##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AclI AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AspEI
AspI AviII BbrPI BciVI BsiEI
BsiWI BsmBI BspDI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FseI FspI MfeI MluI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SpeI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 646 TCCGGA T^CCGGA
AcyI 3241 GGCGCC G[GA]^CG[CT]C
AflII 2233 CTTAAG C^TTAAG
Asp700I 2273 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4009 CTCGGG C^[CT]CG[GA]G
AvrII 6017 CCTAGG C^CTAGG
BalI 3252 TGGCCA TGG^CCA
BbeI 3241 GGCGCC GGCGC^C
BbvCI 597 GCTGAGG CCTCAGC(-5/-2)
BcgI 3065 CGATCATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2233 CTTAAG C^TTAAG
BglI 455 GCCTGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1892 AGATCT A^GATCT
BlnI 6017 CCTAGG C^CTAGG
BsaBI 567 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3241 GGCGCC G[GA]^CG[CT]C
BsaWI 646 TCCGGA [AT]^CCGG[AT]
BseAI 646 TCCGGA T^CCGGA
BsoBI 4009 CTCGGG C^[CT]CG[GA]G
BspEI 646 TCCGGA T^CCGGA
BspHI 2486 TCATGA T^CATGA
BspLU11I 2736 ACATGT A^CATGT
BspMI 5375 GCAGGT ACCTGC(4/8)
BssSI 5852 CACGAG CACGAG(-5/-1)
BstXI 5559 CCAAATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 953 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 3252 TGGCCA [CT]^GGCC[GA]
EcoO109I 953 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 3356 GATATC GAT^ATC
Hin1I 3241 GGCGCC G[GA]^CG[CT]C
KasI 3241 GGCGCC G^GCGCC
KspI 968 CCGCGG CCGC^GG
MamI 567 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3252 TGGCCA TGG^CCA
MroI 646 TCCGGA T^CCGGA
MscI 3252 TGGCCA TGG^CCA
NarI 3241 GGCGCC GG^CGCC
PacI 3990 TTAATTAA TTAAT^TAA
PpuMI 953 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1250 CAGCTG CAG^CTG
RcaI 2486 TCATGA T^CATGA
SacII 968 CCGCGG CCGC^GG
SfoI 3241 GGCGCC GGC^GCC
SnaBI 1910 TACGTA TAC^GTA
StuI 2518 AGGCCT AGG^CCT
XmnI 2273 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 603 GGTACC G^GTACC
1707 GGTACC
AflIII 1973 ACGTGT A^C[GA][CT]GT
2736 ACATGT
Alw44I 201 GTGCAC G^TGCAC
1977 GTGCAC
ApaLI 201 GTGCAC G^TGCAC
1977 GTGCAC
Asp718I 603 GGTACC G^GTACC
1707 GGTACC
BamHI 112 GGATCC G^GATCC
1204 GGATCC
BlpI 321 GCTCAGC GC^T[ACGT]AGC
1607 GCTCAGC
Bpu1102I 321 GCTCAGC GC^T[ACGT]AGC
1607 GCTCAGC
BsaI 3708 GAGACC GGTCTC(1/5)
6160 GAGACC
BseRI 327 CTCCTC GAGGAG(10/8)
4753 CTCCTC
BsmI 2467 GAATGC GAATGC(1/-1)
2825 GAATGC
Bsp1407I 2186 TGTACA T^GTACA
5780 TGTACA
BsrGI 2186 TGTACA T^GTACA
5780 TGTACA
Bst1107I 3800 GTATAC GTA^TAC
6008 GTATAC
BstZ17I 3800 GTATAC GTA^TAC
6008 GTATAC
CelII 321 GCTCAGC GC^T[ACGT]AGC
1607 GCTCAGC
DsaI 968 CCGCGG C^C[GA][CT]GG
3633 CCGTGG
HaeII 553 AGCGCC [GA]GCGC^[CT]
3241 GGCGCC
HincII 803 GTTAAC GT[CT]^[GA]AC
6184 GTTAAC
HindII 803 GTTAAC GT[CT]^[GA]AC
6184 GTTAAC
HpaI 803 GTTAAC GTT^AAC
6184 GTTAAC
KpnI 603 GGTACC GGTAC^C
1707 GGTACC
PflMI 1535 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3450 CCAAATCATGG
SapI 5857 GAAGAGC GCTCTTC(1/4)
5316 GCTCTTC
SspBI 2186 TGTACA T^GTACA
5780 TGTACA
Van91I 1535 CCACAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3450 CCAAATCATGG
XbaI 1895 TCTAGA T^CTAGA
2772 TCTAGA
XcmI 364 CCATATCTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4405 CCACAACACTACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AseI AsnI
AspHI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII