##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AclI Aor51HI ApaI
AscI AseI AsnI AspI AvaI
AviII AvrII BbeI BbrPI BcgI
BlnI BlpI Bpu1102I BsaAI BsiEI
BsiWI BsoBI BspDI BsrBI BssHII
BssSI Bst1107I BstUI BstXI BstZ17I
CelII ClaI CpoI DraII DsaI
EagI EclXI Eco47III Eco52I EcoO109I
EcoRV FseI FspI HaeII KasI
KspI MaeII MluI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2047 GGTACC G^GTACC
AccI 4148 GTAGAC GT^[AC][GT]AC
AcyI 213 GGCGTC G[GA]^CG[CT]C
AflII 1153 CTTAAG C^TTAAG
AgeI 1488 ACCGGT A^CCGGT
Asp718I 2047 GGTACC G^GTACC
BamHI 3917 GGATCC G^GATCC
BbvCI 2928 GCTGAGG CCTCAGC(-5/-2)
BfrI 1153 CTTAAG C^TTAAG
BsaBI 5044 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 213 GGCGTC G[GA]^CG[CT]C
BsmBI 2917 GAGACG CGTCTC(1/5)
BsrFI 1488 ACCGGT [GA]^CCGG[CT]
BstBI 1760 TTCGAA TT^CGAA
Cfr10I 1488 ACCGGT [GA]^CCGG[CT]
DrdI 3923 GACACCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2254 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 213 GGCGTC G[GA]^CG[CT]C
HindIII 5054 AAGCTT A^AGCTT
HpaI 2191 GTTAAC GTT^AAC
KpnI 2047 GGTACC GGTAC^C
MamI 5044 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3698 CAATTG C^AATTG
MunI 3698 CAATTG C^AATTG
NdeI 2042 CATATG CA^TATG
NspV 1760 TTCGAA TT^CGAA
PinAI 1488 ACCGGT A^CCGGT
PshAI 2584 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4127 GAGCTC GAGCT^C
ScaI 4857 AGTACT AGT^ACT
SexAI 4928 ACCAGGT A^CC[AT]GGT
SfuI 1760 TTCGAA TT^CGAA
SpeI 5273 ACTAGT A^CTAGT
SphI 1619 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1041 TCCGGA T^CCGGA
2207 TCCGGA
AflIII 2968 ACATGT A^C[GA][CT]GT
4087 ACATGT
AhdI 2407 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2476 GACTCCATGTC
Alw44I 3014 GTGCAC G^TGCAC
4417 GTGCAC
ApaLI 3014 GTGCAC G^TGCAC
4417 GTGCAC
AspEI 2407 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2476 GACTCCATGTC
BglI 1945 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4677 GCCTTACTGGC
BsaI 4115 GAGACC GGTCTC(1/5)
3580 GGTCTC
BseAI 1041 TCCGGA T^CCGGA
2207 TCCGGA
BspEI 1041 TCCGGA T^CCGGA
2207 TCCGGA
BspLU11I 2968 ACATGT A^CATGT
4087 ACATGT
BstAPI 2685 GCATGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3947 GCACTTCATGC
CfoI 3612 GCGC GCG^C
4672 GCGC
DraIII 2158 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3877 CACATGGTG
Eam1105I 2407 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2476 GACTCCATGTC
HhaI 3612 GCGC GCG^C
4672 GCGC
HinP1I 3612 GCGC G^CGC
4672 GCGC
HincII 2191 GTTAAC GT[CT]^[GA]AC
2473 GTTGAC
HindII 2191 GTTAAC GT[CT]^[GA]AC
2473 GTTGAC
MroI 1041 TCCGGA T^CCGGA
2207 TCCGGA
PflMI 2517 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3276 CCATAGCTTGG
SapI 5538 GAAGAGC GCTCTTC(1/4)
1420 GCTCTTC
StuI 696 AGGCCT AGG^CCT
5774 AGGCCT
Van91I 2517 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3276 CCATAGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI EaeI EarI
Eco81I EcoO65I EcoRI EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoII XmnI