##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccI AclI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BciVI BclI BglII BlnI BsiEI
BsiWI BspLU11I BssSI Bst1107I BstBI
BstXI BstZ17I CpoI DraI EagI
Eam1105I EclXI Eco52I EcoRV FbaI
FseI HpaI KasI KpnI KspI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfoI SfuI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 4293 GACGTC GACGT^C
AccIII 4317 TCCGGA T^CCGGA
AcsI 4331 GAATTC [GA]^AATT[CT]
AflII 3331 CTTAAG C^TTAAG
AgeI 847 ACCGGT A^CCGGT
Alw44I 423 GTGCAC G^TGCAC
Aor51HI 41 AGCGCT AGC^GCT
ApaLI 423 GTGCAC G^TGCAC
ApoI 4331 GAATTC [GA]^AATT[CT]
BfrI 3331 CTTAAG C^TTAAG
BglI 2371 GCCGATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 217 TACGTG [CT]AC^GT[GA]
BsaBI 2944 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4317 TCCGGA T^CCGGA
BsmI 3672 GAATGC GAATGC(1/-1)
Bsp1407I 2229 TGTACA T^GTACA
BspDI 259 ATCGAT AT^CGAT
BspEI 4317 TCCGGA T^CCGGA
BspHI 554 TCATGA T^CATGA
BsrDI 1359 CATTGC GCAATG(2/0)
BsrGI 2229 TGTACA T^GTACA
BssHII 63 GCGCGC G^CGCGC
ClaI 259 ATCGAT AT^CGAT
DrdI 136 GACCGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 41 AGCGCT AGC^GCT
EcoRI 4331 GAATTC G^AATTC
EcoT22I 898 ATGCAT ATGCA^T
MamI 2944 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2953 CAATTG C^AATTG
MluI 427 ACGCGT A^CGCGT
MroI 4317 TCCGGA T^CCGGA
MunI 2953 CAATTG C^AATTG
NsiI 898 ATGCAT ATGCA^T
PaeR7I 4072 CTCGAG C^TCGAG
PinAI 847 ACCGGT A^CCGGT
RcaI 554 TCATGA T^CATGA
SapI 210 GCTCTTC GCTCTTC(1/4)
ScaI 4054 AGTACT AGT^ACT
SfiI 2370 GGCCGATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 846 CACCGGTG C[GA]^CCGG[CT]G
SspBI 2229 TGTACA T^GTACA
XhoI 4072 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 86 GACGCC G[GA]^CG[CT]C
4293 GACGTC
AflIII 427 ACGCGT A^C[GA][CT]GT
647 ACACGT
AviII 221 TGCGCA TGC^GCA
788 TGCGCA
BcgI 2633 CGACCAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4450 CGAGGCTGGTGC
BsaHI 86 GACGCC G[GA]^CG[CT]C
4293 GACGTC
BsmBI 2625 CGTCTC CGTCTC(1/5)
4291 GAGACG
BsrBI 1626 CCGCTC CCGCTC(-3/-3)
904 GAGCGG
BsrFI 847 ACCGGT [GA]^CCGG[CT]
3305 ACCGGC
BstEII 408 GGTGACC G^GT[ACGT]ACC
3264 GGTGACC
BstPI 408 GGTGACC G^GT[ACGT]ACC
3264 GGTGACC
Cfr10I 847 ACCGGT [GA]^CCGG[CT]
3305 ACCGGC
DraIII 1602 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3837 CACCCTGTG
EcoO65I 408 GGTGACC G^GT[ACGT]ACC
3264 GGTGACC
FspI 221 TGCGCA TGC^GCA
788 TGCGCA
HaeII 41 AGCGCT [GA]GCGC^[CT]
1765 AGCGCC
Hin1I 86 GACGCC G[GA]^CG[CT]C
4293 GACGTC
PshAI 2706 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3549 GACCTATGTC
SphI 2639 GCATGC GCATG^C
4489 GCATGC
StuI 3201 AGGCCT AGG^CCT
4629 AGGCCT
Tsp509I 2954 AATT ^AATT
4332 AATT
XcmI 1496 CCAGCCTCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4181 CCAGCTCCAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmaCI XmaI