################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BclI BfrI BlnI BsaBI BseAI BsiEI BsiWI BspDI BspEI BspMI BsrBI BsrFI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 3944 AACGTT AA^CGTT AcyI 6377 GACGCC G[GA]^CG[CT]C Alw44I 283 GTGCAC G^TGCAC AocI 818 CCTCAGG CC^T[ACGT]AGG ApaLI 283 GTGCAC G^TGCAC AseI 4200 ATTAAT AT^TAAT AsnI 4200 ATTAAT AT^TAAT AspI 5248 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1214 TGGCCA TGG^CCA BcgI 183 CGATGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 3511 CACGTA [CT]AC^GT[GA] BsaHI 6377 GACGCC G[GA]^CG[CT]C BsaWI 763 TCCGGT [AT]^CCGG[AT] BsmBI 2097 GAGACG CGTCTC(1/5) BspHI 5296 TCATGA T^CATGA BssSI 2943 CTCGTG CACGAG(-5/-1) BstAPI 2758 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 818 CCTCAGG CC^T[ACGT]AGG EaeI 1214 TGGCCA [CT]^GGCC[GA] Eco81I 818 CCTCAGG CC^T[ACGT]AGG Hin1I 6377 GACGCC G[GA]^CG[CT]C HincII 6021 GTTAAC GT[CT]^[GA]AC HindII 6021 GTTAAC GT[CT]^[GA]AC HpaI 6021 GTTAAC GTT^AAC MluNI 1214 TGGCCA TGG^CCA MscI 1214 TGGCCA TGG^CCA PacI 3403 TTAATTAA TTAAT^TAA PflFI 5248 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 4200 ATTAAT AT^TAAT Psp1406I 3944 AACGTT AA^CGTT RcaI 5296 TCATGA T^CATGA SapI 4019 GCTCTTC GCTCTTC(1/4) SphI 1975 GCATGC GCATG^C SwaI 5191 ATTTAAAT ATTT^AAAT Tth111I 5248 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Asp700I 1065 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6141 GAAGTTGTTC BanII 1085 GAGCCC G[GA]GC[CT]^C 1693 GAGCCC BbsI 70 GAAGAC GAAGAC(2/6) 1515 GAAGAC BciVI 500 GGATAC GTATCC(6/5) 1767 GTATCC BcnI 341 CCCGG CC^[GC]GG 494 CCGGG BglI 1811 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4112 GCCTCAGAGGC BglII 972 AGATCT A^GATCT 3501 AGATCT BlpI 3065 GCTAAGC GC^T[ACGT]AGC 5775 GCTTAGC Bpu1102I 3065 GCTAAGC GC^T[ACGT]AGC 5775 GCTTAGC BpuAI 70 GAAGAC GAAGAC(2/6) 1515 GAAGAC BssHII 266 GCGCGC G^CGCGC 268 GCGCGC CelII 3065 GCTAAGC GC^T[ACGT]AGC 5775 GCTTAGC EcoNI 3929 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4268 CCTGGGAGAGG EcoRI 557 GAATTC G^AATTC 3001 GAATTC FauI 398 CCCGC CCCGC(4/6) 2585 GCGGG HaeII 2708 AGCGCC [GA]GCGC^[CT] 2815 GGCGCT HgaI 5023 GACGC GACGC(5/10) 6377 GACGC MfeI 5504 CAATTG C^AATTG 6037 CAATTG MspA1I 1597 CAGCTG C[AC]G^C[GT]G 2185 CAGCTG MunI 5504 CAATTG C^AATTG 6037 CAATTG NciI 341 CCCGG CC^[GC]GG 494 CCGGG NdeI 4189 CATATG CA^TATG 4490 CATATG NheI 1629 GCTAGC G^CTAGC 2796 GCTAGC PpuMI 2318 GGGACCT [GA]G^G[AT]CC[CT] 4005 AGGTCCC PshAI 2122 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3036 GACAACGGTC PvuII 1597 CAGCTG CAG^CTG 2185 CAGCTG XbaI 3746 TCTAGA T^CTAGA 4437 TCTAGA XcmI 453 CCAGTGCATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4098 CCAGCAACGGCATGG XmnI 1065 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6141 GAAGTTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BbvI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII