##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AscI Asp718I AvrII BfrI
BlnI BseAI BspEI BspLU11I BsrDI
BssHII Bst1107I BstZ17I CpoI DrdI
EagI EclXI Eco52I EcoRV FseI
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NheI NotI
NruI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SbfI
SfiI SgrAI SpeI Sse8387I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AocI 3007 CCTGAGG CC^T[ACGT]AGG
Aor51HI 114 AGCGCT AGC^GCT
ApaI 4083 GGGCCC GGGCC^C
AseI 1635 ATTAAT AT^TAAT
AsnI 1635 ATTAAT AT^TAAT
AspI 2757 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4904 TGCGCA TGC^GCA
BamHI 5630 GGATCC G^GATCC
BbrPI 5256 CACGTG CAC^GTG
BbvCI 3839 GCTGAGG CCTCAGC(-5/-2)
BcgI 5455 GCACGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 6189 GCCTTAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 3651 GAGACC GGTCTC(1/5)
BsaWI 1127 ACCGGA [AT]^CCGG[AT]
BsiEI 5465 CGACCG CG[GA][CT]^CG
BsiWI 5144 CGTACG C^GTACG
BsmBI 2350 CGTCTC CGTCTC(1/5)
BsmI 5149 GAATGC GAATGC(1/-1)
BspDI 5978 ATCGAT AT^CGAT
BstBI 4041 TTCGAA TT^CGAA
Bsu36I 3007 CCTGAGG CC^T[ACGT]AGG
ClaI 5978 ATCGAT AT^CGAT
DraIII 5910 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 114 AGCGCT AGC^GCT
Eco81I 3007 CCTGAGG CC^T[ACGT]AGG
EcoRI 957 GAATTC G^AATTC
FspI 4904 TGCGCA TGC^GCA
HincII 1311 GTCGAC GT[CT]^[GA]AC
HindII 1311 GTCGAC GT[CT]^[GA]AC
NspV 4041 TTCGAA TT^CGAA
PacI 4067 TTAATTAA TTAAT^TAA
PflFI 2757 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5256 CACGTG CAC^GTG
PmlI 5256 CACGTG CAC^GTG
PshBI 1635 ATTAAT AT^TAAT
SalI 1311 GTCGAC G^TCGAC
SexAI 6368 ACCAGGT A^CC[AT]GGT
SfuI 4041 TTCGAA TT^CGAA
SmaI 854 CCCGGG CCC^GGG
SnaBI 5059 TACGTA TAC^GTA
SphI 2030 GCATGC GCATG^C
SspI 2881 AATATT AAT^ATT
StuI 5021 AGGCCT AGG^CCT
SwaI 5982 ATTTAAAT ATTT^AAAT
Tth111I 2757 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 854 CCCGGG C^CCGGG
XmaI 854 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1311 GTCGAC GT^[AC][GT]AC
3416 GTAGAC
AccII 14 CGCG CG^CG
82 CGCG
AlwNI 893 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
1900 CAGTAGCTG
BclI 203 TGATCA T^GATCA
6220 TGATCA
BglII 4485 AGATCT A^GATCT
5708 AGATCT
BlpI 363 GCTGAGC GC^T[ACGT]AGC
2206 GCTCAGC
Bpu1102I 363 GCTGAGC GC^T[ACGT]AGC
2206 GCTCAGC
BsaBI 859 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1641 GATTTTCATC
BssSI 5442 CACGAG CACGAG(-5/-1)
5508 CACGAG
BstEII 2119 GGTGACC G^GT[ACGT]ACC
3582 GGTGACC
BstPI 2119 GGTGACC G^GT[ACGT]ACC
3582 GGTGACC
BstUI 14 CGCG CG^CG
82 CGCG
CelII 363 GCTGAGC GC^T[ACGT]AGC
2206 GCTCAGC
DraI 5729 TTTAAA TTT^AAA
5983 TTTAAA
EcoO65I 2119 GGTGACC G^GT[ACGT]ACC
3582 GGTGACC
EcoT22I 5087 ATGCAT ATGCA^T
5151 ATGCAT
FbaI 203 TGATCA T^GATCA
6220 TGATCA
MamI 859 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1641 GATTTTCATC
MvnI 14 CGCG CG^CG
82 CGCG
NaeI 476 GCCGGC GCC^GGC
4857 GCCGGC
NdeI 2101 CATATG CA^TATG
5084 CATATG
NgoMIV 476 GCCGGC G^CCGGC
4857 GCCGGC
NsiI 5087 ATGCAT ATGCA^T
5151 ATGCAT
NspI 2030 GCATGC [GA]CATG^[CT]
5544 ACATGC
PstI 1252 CTGCAG CTGCA^G
4975 CTGCAG
SapI 381 GCTCTTC GCTCTTC(1/4)
4694 GCTCTTC
ScaI 2681 AGTACT AGT^ACT
5422 AGTACT
XcmI 1201 CCACACAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4308 CCAGCCTCTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AhdI AluI Alw44I AlwI ApaLI
ApoI Asp700I AspEI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
PaeR7I PflMI PleI PpuMI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoI XhoII XmnI