##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccII AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AvrII BbrPI BlnI BsaAI BsiWI
BsmBI BspDI BsrBI BssHII BssSI
Bst1107I BstUI BstZ17I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoRI EcoRV FseI HpaI KspI
MfeI MluI MunI MvnI NaeI
NgoMIV NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RsrII SacII SalI SbfI
ScaI SfiI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 283 GGTACC G^GTACC
AflII 4012 CTTAAG C^TTAAG
AflIII 2459 ACATGT A^C[GA][CT]GT
AhdI 3997 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 283 GGTACC G^GTACC
AspEI 3997 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3509 TGCGCA TGC^GCA
BbeI 4502 GGCGCC GGCGC^C
BbvCI 2745 CCTCAGC CCTCAGC(-5/-2)
BciVI 1427 GTATCC GTATCC(6/5)
BfrI 4012 CTTAAG C^TTAAG
BglI 2564 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3924 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3924 GCTCAGC GC^T[ACGT]AGC
BspLU11I 2459 ACATGT A^CATGT
BsrFI 82 ACCGGC [GA]^CCGG[CT]
BstBI 2363 TTCGAA TT^CGAA
CelII 3924 GCTCAGC GC^T[ACGT]AGC
Cfr10I 82 ACCGGC [GA]^CCGG[CT]
DraI 433 TTTAAA TTT^AAA
Eam1105I 3997 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3509 TGCGCA TGC^GCA
HindIII 4627 AAGCTT A^AGCTT
KasI 4502 GGCGCC G^GCGCC
KpnI 283 GGTACC GGTAC^C
NarI 4502 GGCGCC GG^CGCC
NheI 2560 GCTAGC G^CTAGC
NspV 2363 TTCGAA TT^CGAA
PflMI 954 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 687 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 4502 GGCGCC GGC^GCC
SfuI 2363 TTCGAA TT^CGAA
SmaI 4229 CCCGGG CCC^GGG
Van91I 954 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3306 TCTAGA T^CTAGA
XmaCI 4229 CCCGGG C^CCGGG
XmaI 4229 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 394 GTAGAC GT^[AC][GT]AC
3802 GTCTAC
AccIII 332 TCCGGA T^CCGGA
3470 TCCGGA
AcsI 2395 GAATTT [GA]^AATT[CT]
2785 AAATTC
AcyI 4495 GACGCC G[GA]^CG[CT]C
4502 GGCGCC
ApoI 2395 GAATTT [GA]^AATT[CT]
2785 AAATTC
Asp700I 46 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4182 GAACCGCTTC
AspI 964 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3856 GACATGGTC
BsaBI 237 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATGTACATC
BsaHI 4495 GACGCC G[GA]^CG[CT]C
4502 GGCGCC
BsaWI 332 TCCGGA [AT]^CCGG[AT]
3470 TCCGGA
BseAI 332 TCCGGA T^CCGGA
3470 TCCGGA
BspEI 332 TCCGGA T^CCGGA
3470 TCCGGA
BspHI 141 TCATGA T^CATGA
3239 TCATGA
BsrDI 3641 CATTGC GCAATG(2/0)
3174 GCAATG
BstEII 422 GGTTACC G^GT[ACGT]ACC
5068 GGTGACC
BstPI 422 GGTTACC G^GT[ACGT]ACC
5068 GGTGACC
DrdI 542 GACCTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3793 GACTCAGAAGTC
EcoNI 844 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2904 CCTTCGGGAGG
EcoO65I 422 GGTTACC G^GT[ACGT]ACC
5068 GGTGACC
EcoT22I 4336 ATGCAT ATGCA^T
4795 ATGCAT
HgaI 4495 GACGC GACGC(5/10)
4085 GCGTC
Hin1I 4495 GACGCC G[GA]^CG[CT]C
4502 GGCGCC
MaeII 549 ACGT A^CGT
968 ACGT
MamI 237 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1326 GATGTACATC
MroI 332 TCCGGA T^CCGGA
3470 TCCGGA
NcoI 3813 CCATGG C^CATGG
5186 CCATGG
NsiI 4336 ATGCAT ATGCA^T
4795 ATGCAT
PflFI 964 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3856 GACATGGTC
PvuI 2639 CGATCG CGAT^CG
3838 CGATCG
PvuII 406 CAGCTG CAG^CTG
3217 CAGCTG
RcaI 141 TCATGA T^CATGA
3239 TCATGA
SexAI 570 ACCAGGT A^CC[AT]GGT
3557 ACCAGGT
Tth111I 964 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3856 GACATGGTC
XmnI 46 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4182 GAACCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcgI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII