##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccIII AflII AflIII AscI AseI
AsnI Asp700I AviII AvrII BciVI
BfrI BlnI BlpI Bpu1102I BsaBI
BseAI BsiWI BsmBI BspEI BspLU11I
Bst1107I BstAPI BstZ17I CelII CpoI
DraIII EcoRI EcoRV EcoT22I FspI
HindIII KspI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI PacI PmeI PshAI PshBI
PvuI RsrII SacI SacII ScaI
SnaBI SphI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 972 GACGTC GACGT^C
AclI 2004 AACGTT AA^CGTT
AcsI 487 AAATTC [GA]^AATT[CT]
AgeI 642 ACCGGT A^CCGGT
Alw44I 672 GTGCAC G^TGCAC
AocI 770 CCTCAGG CC^T[ACGT]AGG
Aor51HI 76 AGCGCT AGC^GCT
ApaLI 672 GTGCAC G^TGCAC
ApoI 487 AAATTC [GA]^AATT[CT]
BamHI 2866 GGATCC G^GATCC
BbeI 236 GGCGCC GGCGC^C
BbrPI 3799 CACGTG CAC^GTG
BbvCI 2627 CCTCAGC CCTCAGC(-5/-2)
BcgI 2540 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2845 TGATCA T^GATCA
BspDI 2731 ATCGAT AT^CGAT
BssSI 3848 CACGAG CACGAG(-5/-1)
BstBI 4304 TTCGAA TT^CGAA
BstEII 1833 GGTAACC G^GT[ACGT]ACC
BstPI 1833 GGTAACC G^GT[ACGT]ACC
Bsu36I 770 CCTCAGG CC^T[ACGT]AGG
ClaI 2731 ATCGAT AT^CGAT
Eco47III 76 AGCGCT AGC^GCT
Eco81I 770 CCTCAGG CC^T[ACGT]AGG
EcoNI 1514 CCTCCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1833 GGTAACC G^GT[ACGT]ACC
FbaI 2845 TGATCA T^GATCA
FseI 226 GGCCGGCC GGCCGG^CC
HpaI 345 GTTAAC GTT^AAC
KasI 236 GGCGCC G^GCGCC
NarI 236 GGCGCC GG^CGCC
NdeI 2974 CATATG CA^TATG
NspV 4304 TTCGAA TT^CGAA
PaeR7I 366 CTCGAG C^TCGAG
PflMI 3434 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 642 ACCGGT A^CCGGT
PmaCI 3799 CACGTG CAC^GTG
PmlI 3799 CACGTG CAC^GTG
Psp1406I 2004 AACGTT AA^CGTT
SalI 3476 GTCGAC G^TCGAC
SapI 4232 GCTCTTC GCTCTTC(1/4)
SbfI 1922 CCTGCAGG CCTGCA^GG
SfiI 3703 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 236 GGCGCC GGC^GCC
SfuI 4304 TTCGAA TT^CGAA
SgrAI 641 CACCGGTG C[GA]^CCGG[CT]G
SpeI 3560 ACTAGT A^CTAGT
Sse8387I 1922 CCTGCAGG CCTGCA^GG
SspI 3624 AATATT AAT^ATT
Van91I 3434 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 366 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 13 GGTACC G^GTACC
4212 GGTACC
AccI 2436 GTAGAC GT^[AC][GT]AC
3476 GTCGAC
AhdI 4273 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4447 GACACTAGGTC
AlwNI 2221 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
3435 CAGCACCTG
Asp718I 13 GGTACC G^GTACC
4212 GGTACC
AspEI 4273 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4447 GACACTAGGTC
AspHI 140 GTGCTC G[AT]GC[AT]^C
672 GTGCAC
AspI 1302 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4398 GACACTGTC
BalI 2764 TGGCCA TGG^CCA
3607 TGGCCA
BglII 817 AGATCT A^GATCT
4181 AGATCT
BsaWI 553 TCCGGT [AT]^CCGG[AT]
642 ACCGGT
BsiHKAI 140 GTGCTC G[AT]GC[AT]^C
672 GTGCAC
BsmI 3116 GAATGC GAATGC(1/-1)
3695 GAATGC
Bsp1407I 3220 TGTACA T^GTACA
3914 TGTACA
BspHI 1658 TCATGA T^CATGA
2842 TCATGA
BsrBI 453 CCGCTC CCGCTC(-3/-3)
3466 GAGCGG
BsrGI 3220 TGTACA T^GTACA
3914 TGTACA
BssHII 97 GCGCGC G^CGCGC
99 GCGCGC
DraI 483 TTTAAA TTT^AAA
3530 TTTAAA
Eam1105I 4273 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4447 GACACTAGGTC
KpnI 13 GGTACC GGTAC^C
4212 GGTACC
MluNI 2764 TGGCCA TGG^CCA
3607 TGGCCA
MscI 2764 TGGCCA TGG^CCA
3607 TGGCCA
MspA1I 3129 CAGCTG C[AC]G^C[GT]G
4266 CAGCTG
NspI 2684 ACATGC [GA]CATG^[CT]
3566 GCATGT
PflFI 1302 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4398 GACACTGTC
PvuII 3129 CAGCTG CAG^CTG
4266 CAGCTG
RcaI 1658 TCATGA T^CATGA
2842 TCATGA
SmaI 1011 CCCGGG CCC^GGG
3102 CCCGGG
SspBI 3220 TGTACA T^GTACA
3914 TGTACA
StuI 610 AGGCCT AGG^CCT
1548 AGGCCT
Tth111I 1302 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4398 GACACTGTC
XmaCI 1011 CCCGGG C^CCGGG
3102 CCCGGG
XmaI 1011 CCCGGG C^CCGGG
3102 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII