##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BclI BfrI BlnI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BspMI BsrBI BsrFI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FbaI FseI FspI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NgoMIV NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AclI 3944 AACGTT AA^CGTT
AcyI 6377 GACGCC G[GA]^CG[CT]C
Alw44I 283 GTGCAC G^TGCAC
AocI 818 CCTCAGG CC^T[ACGT]AGG
ApaLI 283 GTGCAC G^TGCAC
AseI 4200 ATTAAT AT^TAAT
AsnI 4200 ATTAAT AT^TAAT
AspI 5248 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1214 TGGCCA TGG^CCA
BcgI 183 CGATGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3511 CACGTA [CT]AC^GT[GA]
BsaHI 6377 GACGCC G[GA]^CG[CT]C
BsaWI 763 TCCGGT [AT]^CCGG[AT]
BsmBI 2097 GAGACG CGTCTC(1/5)
BspHI 5296 TCATGA T^CATGA
BssSI 2943 CTCGTG CACGAG(-5/-1)
BstAPI 2758 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 818 CCTCAGG CC^T[ACGT]AGG
EaeI 1214 TGGCCA [CT]^GGCC[GA]
Eco81I 818 CCTCAGG CC^T[ACGT]AGG
Hin1I 6377 GACGCC G[GA]^CG[CT]C
HincII 6021 GTTAAC GT[CT]^[GA]AC
HindII 6021 GTTAAC GT[CT]^[GA]AC
HpaI 6021 GTTAAC GTT^AAC
MluNI 1214 TGGCCA TGG^CCA
MscI 1214 TGGCCA TGG^CCA
PacI 3403 TTAATTAA TTAAT^TAA
PflFI 5248 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4200 ATTAAT AT^TAAT
Psp1406I 3944 AACGTT AA^CGTT
RcaI 5296 TCATGA T^CATGA
SapI 4019 GCTCTTC GCTCTTC(1/4)
SphI 1975 GCATGC GCATG^C
SwaI 5191 ATTTAAAT ATTT^AAAT
Tth111I 5248 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Asp700I 1065 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6141 GAAGTTGTTC
BanII 1085 GAGCCC G[GA]GC[CT]^C
1693 GAGCCC
BbsI 70 GAAGAC GAAGAC(2/6)
1515 GAAGAC
BciVI 500 GGATAC GTATCC(6/5)
1767 GTATCC
BcnI 341 CCCGG CC^[GC]GG
494 CCGGG
BglI 1811 GCCTGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4112 GCCTCAGAGGC
BglII 972 AGATCT A^GATCT
3501 AGATCT
BlpI 3065 GCTAAGC GC^T[ACGT]AGC
5775 GCTTAGC
Bpu1102I 3065 GCTAAGC GC^T[ACGT]AGC
5775 GCTTAGC
BpuAI 70 GAAGAC GAAGAC(2/6)
1515 GAAGAC
BssHII 266 GCGCGC G^CGCGC
268 GCGCGC
CelII 3065 GCTAAGC GC^T[ACGT]AGC
5775 GCTTAGC
EcoNI 3929 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4268 CCTGGGAGAGG
EcoRI 557 GAATTC G^AATTC
3001 GAATTC
FauI 398 CCCGC CCCGC(4/6)
2585 GCGGG
HaeII 2708 AGCGCC [GA]GCGC^[CT]
2815 GGCGCT
HgaI 5023 GACGC GACGC(5/10)
6377 GACGC
MfeI 5504 CAATTG C^AATTG
6037 CAATTG
MspA1I 1597 CAGCTG C[AC]G^C[GT]G
2185 CAGCTG
MunI 5504 CAATTG C^AATTG
6037 CAATTG
NciI 341 CCCGG CC^[GC]GG
494 CCGGG
NdeI 4189 CATATG CA^TATG
4490 CATATG
NheI 1629 GCTAGC G^CTAGC
2796 GCTAGC
PpuMI 2318 GGGACCT [GA]G^G[AT]CC[CT]
4005 AGGTCCC
PshAI 2122 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3036 GACAACGGTC
PvuII 1597 CAGCTG CAG^CTG
2185 CAGCTG
XbaI 3746 TCTAGA T^CTAGA
4437 TCTAGA
XcmI 453 CCAGTGCATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4098 CCAGCAACGGCATGG
XmnI 1065 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6141 GAAGTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BbvI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII