################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AatII AclI AcsI AgeI Alw44I ApaLI ApoI AscI AseI AsnI AvrII BciVI BclI BglII BlnI BlpI Bpu1102I BsiWI BsmBI BspHI Bst1107I BstZ17I CelII DraI EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI MfeI MluI MunI NotI NsiI PacI PinAI PmeI PshBI Psp1406I PvuI RcaI SalI SexAI SfiI SnaBI SpeI SphI SwaI XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 5406 GGTACC G^GTACC AccI 4196 GTCTAC GT^[AC][GT]AC AccIII 458 TCCGGA T^CCGGA AhdI 3236 GACATACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 1673 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 5406 GGTACC G^GTACC AspEI 3236 GACATACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1886 CACGTG CAC^GTG BsaAI 1886 CACGTG [CT]AC^GT[GA] BsaBI 195 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 458 TCCGGA T^CCGGA BsmI 703 GAATGC GAATGC(1/-1) BspDI 1614 ATCGAT AT^CGAT BspEI 458 TCCGGA T^CCGGA BssSI 1358 CTCGTG CACGAG(-5/-1) BstBI 4370 TTCGAA TT^CGAA ClaI 1614 ATCGAT AT^CGAT CpoI 1533 CGGACCG CG^G[AT]CCG DrdI 5009 GACCAGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3950 CGGCCG C^GGCCG Eam1105I 3236 GACATACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3950 CGGCCG C^GGCCG Eco52I 3950 CGGCCG C^GGCCG FseI 3947 GGCCGGCC GGCCGG^CC KpnI 5406 GGTACC GGTAC^C MamI 195 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 458 TCCGGA T^CCGGA NdeI 4893 CATATG CA^TATG NheI 4841 GCTAGC G^CTAGC NruI 2563 TCGCGA TCG^CGA NspV 4370 TTCGAA TT^CGAA PaeR7I 2649 CTCGAG C^TCGAG PmaCI 1886 CACGTG CAC^GTG PmlI 1886 CACGTG CAC^GTG PshAI 3846 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 1533 CGGACCG CG^G[AT]CCG ScaI 2106 AGTACT AGT^ACT SfuI 4370 TTCGAA TT^CGAA SgrAI 156 CGCCGGCG C[GA]^CCGG[CT]G SspI 5483 AATATT AAT^ATT XhoI 2649 CTCGAG C^TCGAG XmnI 1673 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Aor51HI 508 AGCGCT AGC^GCT 2969 AGCGCT AspI 1165 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4439 GACCCTGTC AviII 1510 TGCGCA TGC^GCA 3186 TGCGCA BsaWI 458 TCCGGA [AT]^CCGG[AT] 4002 TCCGGT BsiEI 3105 CGACCG CG[GA][CT]^CG 3950 CGGCCG BspLU11I 2535 ACATGT A^CATGT 5546 ACATGT BssHII 2018 GCGCGC G^CGCGC 2037 GCGCGC BstEII 2319 GGTCACC G^GT[ACGT]ACC 3019 GGTGACC BstPI 2319 GGTCACC G^GT[ACGT]ACC 3019 GGTGACC DraIII 2424 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 4296 CACCCAGTG Eco47III 508 AGCGCT AGC^GCT 2969 AGCGCT EcoO65I 2319 GGTCACC G^GT[ACGT]ACC 3019 GGTGACC FspI 1510 TGCGCA TGC^GCA 3186 TGCGCA KspI 8 CCGCGG CCGC^GG 1824 CCGCGG NspI 2535 ACATGT [GA]CATG^[CT] 5546 ACATGT PflFI 1165 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4439 GACCCTGTC SacII 8 CCGCGG CCGC^GG 1824 CCGCGG SapI 3481 GAAGAGC GCTCTTC(1/4) 4951 GAAGAGC Tsp509I 391 AATT ^AATT 1722 AATT Tth111I 1165 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4439 GACCCTGTC XcmI 904 CCACGGCTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5297 CCAGGCTGCGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AciI AcyI AfaI AflII AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BfrI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfoI SmaI SmlI Sse8387I SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI