##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
AocI ApaI AscI AseI AsnI
AspEI AviII BbeI BbrPI BcgI
BciVI BfrI BsaBI BseAI BsiEI
BsiWI BsmBI BspEI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I FseI
FspI KasI KspI MamI MluI
MroI NarI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 5124 GGTACC G^GTACC
AccII 4435 CGCG CG^CG
AcyI 424 GACGCC G[GA]^CG[CT]C
AgeI 3194 ACCGGT A^CCGGT
Aor51HI 4707 AGCGCT AGC^GCT
Asp700I 1012 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5124 GGTACC G^GTACC
AspI 13 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2040 CCTAGG C^CTAGG
BanI 5124 GGTACC G^G[CT][GA]CC
BbvCI 3809 GCTGAGG CCTCAGC(-5/-2)
BclI 3235 TGATCA T^GATCA
BglI 2250 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2040 CCTAGG C^CTAGG
BsaHI 424 GACGCC G[GA]^CG[CT]C
BsaWI 3194 ACCGGT [AT]^CCGG[AT]
BseRI 4665 CTCCTC GAGGAG(10/8)
BspDI 4928 ATCGAT AT^CGAT
BspMI 216 GCAGGT ACCTGC(4/8)
BsrBI 223 GAGCGG CCGCTC(-3/-3)
BstUI 4435 CGCG CG^CG
ClaI 4928 ATCGAT AT^CGAT
DraIII 5190 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4707 AGCGCT AGC^GCT
EcoNI 4278 CCTTGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 225 GCGGG CCCGC(4/6)
FbaI 3235 TGATCA T^GATCA
Hin1I 424 GACGCC G[GA]^CG[CT]C
KpnI 5124 GGTACC GGTAC^C
MspA1I 250 CAGCTG C[AC]G^C[GT]G
MvnI 4435 CGCG CG^CG
NaeI 535 GCCGGC GCC^GGC
NgoMIV 535 GCCGGC G^CCGGC
NheI 4644 GCTAGC G^CTAGC
PflFI 13 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3194 ACCGGT A^CCGGT
PvuII 250 CAGCTG CAG^CTG
SalI 2194 GTCGAC G^TCGAC
SapI 4675 GAAGAGC GCTCTTC(1/4)
SexAI 3117 ACCTGGT A^CC[AT]GGT
SnaBI 2221 TACGTA TAC^GTA
SwaI 3636 ATTTAAAT ATTT^AAAT
Tth111I 13 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1012 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbsI 729 GAAGAC GAAGAC(2/6)
826 GAAGAC
BglII 1652 AGATCT A^GATCT
2794 AGATCT
BpuAI 729 GAAGAC GAAGAC(2/6)
826 GAAGAC
BsaAI 330 TACGTG [CT]AC^GT[GA]
2221 TACGTA
BsmI 1059 GAATGC GAATGC(1/-1)
4357 GAATGC
BspHI 3879 TCATGA T^CATGA
5331 TCATGA
BsrDI 3222 CATTGC GCAATG(2/0)
983 GCAATG
BsrFI 535 GCCGGC [GA]^CCGG[CT]
3194 ACCGGT
BssSI 364 CACGAG CACGAG(-5/-1)
3816 CTCGTG
Cfr10I 535 GCCGGC [GA]^CCGG[CT]
3194 ACCGGT
EcoRI 1330 GAATTC G^AATTC
5558 GAATTC
EcoRV 627 GATATC GAT^ATC
2337 GATATC
HaeII 4628 AGCGCC [GA]GCGC^[CT]
4707 AGCGCT
HindIII 1014 AAGCTT A^AGCTT
1415 AAGCTT
HpaI 2129 GTTAAC GTT^AAC
5594 GTTAAC
MfeI 869 CAATTG C^AATTG
2887 CAATTG
MunI 869 CAATTG C^AATTG
2887 CAATTG
PstI 253 CTGCAG CTGCA^G
4552 CTGCAG
RcaI 3879 TCATGA T^CATGA
5331 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BalI
BamHI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII