##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccII AclI AflII AlwNI
Aor51HI AscI AseI AsnI AviII
AvrII BamHI BbeI BcgI BfrI
BlnI BsiEI BsiWI BspDI BsrDI
BssHII Bst1107I BstAPI BstUI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I FauI FseI FspI HaeII
KasI KspI MluI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PflMI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2306 GGTACC G^GTACC
AccIII 2363 TCCGGA T^CCGGA
AcyI 2078 GGCGTC G[GA]^CG[CT]C
AgeI 1907 ACCGGT A^CCGGT
AhdI 3164 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2382 GGGCCC GGGCC^C
Asp718I 2306 GGTACC G^GTACC
AspEI 3164 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2713 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2454 CACGTG CAC^GTG
BbvCI 1694 CCTCAGC CCTCAGC(-5/-2)
BciVI 594 GGATAC GTATCC(6/5)
BsaHI 2078 GGCGTC G[GA]^CG[CT]C
BseAI 2363 TCCGGA T^CCGGA
BsmI 4027 GAATGC GAATGC(1/-1)
BspEI 2363 TCCGGA T^CCGGA
BspHI 312 TCATGA T^CATGA
BsrBI 1365 CCGCTC CCGCTC(-3/-3)
BsrFI 1907 ACCGGT [GA]^CCGG[CT]
BssSI 4485 CTCGTG CACGAG(-5/-1)
BstBI 2156 TTCGAA TT^CGAA
BstEII 645 GGTGACC G^GT[ACGT]ACC
BstPI 645 GGTGACC G^GT[ACGT]ACC
Cfr10I 1907 ACCGGT [GA]^CCGG[CT]
DraIII 1049 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1111 GACGCAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3164 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 645 GGTGACC G^GT[ACGT]ACC
EcoT22I 3751 ATGCAT ATGCA^T
Hin1I 2078 GGCGTC G[GA]^CG[CT]C
HpaI 3461 GTTAAC GTT^AAC
KpnI 2306 GGTACC GGTAC^C
MroI 2363 TCCGGA T^CCGGA
NheI 1431 GCTAGC G^CTAGC
NsiI 3751 ATGCAT ATGCA^T
NspV 2156 TTCGAA TT^CGAA
PaeR7I 436 CTCGAG C^TCGAG
PflFI 2713 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1907 ACCGGT A^CCGGT
PmaCI 2454 CACGTG CAC^GTG
PmlI 2454 CACGTG CAC^GTG
RcaI 312 TCATGA T^CATGA
SacI 2697 GAGCTC GAGCT^C
SalI 2719 GTCGAC G^TCGAC
SfuI 2156 TTCGAA TT^CGAA
SphI 4393 GCATGC GCATG^C
Tth111I 2713 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4295 TCTAGA T^CTAGA
XhoI 436 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2719 GTCGAC GT^[AC][GT]AC
3172 GTCTAC
Alw44I 403 GTGCAC G^TGCAC
1504 GTGCAC
AocI 1515 CCTGAGG CC^T[ACGT]AGG
3278 CCTGAGG
ApaLI 403 GTGCAC G^TGCAC
1504 GTGCAC
Asp700I 205 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4202 GAAAACTTTC
AvaI 184 CCCGAG C^[CT]CG[GA]G
436 CTCGAG
BanI 2306 GGTACC G^G[CT][GA]CC
3896 GGCACC
BbsI 1663 GAAGAC GAAGAC(2/6)
4836 GTCTTC
BclI 4664 TGATCA T^GATCA
4731 TGATCA
BcnI 915 CCGGG CC^[GC]GG
1566 CCCGG
BglI 142 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1010 GCCGCAAAGGC
BpuAI 1663 GAAGAC GAAGAC(2/6)
4836 GTCTTC
BsaAI 2454 CACGTG [CT]AC^GT[GA]
2476 TACGTG
BsaBI 726 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4726 GATGTTGATC
BsgI 2335 GTGCAG GTGCAG(16/14)
2761 GTGCAG
BsoBI 184 CCCGAG C^[CT]CG[GA]G
436 CTCGAG
Bsp1407I 1670 TGTACA T^GTACA
3192 TGTACA
BsrGI 1670 TGTACA T^GTACA
3192 TGTACA
Bsu36I 1515 CCTGAGG CC^T[ACGT]AGG
3278 CCTGAGG
CfoI 1689 GCGC GCG^C
2524 GCGC
DraI 700 TTTAAA TTT^AAA
4195 TTTAAA
Eco81I 1515 CCTGAGG CC^T[ACGT]AGG
3278 CCTGAGG
EcoRI 4011 GAATTC G^AATTC
4080 GAATTC
EcoRV 178 GATATC GAT^ATC
4307 GATATC
FbaI 4664 TGATCA T^GATCA
4731 TGATCA
HhaI 1689 GCGC GCG^C
2524 GCGC
HinP1I 1689 GCGC G^CGC
2524 GCGC
MamI 726 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4726 GATGTTGATC
MfeI 1722 CAATTG C^AATTG
3774 CAATTG
MspA1I 993 CCGCTG C[AC]G^C[GT]G
5158 CCGCTG
MunI 1722 CAATTG C^AATTG
3774 CAATTG
NciI 915 CCGGG CC^[GC]GG
1566 CCCGG
SpeI 837 ACTAGT A^CTAGT
3224 ACTAGT
SspBI 1670 TGTACA T^GTACA
3192 TGTACA
SspI 2518 AATATT AAT^ATT
4807 AATATT
XmnI 205 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4202 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BalI
BanII BbvI BfaI BglII BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII