##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI AspI AviII BbeI BfrI
BglI BsiWI Bsp1407I BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI DraI
DrdI Eco47III EcoRI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MfeI MluI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2832 GGTACC G^GTACC
AccIII 5419 TCCGGA T^CCGGA
AcyI 2808 GACGCC G[GA]^CG[CT]C
Asp718I 2832 GGTACC G^GTACC
BbrPI 1926 CACGTG CAC^GTG
BcgI 3451 GCAGATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 914 TGATCA T^GATCA
BsaAI 1926 CACGTG [CT]AC^GT[GA]
BsaHI 2808 GACGCC G[GA]^CG[CT]C
BseAI 5419 TCCGGA T^CCGGA
BsiEI 232 CGGCCG CG[GA][CT]^CG
BsmBI 5454 GAGACG CGTCTC(1/5)
BspDI 4779 ATCGAT AT^CGAT
BspEI 5419 TCCGGA T^CCGGA
BspHI 3220 TCATGA T^CATGA
BspLU11I 3786 ACATGT A^CATGT
BspMI 3726 GCAGGT ACCTGC(4/8)
BsrBI 1758 GAGCGG CCGCTC(-3/-3)
BsrDI 5377 GCAATG GCAATG(2/0)
BsrFI 234 GCCGGC [GA]^CCGG[CT]
BssSI 1752 CTCGTG CACGAG(-5/-1)
BstEII 200 GGTGACC G^GT[ACGT]ACC
BstPI 200 GGTGACC G^GT[ACGT]ACC
Cfr10I 234 GCCGGC [GA]^CCGG[CT]
ClaI 4779 ATCGAT AT^CGAT
DraIII 3385 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 232 CGGCCG C^GGCCG
EclXI 232 CGGCCG C^GGCCG
Eco52I 232 CGGCCG C^GGCCG
EcoO65I 200 GGTGACC G^GT[ACGT]ACC
EcoRV 3212 GATATC GAT^ATC
EcoT22I 3529 ATGCAT ATGCA^T
FbaI 914 TGATCA T^GATCA
Hin1I 2808 GACGCC G[GA]^CG[CT]C
KpnI 2832 GGTACC GGTAC^C
MroI 5419 TCCGGA T^CCGGA
NaeI 234 GCCGGC GCC^GGC
NgoMIV 234 GCCGGC G^CCGGC
NsiI 3529 ATGCAT ATGCA^T
PmaCI 1926 CACGTG CAC^GTG
PmlI 1926 CACGTG CAC^GTG
RcaI 3220 TCATGA T^CATGA
SapI 289 GCTCTTC GCTCTTC(1/4)
SexAI 5462 ACCAGGT A^CC[AT]GGT
SmaI 2090 CCCGGG CCC^GGG
SphI 2031 GCATGC GCATG^C
SspI 942 AATATT AAT^ATT
XmaCI 2090 CCCGGG C^CCGGG
XmaI 2090 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1415 GTAGAC GT^[AC][GT]AC
2466 GTAGAC
AccII 476 CGCG CG^CG
5494 CGCG
AflIII 1925 ACACGT A^C[GA][CT]GT
3786 ACATGT
AhdI 25 GACGGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2480 GACCAAGAGTC
Asp700I 1142 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3415 GAAGCACTTC
AspEI 25 GACGGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2480 GACCAAGAGTC
BalI 979 TGGCCA TGG^CCA
3357 TGGCCA
BciVI 570 GTATCC GTATCC(6/5)
1687 GTATCC
BglII 3399 AGATCT A^GATCT
5800 AGATCT
BsaBI 3212 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5318 GATGTTAATC
BsaWI 3583 TCCGGT [AT]^CCGG[AT]
5419 TCCGGA
BsmI 5710 GAATGC GAATGC(1/-1)
1107 GCATTC
BstUI 476 CGCG CG^CG
5494 CGCG
Eam1105I 25 GACGGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2480 GACCAAGAGTC
HaeII 440 AGCGCC [GA]GCGC^[CT]
2108 AGCGCC
MamI 3212 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5318 GATGTTAATC
MluNI 979 TGGCCA TGG^CCA
3357 TGGCCA
MscI 979 TGGCCA TGG^CCA
3357 TGGCCA
MvnI 476 CGCG CG^CG
5494 CGCG
NcoI 2491 CCATGG C^CATGG
3617 CCATGG
SacI 1446 GAGCTC GAGCT^C
3473 GAGCTC
XbaI 143 TCTAGA T^CTAGA
4022 TCTAGA
XmnI 1142 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3415 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AseI AsnI AspHI AvaI
AvaII AvrII BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII