################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AvrII BbeI BbrPI BclI BfrI BlnI BsiWI BspDI Bst1107I BstBI BstZ17I ClaI DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KasI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I SalI SapI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AatII 960 GACGTC GACGT^C Acc65I 4043 GGTACC G^GTACC Asp718I 4043 GGTACC G^GTACC AspI 2684 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3837 TGCGCA TGC^GCA BbsI 3148 GTCTTC GAAGAC(2/6) BbvCI 1346 GCTGAGG CCTCAGC(-5/-2) BcgI 644 CGATTCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2928 GTATCC GTATCC(6/5) BglII 2289 AGATCT A^GATCT BpuAI 3148 GTCTTC GAAGAC(2/6) BsmI 3831 GCATTC GAATGC(1/-1) Bsp1407I 2901 TGTACA T^GTACA BspHI 3939 TCATGA T^CATGA BsrGI 2901 TGTACA T^GTACA CpoI 3215 CGGTCCG CG^G[AT]CCG DraIII 1796 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3721 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 3837 TGCGCA TGC^GCA HindIII 2600 AAGCTT A^AGCTT KpnI 4043 GGTACC GGTAC^C PaeR7I 1809 CTCGAG C^TCGAG PflFI 2684 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 2861 CGATCG CGAT^CG RcaI 3939 TCATGA T^CATGA RsrII 3215 CGGTCCG CG^G[AT]CCG SbfI 1581 CCTGCAGG CCTGCA^GG ScaI 1436 AGTACT AGT^ACT SfiI 2608 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 4798 GCATGC GCATG^C Sse8387I 1581 CCTGCAGG CCTGCA^GG SspBI 2901 TGTACA T^GTACA Tth111I 2684 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1809 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 590 GTCTAC GT^[AC][GT]AC 3875 GTAGAC AccIII 3027 TCCGGA T^CCGGA 3408 TCCGGA AcyI 960 GACGTC G[GA]^CG[CT]C 4903 GACGCC BsaAI 3182 TACGTG [CT]AC^GT[GA] 4319 TACGTG BsaBI 3601 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3959 GATGTGGATC BsaHI 960 GACGTC G[GA]^CG[CT]C 4903 GACGCC BseAI 3027 TCCGGA T^CCGGA 3408 TCCGGA BsmBI 2179 CGTCTC CGTCTC(1/5) 4944 CGTCTC BspEI 3027 TCCGGA T^CCGGA 3408 TCCGGA BssHII 345 GCGCGC G^CGCGC 347 GCGCGC BssSI 4312 CACGAG CACGAG(-5/-1) 3626 CTCGTG BstAPI 1195 GCAAGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4433 GCACCTGATGC EagI 551 CGGCCG C^GGCCG 3400 CGGCCG EclXI 551 CGGCCG C^GGCCG 3400 CGGCCG Eco52I 551 CGGCCG C^GGCCG 3400 CGGCCG Hin1I 960 GACGTC G[GA]^CG[CT]C 4903 GACGCC KspI 233 CCGCGG CCGC^GG 5187 CCGCGG MamI 3601 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3959 GATGTGGATC MroI 3027 TCCGGA T^CCGGA 3408 TCCGGA MseI 4263 TTAA T^TAA 5612 TTAA NcoI 1361 CCATGG C^CATGG 3096 CCATGG PshAI 489 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5029 GACCGAGGTC SacII 233 CCGCGG CCGC^GG 5187 CCGCGG SexAI 781 ACCTGGT A^CC[AT]GGT 2565 ACCAGGT SmlI 968 CTTGAG C^T[CT][GA]AG 1809 CTCGAG Tru9I 4263 TTAA T^TAA 5612 TTAA Tsp509I 3233 AATT ^AATT 5610 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI