##################################
# NO CUT ENZYMES (   52 enzymes) #
##################################

AatII       AclI        AcsI        AgeI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
AvrII       BciVI       BclI        BglII       BlnI        
BlpI        Bpu1102I    BsiWI       BsmBI       BspHI       
Bst1107I    BstZ17I     CelII       DraI        EcoRI       
EcoRV       EcoT22I     FbaI        HincII      HindII      
HindIII     HpaI        MfeI        MluI        MunI        
NotI        NsiI        PacI        PinAI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        SalI        
SexAI       SfiI        SnaBI       SpeI        SphI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I       5406  GGTACC               G^GTACC
AccI         4196  GTCTAC               GT^[AC][GT]AC
AccIII        458  TCCGGA               T^CCGGA
AhdI         3236  GACATACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I      1673  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      5406  GGTACC               G^GTACC
AspEI        3236  GACATACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI        1886  CACGTG               CAC^GTG
BsaAI        1886  CACGTG               [CT]AC^GT[GA]
BsaBI         195  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         458  TCCGGA               T^CCGGA
BsmI          703  GAATGC               GAATGC(1/-1)
BspDI        1614  ATCGAT               AT^CGAT
BspEI         458  TCCGGA               T^CCGGA
BssSI        1358  CTCGTG               CACGAG(-5/-1)
BstBI        4370  TTCGAA               TT^CGAA
ClaI         1614  ATCGAT               AT^CGAT
CpoI         1533  CGGACCG              CG^G[AT]CCG
DrdI         5009  GACCAGGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3950  CGGCCG               C^GGCCG
Eam1105I     3236  GACATACCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3950  CGGCCG               C^GGCCG
Eco52I       3950  CGGCCG               C^GGCCG
FseI         3947  GGCCGGCC             GGCCGG^CC
KpnI         5406  GGTACC               GGTAC^C
MamI          195  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          458  TCCGGA               T^CCGGA
NdeI         4893  CATATG               CA^TATG
NheI         4841  GCTAGC               G^CTAGC
NruI         2563  TCGCGA               TCG^CGA
NspV         4370  TTCGAA               TT^CGAA
PaeR7I       2649  CTCGAG               C^TCGAG
PmaCI        1886  CACGTG               CAC^GTG
PmlI         1886  CACGTG               CAC^GTG
PshAI        3846  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        1533  CGGACCG              CG^G[AT]CCG
ScaI         2106  AGTACT               AGT^ACT
SfuI         4370  TTCGAA               TT^CGAA
SgrAI         156  CGCCGGCG             C[GA]^CCGG[CT]G
SspI         5483  AATATT               AAT^ATT
XhoI         2649  CTCGAG               C^TCGAG
XmnI         1673  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Aor51HI       508  AGCGCT               AGC^GCT
             2969  AGCGCT              
AspI         1165  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4439  GACCCTGTC           
AviII        1510  TGCGCA               TGC^GCA
             3186  TGCGCA              
BsaWI         458  TCCGGA               [AT]^CCGG[AT]
             4002  TCCGGT              
BsiEI        3105  CGACCG               CG[GA][CT]^CG
             3950  CGGCCG              
BspLU11I     2535  ACATGT               A^CATGT
             5546  ACATGT              
BssHII       2018  GCGCGC               G^CGCGC
             2037  GCGCGC              
BstEII       2319  GGTCACC              G^GT[ACGT]ACC
             3019  GGTGACC             
BstPI        2319  GGTCACC              G^GT[ACGT]ACC
             3019  GGTGACC             
DraIII       2424  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4296  CACCCAGTG           
Eco47III      508  AGCGCT               AGC^GCT
             2969  AGCGCT              
EcoO65I      2319  GGTCACC              G^GT[ACGT]ACC
             3019  GGTGACC             
FspI         1510  TGCGCA               TGC^GCA
             3186  TGCGCA              
KspI            8  CCGCGG               CCGC^GG
             1824  CCGCGG              
NspI         2535  ACATGT               [GA]CATG^[CT]
             5546  ACATGT              
PflFI        1165  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4439  GACCCTGTC           
SacII           8  CCGCGG               CCGC^GG
             1824  CCGCGG              
SapI         3481  GAAGAGC              GCTCTTC(1/4)
             4951  GAAGAGC             
Tsp509I       391  AATT                 ^AATT
             1722  AATT                
Tth111I      1165  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4439  GACCCTGTC           
XcmI          904  CCACGGCTGGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5297  CCAGGCTGCGGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflII       
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        AspHI       AvaI        AvaII       BalI        
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcgI        BcnI        BfaI        
BfrI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      SbfI        ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        Sse8387I    SspBI       
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       XmaCI       XmaI