################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AccIII AflII AflIII AscI AseI AsnI Asp700I AviII AvrII BciVI BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI BspEI BspLU11I Bst1107I BstAPI BstZ17I CelII CpoI DraIII EcoRI EcoRV EcoT22I FspI HindIII KspI MamI MfeI MluI MroI MunI NheI NotI NruI NsiI PacI PmeI PshAI PshBI PvuI RsrII SacI SacII ScaI SnaBI SphI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 972 GACGTC GACGT^C AclI 2004 AACGTT AA^CGTT AcsI 487 AAATTC [GA]^AATT[CT] AgeI 642 ACCGGT A^CCGGT Alw44I 672 GTGCAC G^TGCAC AocI 770 CCTCAGG CC^T[ACGT]AGG Aor51HI 76 AGCGCT AGC^GCT ApaLI 672 GTGCAC G^TGCAC ApoI 487 AAATTC [GA]^AATT[CT] BamHI 2866 GGATCC G^GATCC BbeI 236 GGCGCC GGCGC^C BbrPI 3799 CACGTG CAC^GTG BbvCI 2627 CCTCAGC CCTCAGC(-5/-2) BcgI 2540 GCACCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2845 TGATCA T^GATCA BspDI 2731 ATCGAT AT^CGAT BssSI 3848 CACGAG CACGAG(-5/-1) BstBI 4304 TTCGAA TT^CGAA BstEII 1833 GGTAACC G^GT[ACGT]ACC BstPI 1833 GGTAACC G^GT[ACGT]ACC Bsu36I 770 CCTCAGG CC^T[ACGT]AGG ClaI 2731 ATCGAT AT^CGAT Eco47III 76 AGCGCT AGC^GCT Eco81I 770 CCTCAGG CC^T[ACGT]AGG EcoNI 1514 CCTCCGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1833 GGTAACC G^GT[ACGT]ACC FbaI 2845 TGATCA T^GATCA FseI 226 GGCCGGCC GGCCGG^CC HpaI 345 GTTAAC GTT^AAC KasI 236 GGCGCC G^GCGCC NarI 236 GGCGCC GG^CGCC NdeI 2974 CATATG CA^TATG NspV 4304 TTCGAA TT^CGAA PaeR7I 366 CTCGAG C^TCGAG PflMI 3434 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 642 ACCGGT A^CCGGT PmaCI 3799 CACGTG CAC^GTG PmlI 3799 CACGTG CAC^GTG Psp1406I 2004 AACGTT AA^CGTT SalI 3476 GTCGAC G^TCGAC SapI 4232 GCTCTTC GCTCTTC(1/4) SbfI 1922 CCTGCAGG CCTGCA^GG SfiI 3703 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 236 GGCGCC GGC^GCC SfuI 4304 TTCGAA TT^CGAA SgrAI 641 CACCGGTG C[GA]^CCGG[CT]G SpeI 3560 ACTAGT A^CTAGT Sse8387I 1922 CCTGCAGG CCTGCA^GG SspI 3624 AATATT AAT^ATT Van91I 3434 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 366 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 13 GGTACC G^GTACC 4212 GGTACC AccI 2436 GTAGAC GT^[AC][GT]AC 3476 GTCGAC AhdI 4273 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4447 GACACTAGGTC AlwNI 2221 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 3435 CAGCACCTG Asp718I 13 GGTACC G^GTACC 4212 GGTACC AspEI 4273 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4447 GACACTAGGTC AspHI 140 GTGCTC G[AT]GC[AT]^C 672 GTGCAC AspI 1302 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4398 GACACTGTC BalI 2764 TGGCCA TGG^CCA 3607 TGGCCA BglII 817 AGATCT A^GATCT 4181 AGATCT BsaWI 553 TCCGGT [AT]^CCGG[AT] 642 ACCGGT BsiHKAI 140 GTGCTC G[AT]GC[AT]^C 672 GTGCAC BsmI 3116 GAATGC GAATGC(1/-1) 3695 GAATGC Bsp1407I 3220 TGTACA T^GTACA 3914 TGTACA BspHI 1658 TCATGA T^CATGA 2842 TCATGA BsrBI 453 CCGCTC CCGCTC(-3/-3) 3466 GAGCGG BsrGI 3220 TGTACA T^GTACA 3914 TGTACA BssHII 97 GCGCGC G^CGCGC 99 GCGCGC DraI 483 TTTAAA TTT^AAA 3530 TTTAAA Eam1105I 4273 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4447 GACACTAGGTC KpnI 13 GGTACC GGTAC^C 4212 GGTACC MluNI 2764 TGGCCA TGG^CCA 3607 TGGCCA MscI 2764 TGGCCA TGG^CCA 3607 TGGCCA MspA1I 3129 CAGCTG C[AC]G^C[GT]G 4266 CAGCTG NspI 2684 ACATGC [GA]CATG^[CT] 3566 GCATGT PflFI 1302 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4398 GACACTGTC PvuII 3129 CAGCTG CAG^CTG 4266 CAGCTG RcaI 1658 TCATGA T^CATGA 2842 TCATGA SmaI 1011 CCCGGG CCC^GGG 3102 CCCGGG SspBI 3220 TGTACA T^GTACA 3914 TGTACA StuI 610 AGGCCT AGG^CCT 1548 AGGCCT Tth111I 1302 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4398 GACACTGTC XmaCI 1011 CCCGGG C^CCGGG 3102 CCCGGG XmaI 1011 CCCGGG C^CCGGG 3102 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII