##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AclI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AvrII
BbeI BbrPI BcgI BglI BlnI
BsaAI BsaI BsiEI BsiWI BspDI
BspHI BsrFI BssHII BstBI BstEII
BstPI Cfr10I ClaI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI HpaI KasI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI SalI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1815 GACGTC GACGT^C
AflIII 1017 ACATGT A^C[GA][CT]GT
Asp700I 2731 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4318 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1157 GGATCC G^GATCC
BciVI 3194 GGATAC GTATCC(6/5)
BglII 4146 AGATCT A^GATCT
BsaBI 3195 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 1017 ACATGT A^CATGT
BsrBI 3304 GAGCGG CCGCTC(-3/-3)
BssSI 3262 CACGAG CACGAG(-5/-1)
BstXI 647 CCAAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 3559 CGGACCG CG^G[AT]CCG
HincII 774 GTCAAC GT[CT]^[GA]AC
HindII 774 GTCAAC GT[CT]^[GA]AC
KspI 1063 CCGCGG CCGC^GG
MamI 3195 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2309 CATATG CA^TATG
PaeR7I 2117 CTCGAG C^TCGAG
PflFI 4318 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 3559 CGGACCG CG^G[AT]CCG
SacII 1063 CCGCGG CCGC^GG
SapI 4659 GAAGAGC GCTCTTC(1/4)
SbfI 3114 CCTGCAGG CCTGCA^GG
Sse8387I 3114 CCTGCAGG CCTGCA^GG
Tth111I 4318 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2117 CTCGAG C^TCGAG
XmnI 2731 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 643 GGTACC G^GTACC
2015 GGTACC
AccI 1381 GTATAC GT^[AC][GT]AC
3788 GTATAC
AccII 1064 CGCG CG^CG
1417 CGCG
AccIII 854 TCCGGA T^CCGGA
897 TCCGGA
AcyI 1815 GACGTC G[GA]^CG[CT]C
3866 GACGCC
AflII 1642 CTTAAG C^TTAAG
4790 CTTAAG
Asp718I 643 GGTACC G^GTACC
2015 GGTACC
AviII 3005 TGCGCA TGC^GCA
3185 TGCGCA
BalI 1874 TGGCCA TGG^CCA
3467 TGGCCA
BclI 2573 TGATCA T^GATCA
3975 TGATCA
BfrI 1642 CTTAAG C^TTAAG
4790 CTTAAG
BlpI 3108 GCTGAGC GC^T[ACGT]AGC
4006 GCTCAGC
Bpu1102I 3108 GCTGAGC GC^T[ACGT]AGC
4006 GCTCAGC
BsaHI 1815 GACGTC G[GA]^CG[CT]C
3866 GACGCC
BsaWI 854 TCCGGA [AT]^CCGG[AT]
897 TCCGGA
BseAI 854 TCCGGA T^CCGGA
897 TCCGGA
Bsp1407I 383 TGTACA T^GTACA
1898 TGTACA
BspEI 854 TCCGGA T^CCGGA
897 TCCGGA
BsrGI 383 TGTACA T^GTACA
1898 TGTACA
Bst1107I 1381 GTATAC GTA^TAC
3788 GTATAC
BstUI 1064 CGCG CG^CG
1417 CGCG
BstZ17I 1381 GTATAC GTA^TAC
3788 GTATAC
CelII 3108 GCTGAGC GC^T[ACGT]AGC
4006 GCTCAGC
DraIII 160 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4676 CACACTGTG
EcoRI 2125 GAATTC G^AATTC
2167 GAATTC
EcoT22I 198 ATGCAT ATGCA^T
892 ATGCAT
FbaI 2573 TGATCA T^GATCA
3975 TGATCA
FspI 3005 TGCGCA TGC^GCA
3185 TGCGCA
HaeII 2345 GGCGCT [GA]GCGC^[CT]
3308 GGCGCT
Hin1I 1815 GACGTC G[GA]^CG[CT]C
3866 GACGCC
HindIII 1149 AAGCTT A^AGCTT
3623 AAGCTT
KpnI 643 GGTACC GGTAC^C
2015 GGTACC
MluNI 1874 TGGCCA TGG^CCA
3467 TGGCCA
MroI 854 TCCGGA T^CCGGA
897 TCCGGA
MscI 1874 TGGCCA TGG^CCA
3467 TGGCCA
MvnI 1064 CGCG CG^CG
1417 CGCG
NcoI 663 CCATGG C^CATGG
3915 CCATGG
NsiI 198 ATGCAT ATGCA^T
892 ATGCAT
PshAI 23 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1954 GACCAAGGTC
SexAI 455 ACCTGGT A^CC[AT]GGT
2011 ACCAGGT
SspBI 383 TGTACA T^GTACA
1898 TGTACA
StuI 2082 AGGCCT AGG^CCT
2337 AGGCCT
XbaI 1614 TCTAGA T^CTAGA
5108 TCTAGA
XcmI 1595 CCACTGACATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1862 CCACAGCTGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI