##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AflII AscI AseI AsnI
AspI AviII BbvCI BclI BfrI
BglI BsiEI BsiWI BsmI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV FbaI FseI FspI
HpaI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PmeI PshAI PshBI
PvuI RsrII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3897 GGTACC G^GTACC
AccIII 1286 TCCGGA T^CCGGA
AclI 1844 AACGTT AA^CGTT
AflIII 4869 ACGTGT A^C[GA][CT]GT
AgeI 232 ACCGGT A^CCGGT
AhdI 715 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3113 GTGCAC G^TGCAC
ApaLI 3113 GTGCAC G^TGCAC
Asp700I 1872 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3897 GGTACC G^GTACC
AspEI 715 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3862 CACGTG CAC^GTG
BbsI 4710 GTCTTC GAAGAC(2/6)
BcgI 59 GCATGGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 4710 GTCTTC GAAGAC(2/6)
BsaAI 3862 CACGTG [CT]AC^GT[GA]
BseAI 1286 TCCGGA T^CCGGA
BsmBI 188 GAGACG CGTCTC(1/5)
BspEI 1286 TCCGGA T^CCGGA
BsrDI 4740 GCAATG GCAATG(2/0)
BstEII 3119 GGTGACC G^GT[ACGT]ACC
BstPI 3119 GGTGACC G^GT[ACGT]ACC
DraI 4163 TTTAAA TTT^AAA
DraIII 1948 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 715 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3119 GGTGACC G^GT[ACGT]ACC
EcoRI 1319 GAATTC G^AATTC
KpnI 3897 GGTACC GGTAC^C
KspI 28 CCGCGG CCGC^GG
MroI 1286 TCCGGA T^CCGGA
NaeI 1406 GCCGGC GCC^GGC
NcoI 1307 CCATGG C^CATGG
NgoMIV 1406 GCCGGC G^CCGGC
PflMI 477 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 232 ACCGGT A^CCGGT
PmaCI 3862 CACGTG CAC^GTG
PmlI 3862 CACGTG CAC^GTG
Psp1406I 1844 AACGTT AA^CGTT
SacII 28 CCGCGG CCGC^GG
ScaI 2351 AGTACT AGT^ACT
SmaI 3396 CCCGGG CCC^GGG
Van91I 477 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3396 CCCGGG C^CCGGG
XmaI 3396 CCCGGG C^CCGGG
XmnI 1872 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 208 GTCTAC GT^[AC][GT]AC
1527 GTCTAC
AcsI 1319 GAATTC [GA]^AATT[CT]
1876 AAATTC
Aor51HI 2411 AGCGCT AGC^GCT
3246 AGCGCT
ApoI 1319 GAATTC [GA]^AATT[CT]
1876 AAATTC
AvaI 1365 CCCGAG C^[CT]CG[GA]G
3396 CCCGGG
AvrII 3150 CCTAGG C^CTAGG
3643 CCTAGG
BamHI 1979 GGATCC G^GATCC
3809 GGATCC
BciVI 4525 GGATAC GTATCC(6/5)
3507 GTATCC
BglII 2216 AGATCT A^GATCT
4374 AGATCT
BlnI 3150 CCTAGG C^CTAGG
3643 CCTAGG
BlpI 1803 GCTGAGC GC^T[ACGT]AGC
4083 GCTGAGC
Bpu1102I 1803 GCTGAGC GC^T[ACGT]AGC
4083 GCTGAGC
BsaBI 303 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3139 GATTCCCATC
BsaWI 232 ACCGGT [AT]^CCGG[AT]
1286 TCCGGA
BsoBI 1365 CCCGAG C^[CT]CG[GA]G
3396 CCCGGG
Bsp1407I 2516 TGTACA T^GTACA
3949 TGTACA
BspHI 383 TCATGA T^CATGA
4851 TCATGA
BsrGI 2516 TGTACA T^GTACA
3949 TGTACA
CelII 1803 GCTGAGC GC^T[ACGT]AGC
4083 GCTGAGC
Eco47III 2411 AGCGCT AGC^GCT
3246 AGCGCT
EcoT22I 3653 ATGCAT ATGCA^T
4875 ATGCAT
HincII 1072 GTCAAC GT[CT]^[GA]AC
4475 GTTGAC
HindII 1072 GTCAAC GT[CT]^[GA]AC
4475 GTTGAC
MamI 303 GATAGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3139 GATTCCCATC
MslI 2508 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3106 CACCTTGGTG
NsiI 3653 ATGCAT ATGCA^T
4875 ATGCAT
RcaI 383 TCATGA T^CATGA
4851 TCATGA
SapI 2523 GAAGAGC GCTCTTC(1/4)
964 GCTCTTC
SspBI 2516 TGTACA T^GTACA
3949 TGTACA
StuI 441 AGGCCT AGG^CCT
4458 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII