################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AviII BbeI BcgI BciVI BclI BglI BsaBI BsaHI BsiEI BsiWI BspDI BspHI BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI FbaI FseI FspI Hin1I HindIII KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AocI 3425 CCTTAGG CC^T[ACGT]AGG Aor51HI 517 AGCGCT AGC^GCT AspI 4837 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 5005 CTCGAG C^[CT]CG[GA]G BamHI 152 GGATCC G^GATCC BbrPI 1687 CACGTG CAC^GTG BglII 2004 AGATCT A^GATCT BsmBI 1405 CGTCTC CGTCTC(1/5) BsoBI 5005 CTCGAG C^[CT]CG[GA]G BsrFI 531 GCCGGT [GA]^CCGG[CT] Bsu36I 3425 CCTTAGG CC^T[ACGT]AGG Cfr10I 531 GCCGGT [GA]^CCGG[CT] Eco47III 517 AGCGCT AGC^GCT Eco81I 3425 CCTTAGG CC^T[ACGT]AGG EcoNI 1105 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 517 AGCGCT [GA]GCGC^[CT] HincII 1528 GTTAAC GT[CT]^[GA]AC HindII 1528 GTTAAC GT[CT]^[GA]AC HpaI 1528 GTTAAC GTT^AAC NdeI 3140 CATATG CA^TATG PaeR7I 5005 CTCGAG C^TCGAG PflFI 4837 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1687 CACGTG CAC^GTG PmlI 1687 CACGTG CAC^GTG PstI 1564 CTGCAG CTGCA^G PvuII 138 CAGCTG CAG^CTG SnaBI 4950 TACGTA TAC^GTA SwaI 4967 ATTTAAAT ATTT^AAAT Tth111I 4837 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3372 TCTAGA T^CTAGA XhoI 5005 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 3700 GTCTAC GT^[AC][GT]AC 3757 GTAGAC AccIII 761 TCCGGA T^CCGGA 5382 TCCGGA Alw44I 732 GTGCAC G^TGCAC 5029 GTGCAC ApaLI 732 GTGCAC G^TGCAC 5029 GTGCAC Asp700I 596 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3280 GAATGATTTC BalI 2838 TGGCCA TGG^CCA 4343 TGGCCA BanI 1762 GGTGCC G^G[CT][GA]CC 4932 GGCACC BbvCI 3062 CCTCAGC CCTCAGC(-5/-2) 5375 CCTCAGC BlpI 2169 GCTGAGC GC^T[ACGT]AGC 3654 GCTGAGC Bpu1102I 2169 GCTGAGC GC^T[ACGT]AGC 3654 GCTGAGC BsaI 1645 GAGACC GGTCTC(1/5) 16 GGTCTC BsaWI 761 TCCGGA [AT]^CCGG[AT] 5382 TCCGGA BseAI 761 TCCGGA T^CCGGA 5382 TCCGGA Bsp1407I 1452 TGTACA T^GTACA 1854 TGTACA BspEI 761 TCCGGA T^CCGGA 5382 TCCGGA BspLU11I 2880 ACATGT A^CATGT 2926 ACATGT BsrGI 1452 TGTACA T^GTACA 1854 TGTACA BstAPI 892 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2150 GCAGTCAGTGC BstXI 2829 CCAGCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5356 CCAAGGCCCTGG CelII 2169 GCTGAGC GC^T[ACGT]AGC 3654 GCTGAGC CfoI 518 GCGC GCG^C 1426 GCGC DraIII 1787 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2107 CACCATGTG EcoRV 3038 GATATC GAT^ATC 4197 GATATC EcoT22I 2202 ATGCAT ATGCA^T 3672 ATGCAT HgaI 32 GCGTC GACGC(5/10) 1404 GCGTC HhaI 518 GCGC GCG^C 1426 GCGC HinP1I 518 GCGC G^CGC 1426 GCGC MluNI 2838 TGGCCA TGG^CCA 4343 TGGCCA MroI 761 TCCGGA T^CCGGA 5382 TCCGGA MscI 2838 TGGCCA TGG^CCA 4343 TGGCCA MspA1I 138 CAGCTG C[AC]G^C[GT]G 2830 CAGCGG NsiI 2202 ATGCAT ATGCA^T 3672 ATGCAT PflMI 2106 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2665 CCATCTTTTGG PshAI 1659 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3354 GACTTGTGTC ScaI 3085 AGTACT AGT^ACT 3448 AGTACT SspBI 1452 TGTACA T^GTACA 1854 TGTACA SspI 2912 AATATT AAT^ATT 4477 AATATT Van91I 2106 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2665 CCATCTTTTGG XcmI 2109 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2310 CCACGCAGGTGCTGG XmnI 596 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3280 GAATGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI AspHI AvaII AvrII BanII BbsI BbvI BcnI BfaI BfrI BlnI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PleI PpuMI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII