##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AvrII BbeI BbrPI
BclI BfrI BlnI BsiWI BspDI
Bst1107I BstBI BstZ17I ClaI DraI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KasI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I SalI SapI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 960 GACGTC GACGT^C
Acc65I 4043 GGTACC G^GTACC
Asp718I 4043 GGTACC G^GTACC
AspI 2684 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3837 TGCGCA TGC^GCA
BbsI 3148 GTCTTC GAAGAC(2/6)
BbvCI 1346 GCTGAGG CCTCAGC(-5/-2)
BcgI 644 CGATTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2928 GTATCC GTATCC(6/5)
BglII 2289 AGATCT A^GATCT
BpuAI 3148 GTCTTC GAAGAC(2/6)
BsmI 3831 GCATTC GAATGC(1/-1)
Bsp1407I 2901 TGTACA T^GTACA
BspHI 3939 TCATGA T^CATGA
BsrGI 2901 TGTACA T^GTACA
CpoI 3215 CGGTCCG CG^G[AT]CCG
DraIII 1796 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3721 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3837 TGCGCA TGC^GCA
HindIII 2600 AAGCTT A^AGCTT
KpnI 4043 GGTACC GGTAC^C
PaeR7I 1809 CTCGAG C^TCGAG
PflFI 2684 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2861 CGATCG CGAT^CG
RcaI 3939 TCATGA T^CATGA
RsrII 3215 CGGTCCG CG^G[AT]CCG
SbfI 1581 CCTGCAGG CCTGCA^GG
ScaI 1436 AGTACT AGT^ACT
SfiI 2608 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 4798 GCATGC GCATG^C
Sse8387I 1581 CCTGCAGG CCTGCA^GG
SspBI 2901 TGTACA T^GTACA
Tth111I 2684 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1809 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 590 GTCTAC GT^[AC][GT]AC
3875 GTAGAC
AccIII 3027 TCCGGA T^CCGGA
3408 TCCGGA
AcyI 960 GACGTC G[GA]^CG[CT]C
4903 GACGCC
BsaAI 3182 TACGTG [CT]AC^GT[GA]
4319 TACGTG
BsaBI 3601 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3959 GATGTGGATC
BsaHI 960 GACGTC G[GA]^CG[CT]C
4903 GACGCC
BseAI 3027 TCCGGA T^CCGGA
3408 TCCGGA
BsmBI 2179 CGTCTC CGTCTC(1/5)
4944 CGTCTC
BspEI 3027 TCCGGA T^CCGGA
3408 TCCGGA
BssHII 345 GCGCGC G^CGCGC
347 GCGCGC
BssSI 4312 CACGAG CACGAG(-5/-1)
3626 CTCGTG
BstAPI 1195 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4433 GCACCTGATGC
EagI 551 CGGCCG C^GGCCG
3400 CGGCCG
EclXI 551 CGGCCG C^GGCCG
3400 CGGCCG
Eco52I 551 CGGCCG C^GGCCG
3400 CGGCCG
Hin1I 960 GACGTC G[GA]^CG[CT]C
4903 GACGCC
KspI 233 CCGCGG CCGC^GG
5187 CCGCGG
MamI 3601 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3959 GATGTGGATC
MroI 3027 TCCGGA T^CCGGA
3408 TCCGGA
MseI 4263 TTAA T^TAA
5612 TTAA
NcoI 1361 CCATGG C^CATGG
3096 CCATGG
PshAI 489 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5029 GACCGAGGTC
SacII 233 CCGCGG CCGC^GG
5187 CCGCGG
SexAI 781 ACCTGGT A^CC[AT]GGT
2565 ACCAGGT
SmlI 968 CTTGAG C^T[CT][GA]AG
1809 CTCGAG
Tru9I 4263 TTAA T^TAA
5612 TTAA
Tsp509I 3233 AATT ^AATT
5610 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI