##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AcsI AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
AvrII BciVI BclI BglII BlnI
BlpI Bpu1102I BsiWI BsmBI BspHI
Bst1107I BstZ17I CelII DraI EcoRI
EcoRV EcoT22I FbaI HincII HindII
HindIII HpaI MfeI MluI MunI
NotI NsiI PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI SalI
SexAI SfiI SnaBI SpeI SphI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 5406 GGTACC G^GTACC
AccI 4196 GTCTAC GT^[AC][GT]AC
AccIII 458 TCCGGA T^CCGGA
AhdI 3236 GACATACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1673 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5406 GGTACC G^GTACC
AspEI 3236 GACATACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1886 CACGTG CAC^GTG
BsaAI 1886 CACGTG [CT]AC^GT[GA]
BsaBI 195 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 458 TCCGGA T^CCGGA
BsmI 703 GAATGC GAATGC(1/-1)
BspDI 1614 ATCGAT AT^CGAT
BspEI 458 TCCGGA T^CCGGA
BssSI 1358 CTCGTG CACGAG(-5/-1)
BstBI 4370 TTCGAA TT^CGAA
ClaI 1614 ATCGAT AT^CGAT
CpoI 1533 CGGACCG CG^G[AT]CCG
DrdI 5009 GACCAGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3950 CGGCCG C^GGCCG
Eam1105I 3236 GACATACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3950 CGGCCG C^GGCCG
Eco52I 3950 CGGCCG C^GGCCG
FseI 3947 GGCCGGCC GGCCGG^CC
KpnI 5406 GGTACC GGTAC^C
MamI 195 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 458 TCCGGA T^CCGGA
NdeI 4893 CATATG CA^TATG
NheI 4841 GCTAGC G^CTAGC
NruI 2563 TCGCGA TCG^CGA
NspV 4370 TTCGAA TT^CGAA
PaeR7I 2649 CTCGAG C^TCGAG
PmaCI 1886 CACGTG CAC^GTG
PmlI 1886 CACGTG CAC^GTG
PshAI 3846 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1533 CGGACCG CG^G[AT]CCG
ScaI 2106 AGTACT AGT^ACT
SfuI 4370 TTCGAA TT^CGAA
SgrAI 156 CGCCGGCG C[GA]^CCGG[CT]G
SspI 5483 AATATT AAT^ATT
XhoI 2649 CTCGAG C^TCGAG
XmnI 1673 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 508 AGCGCT AGC^GCT
2969 AGCGCT
AspI 1165 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4439 GACCCTGTC
AviII 1510 TGCGCA TGC^GCA
3186 TGCGCA
BsaWI 458 TCCGGA [AT]^CCGG[AT]
4002 TCCGGT
BsiEI 3105 CGACCG CG[GA][CT]^CG
3950 CGGCCG
BspLU11I 2535 ACATGT A^CATGT
5546 ACATGT
BssHII 2018 GCGCGC G^CGCGC
2037 GCGCGC
BstEII 2319 GGTCACC G^GT[ACGT]ACC
3019 GGTGACC
BstPI 2319 GGTCACC G^GT[ACGT]ACC
3019 GGTGACC
DraIII 2424 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4296 CACCCAGTG
Eco47III 508 AGCGCT AGC^GCT
2969 AGCGCT
EcoO65I 2319 GGTCACC G^GT[ACGT]ACC
3019 GGTGACC
FspI 1510 TGCGCA TGC^GCA
3186 TGCGCA
KspI 8 CCGCGG CCGC^GG
1824 CCGCGG
NspI 2535 ACATGT [GA]CATG^[CT]
5546 ACATGT
PflFI 1165 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4439 GACCCTGTC
SacII 8 CCGCGG CCGC^GG
1824 CCGCGG
SapI 3481 GAAGAGC GCTCTTC(1/4)
4951 GAAGAGC
Tsp509I 391 AATT ^AATT
1722 AATT
Tth111I 1165 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4439 GACCCTGTC
XcmI 904 CCACGGCTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5297 CCAGGCTGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BfrI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SfoI SmaI SmlI Sse8387I SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XhoII XmaCI XmaI