##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AccIII      AflII       AflIII      AscI        AseI        
AsnI        Asp700I     AviII       AvrII       BciVI       
BfrI        BlnI        BlpI        Bpu1102I    BsaBI       
BseAI       BsiWI       BsmBI       BspEI       BspLU11I    
Bst1107I    BstAPI      BstZ17I     CelII       CpoI        
DraIII      EcoRI       EcoRV       EcoT22I     FspI        
HindIII     KspI        MamI        MfeI        MluI        
MroI        MunI        NheI        NotI        NruI        
NsiI        PacI        PmeI        PshAI       PshBI       
PvuI        RsrII       SacI        SacII       ScaI        
SnaBI       SphI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         972  GACGTC               GACGT^C
AclI         2004  AACGTT               AA^CGTT
AcsI          487  AAATTC               [GA]^AATT[CT]
AgeI          642  ACCGGT               A^CCGGT
Alw44I        672  GTGCAC               G^TGCAC
AocI          770  CCTCAGG              CC^T[ACGT]AGG
Aor51HI        76  AGCGCT               AGC^GCT
ApaLI         672  GTGCAC               G^TGCAC
ApoI          487  AAATTC               [GA]^AATT[CT]
BamHI        2866  GGATCC               G^GATCC
BbeI          236  GGCGCC               GGCGC^C
BbrPI        3799  CACGTG               CAC^GTG
BbvCI        2627  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2540  GCACCTCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2845  TGATCA               T^GATCA
BspDI        2731  ATCGAT               AT^CGAT
BssSI        3848  CACGAG               CACGAG(-5/-1)
BstBI        4304  TTCGAA               TT^CGAA
BstEII       1833  GGTAACC              G^GT[ACGT]ACC
BstPI        1833  GGTAACC              G^GT[ACGT]ACC
Bsu36I        770  CCTCAGG              CC^T[ACGT]AGG
ClaI         2731  ATCGAT               AT^CGAT
Eco47III       76  AGCGCT               AGC^GCT
Eco81I        770  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1514  CCTCCGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1833  GGTAACC              G^GT[ACGT]ACC
FbaI         2845  TGATCA               T^GATCA
FseI          226  GGCCGGCC             GGCCGG^CC
HpaI          345  GTTAAC               GTT^AAC
KasI          236  GGCGCC               G^GCGCC
NarI          236  GGCGCC               GG^CGCC
NdeI         2974  CATATG               CA^TATG
NspV         4304  TTCGAA               TT^CGAA
PaeR7I        366  CTCGAG               C^TCGAG
PflMI        3434  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         642  ACCGGT               A^CCGGT
PmaCI        3799  CACGTG               CAC^GTG
PmlI         3799  CACGTG               CAC^GTG
Psp1406I     2004  AACGTT               AA^CGTT
SalI         3476  GTCGAC               G^TCGAC
SapI         4232  GCTCTTC              GCTCTTC(1/4)
SbfI         1922  CCTGCAGG             CCTGCA^GG
SfiI         3703  GGCCAGGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          236  GGCGCC               GGC^GCC
SfuI         4304  TTCGAA               TT^CGAA
SgrAI         641  CACCGGTG             C[GA]^CCGG[CT]G
SpeI         3560  ACTAGT               A^CTAGT
Sse8387I     1922  CCTGCAGG             CCTGCA^GG
SspI         3624  AATATT               AAT^ATT
Van91I       3434  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          366  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

Acc65I         13  GGTACC               G^GTACC
             4212  GGTACC              
AccI         2436  GTAGAC               GT^[AC][GT]AC
             3476  GTCGAC              
AhdI         4273  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4447  GACACTAGGTC         
AlwNI        2221  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3435  CAGCACCTG           
Asp718I        13  GGTACC               G^GTACC
             4212  GGTACC              
AspEI        4273  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4447  GACACTAGGTC         
AspHI         140  GTGCTC               G[AT]GC[AT]^C
              672  GTGCAC              
AspI         1302  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4398  GACACTGTC           
BalI         2764  TGGCCA               TGG^CCA
             3607  TGGCCA              
BglII         817  AGATCT               A^GATCT
             4181  AGATCT              
BsaWI         553  TCCGGT               [AT]^CCGG[AT]
              642  ACCGGT              
BsiHKAI       140  GTGCTC               G[AT]GC[AT]^C
              672  GTGCAC              
BsmI         3116  GAATGC               GAATGC(1/-1)
             3695  GAATGC              
Bsp1407I     3220  TGTACA               T^GTACA
             3914  TGTACA              
BspHI        1658  TCATGA               T^CATGA
             2842  TCATGA              
BsrBI         453  CCGCTC               CCGCTC(-3/-3)
             3466  GAGCGG              
BsrGI        3220  TGTACA               T^GTACA
             3914  TGTACA              
BssHII         97  GCGCGC               G^CGCGC
               99  GCGCGC              
DraI          483  TTTAAA               TTT^AAA
             3530  TTTAAA              
Eam1105I     4273  GACATCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4447  GACACTAGGTC         
KpnI           13  GGTACC               GGTAC^C
             4212  GGTACC              
MluNI        2764  TGGCCA               TGG^CCA
             3607  TGGCCA              
MscI         2764  TGGCCA               TGG^CCA
             3607  TGGCCA              
MspA1I       3129  CAGCTG               C[AC]G^C[GT]G
             4266  CAGCTG              
NspI         2684  ACATGC               [GA]CATG^[CT]
             3566  GCATGT              
PflFI        1302  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4398  GACACTGTC           
PvuII        3129  CAGCTG               CAG^CTG
             4266  CAGCTG              
RcaI         1658  TCATGA               T^CATGA
             2842  TCATGA              
SmaI         1011  CCCGGG               CCC^GGG
             3102  CCCGGG              
SspBI        3220  TGTACA               T^GTACA
             3914  TGTACA              
StuI          610  AGGCCT               AGG^CCT
             1548  AGGCCT              
Tth111I      1302  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4398  GACACTGTC           
XmaCI        1011  CCCGGG               C^CCGGG
             3102  CCCGGG              
XmaI         1011  CCCGGG               C^CCGGG
             3102  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AvaI        AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DrdI        
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII