##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI ApaI AscI AseI
AsnI Asp718I AspEI AviII BbeI
BcgI BciVI BclI BglI BsaBI
BsaHI BsiEI BsiWI BspDI BspHI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI FbaI
FseI FspI Hin1I HindIII KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NgoMIV
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AocI 3425 CCTTAGG CC^T[ACGT]AGG
Aor51HI 517 AGCGCT AGC^GCT
AspI 4837 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5005 CTCGAG C^[CT]CG[GA]G
BamHI 152 GGATCC G^GATCC
BbrPI 1687 CACGTG CAC^GTG
BglII 2004 AGATCT A^GATCT
BsmBI 1405 CGTCTC CGTCTC(1/5)
BsoBI 5005 CTCGAG C^[CT]CG[GA]G
BsrFI 531 GCCGGT [GA]^CCGG[CT]
Bsu36I 3425 CCTTAGG CC^T[ACGT]AGG
Cfr10I 531 GCCGGT [GA]^CCGG[CT]
Eco47III 517 AGCGCT AGC^GCT
Eco81I 3425 CCTTAGG CC^T[ACGT]AGG
EcoNI 1105 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 517 AGCGCT [GA]GCGC^[CT]
HincII 1528 GTTAAC GT[CT]^[GA]AC
HindII 1528 GTTAAC GT[CT]^[GA]AC
HpaI 1528 GTTAAC GTT^AAC
NdeI 3140 CATATG CA^TATG
PaeR7I 5005 CTCGAG C^TCGAG
PflFI 4837 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1687 CACGTG CAC^GTG
PmlI 1687 CACGTG CAC^GTG
PstI 1564 CTGCAG CTGCA^G
PvuII 138 CAGCTG CAG^CTG
SnaBI 4950 TACGTA TAC^GTA
SwaI 4967 ATTTAAAT ATTT^AAAT
Tth111I 4837 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3372 TCTAGA T^CTAGA
XhoI 5005 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 3700 GTCTAC GT^[AC][GT]AC
3757 GTAGAC
AccIII 761 TCCGGA T^CCGGA
5382 TCCGGA
Alw44I 732 GTGCAC G^TGCAC
5029 GTGCAC
ApaLI 732 GTGCAC G^TGCAC
5029 GTGCAC
Asp700I 596 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3280 GAATGATTTC
BalI 2838 TGGCCA TGG^CCA
4343 TGGCCA
BanI 1762 GGTGCC G^G[CT][GA]CC
4932 GGCACC
BbvCI 3062 CCTCAGC CCTCAGC(-5/-2)
5375 CCTCAGC
BlpI 2169 GCTGAGC GC^T[ACGT]AGC
3654 GCTGAGC
Bpu1102I 2169 GCTGAGC GC^T[ACGT]AGC
3654 GCTGAGC
BsaI 1645 GAGACC GGTCTC(1/5)
16 GGTCTC
BsaWI 761 TCCGGA [AT]^CCGG[AT]
5382 TCCGGA
BseAI 761 TCCGGA T^CCGGA
5382 TCCGGA
Bsp1407I 1452 TGTACA T^GTACA
1854 TGTACA
BspEI 761 TCCGGA T^CCGGA
5382 TCCGGA
BspLU11I 2880 ACATGT A^CATGT
2926 ACATGT
BsrGI 1452 TGTACA T^GTACA
1854 TGTACA
BstAPI 892 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2150 GCAGTCAGTGC
BstXI 2829 CCAGCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5356 CCAAGGCCCTGG
CelII 2169 GCTGAGC GC^T[ACGT]AGC
3654 GCTGAGC
CfoI 518 GCGC GCG^C
1426 GCGC
DraIII 1787 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2107 CACCATGTG
EcoRV 3038 GATATC GAT^ATC
4197 GATATC
EcoT22I 2202 ATGCAT ATGCA^T
3672 ATGCAT
HgaI 32 GCGTC GACGC(5/10)
1404 GCGTC
HhaI 518 GCGC GCG^C
1426 GCGC
HinP1I 518 GCGC G^CGC
1426 GCGC
MluNI 2838 TGGCCA TGG^CCA
4343 TGGCCA
MroI 761 TCCGGA T^CCGGA
5382 TCCGGA
MscI 2838 TGGCCA TGG^CCA
4343 TGGCCA
MspA1I 138 CAGCTG C[AC]G^C[GT]G
2830 CAGCGG
NsiI 2202 ATGCAT ATGCA^T
3672 ATGCAT
PflMI 2106 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2665 CCATCTTTTGG
PshAI 1659 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3354 GACTTGTGTC
ScaI 3085 AGTACT AGT^ACT
3448 AGTACT
SspBI 1452 TGTACA T^GTACA
1854 TGTACA
SspI 2912 AATATT AAT^ATT
4477 AATATT
Van91I 2106 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2665 CCATCTTTTGG
XcmI 2109 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2310 CCACGCAGGTGCTGG
XmnI 596 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3280 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII AvrII BanII BbsI BbvI
BcnI BfaI BfrI BlnI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NheI NlaIII NlaIV NspI
PleI PpuMI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII