BspLU11I 1052 ACATGT A^CATGT
BssSI 2159 CTCGTG CACGAG(-5/-1)
BstAPI 3243 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3153 CCTCAGG CC^T[ACGT]AGG
EagI 4326 CGGCCG C^GGCCG
Eam1105I 1577 GACGACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4326 CGGCCG C^GGCCG
Eco47III 3513 AGCGCT AGC^GCT
Eco52I 4326 CGGCCG C^GGCCG
Eco81I 3153 CCTCAGG CC^T[ACGT]AGG
EcoNI 1423 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 310 GAATTC G^AATTC
EcoRV 2309 GATATC GAT^ATC
FbaI 262 TGATCA T^GATCA
FspI 2466 TGCGCA TGC^GCA
Hin1I 558 GACGTC G[GA]^CG[CT]C
HincII 1940 GTTAAC GT[CT]^[GA]AC
HindII 1940 GTTAAC GT[CT]^[GA]AC
HpaI 1940 GTTAAC GTT^AAC
MamI 4229 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1547 TCCGGA T^CCGGA
NheI 1357 GCTAGC G^CTAGC
PaeR7I 937 CTCGAG C^TCGAG
PmaCI 1150 CACGTG CAC^GTG
PmlI 1150 CACGTG CAC^GTG
PpuMI 2926 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 3543 CTGCAG CTGCA^G
SacI 134 GAGCTC GAGCT^C
SexAI 3563 ACCAGGT A^CC[AT]GGT
SfiI 3687 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 3695 AGGCCT AGG^CCT
SwaI 3736 ATTTAAAT ATTT^AAAT
XbaI 629 TCTAGA T^CTAGA
XhoI 937 CTCGAG C^TCGAG
XmnI 736 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 4118 CGCG CG^CG
4667 CGCG
Alw44I 4997 GTGCAC G^TGCAC
5010 GTGCAC
AlwI 4229 GATCC GGATC(4/5)
2388 GGATC
ApaLI 4997 GTGCAC G^TGCAC
5010 GTGCAC
AspI 2039 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3175 GACTTAGTC
BciVI 1768 GGATAC GTATCC(6/5)
3775 GTATCC
BlpI 1684 GCTCAGC GC^T[ACGT]AGC
2899 GCTGAGC
Bpu1102I 1684 GCTCAGC GC^T[ACGT]AGC
2899 GCTGAGC
BsaI 3849 GAGACC GGTCTC(1/5)
2448 GGTCTC
BsaWI 1547 TCCGGA [AT]^CCGG[AT]
2730 ACCGGA
BsmBI 1964 CGTCTC CGTCTC(1/5)
4302 CGTCTC
Bsp1407I 1777 TGTACA T^GTACA
3558 TGTACA
BsrDI 903 GCAATG GCAATG(2/0)
2064 GCAATG
BsrGI 1777 TGTACA T^GTACA
3558 TGTACA
BstUI 4118 CGCG CG^CG
4667 CGCG
CelII 1684 GCTCAGC GC^T[ACGT]AGC
2899 GCTGAGC
DraIII 1871 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4528 CACTTTGTG
DrdI 2105 GACCCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4829 GACTCATCTGTC
EcoT22I 587 ATGCAT ATGCA^T
4892 ATGCAT
MvnI 4118 CGCG CG^CG
4667 CGCG
NsiI 587 ATGCAT ATGCA^T
4892 ATGCAT
PflFI 2039 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3175 GACTTAGTC
ScaI 722 AGTACT AGT^ACT
3362 AGTACT
SspBI 1777 TGTACA T^GTACA
3558 TGTACA
Tth111I 2039 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3175 GACTTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BfrI BglI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshAI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII