################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AatII AccII AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AvrII BbrPI BlnI BsaAI BsiWI BsmBI BspDI BsrBI BssHII BssSI Bst1107I BstUI BstZ17I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoRI EcoRV FseI HpaI KspI MfeI MluI MunI MvnI NaeI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I RsrII SacII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 283 GGTACC G^GTACC AflII 4012 CTTAAG C^TTAAG AflIII 2459 ACATGT A^C[GA][CT]GT AhdI 3997 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 283 GGTACC G^GTACC AspEI 3997 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3509 TGCGCA TGC^GCA BbeI 4502 GGCGCC GGCGC^C BbvCI 2745 CCTCAGC CCTCAGC(-5/-2) BciVI 1427 GTATCC GTATCC(6/5) BfrI 4012 CTTAAG C^TTAAG BglI 2564 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 3924 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3924 GCTCAGC GC^T[ACGT]AGC BspLU11I 2459 ACATGT A^CATGT BsrFI 82 ACCGGC [GA]^CCGG[CT] BstBI 2363 TTCGAA TT^CGAA CelII 3924 GCTCAGC GC^T[ACGT]AGC Cfr10I 82 ACCGGC [GA]^CCGG[CT] DraI 433 TTTAAA TTT^AAA Eam1105I 3997 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 3509 TGCGCA TGC^GCA HindIII 4627 AAGCTT A^AGCTT KasI 4502 GGCGCC G^GCGCC KpnI 283 GGTACC GGTAC^C NarI 4502 GGCGCC GG^CGCC NheI 2560 GCTAGC G^CTAGC NspV 2363 TTCGAA TT^CGAA PflMI 954 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 687 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 4502 GGCGCC GGC^GCC SfuI 2363 TTCGAA TT^CGAA SmaI 4229 CCCGGG CCC^GGG Van91I 954 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3306 TCTAGA T^CTAGA XmaCI 4229 CCCGGG C^CCGGG XmaI 4229 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 394 GTAGAC GT^[AC][GT]AC 3802 GTCTAC AccIII 332 TCCGGA T^CCGGA 3470 TCCGGA AcsI 2395 GAATTT [GA]^AATT[CT] 2785 AAATTC AcyI 4495 GACGCC G[GA]^CG[CT]C 4502 GGCGCC ApoI 2395 GAATTT [GA]^AATT[CT] 2785 AAATTC Asp700I 46 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4182 GAACCGCTTC AspI 964 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3856 GACATGGTC BsaBI 237 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1326 GATGTACATC BsaHI 4495 GACGCC G[GA]^CG[CT]C 4502 GGCGCC BsaWI 332 TCCGGA [AT]^CCGG[AT] 3470 TCCGGA BseAI 332 TCCGGA T^CCGGA 3470 TCCGGA BspEI 332 TCCGGA T^CCGGA 3470 TCCGGA BspHI 141 TCATGA T^CATGA 3239 TCATGA BsrDI 3641 CATTGC GCAATG(2/0) 3174 GCAATG BstEII 422 GGTTACC G^GT[ACGT]ACC 5068 GGTGACC BstPI 422 GGTTACC G^GT[ACGT]ACC 5068 GGTGACC DrdI 542 GACCTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3793 GACTCAGAAGTC EcoNI 844 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2904 CCTTCGGGAGG EcoO65I 422 GGTTACC G^GT[ACGT]ACC 5068 GGTGACC EcoT22I 4336 ATGCAT ATGCA^T 4795 ATGCAT HgaI 4495 GACGC GACGC(5/10) 4085 GCGTC Hin1I 4495 GACGCC G[GA]^CG[CT]C 4502 GGCGCC MaeII 549 ACGT A^CGT 968 ACGT MamI 237 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1326 GATGTACATC MroI 332 TCCGGA T^CCGGA 3470 TCCGGA NcoI 3813 CCATGG C^CATGG 5186 CCATGG NsiI 4336 ATGCAT ATGCA^T 4795 ATGCAT PflFI 964 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3856 GACATGGTC PvuI 2639 CGATCG CGAT^CG 3838 CGATCG PvuII 406 CAGCTG CAG^CTG 3217 CAGCTG RcaI 141 TCATGA T^CATGA 3239 TCATGA SexAI 570 ACCAGGT A^CC[AT]GGT 3557 ACCAGGT Tth111I 964 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC 3856 GACATGGTC XmnI 46 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4182 GAACCGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcgI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII