##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AscI AseI AsnI Asp718I
AvrII BbrPI BclI BfrI BglII
BlnI BsaWI BseAI BsiWI BspEI
BspHI BspLU11I BsrBI BsrDI Bst1107I
BstEII BstPI BstZ17I CpoI EcoO65I
EcoRV FbaI FseI HindIII HpaI
KpnI MluI MroI NaeI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SfiI SgrAI SnaBI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1599 GACGTC GACGT^C
AccI 4906 GTCTAC GT^[AC][GT]AC
AhdI 4450 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4450 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 109 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2456 TGCGCA TGC^GCA
BbeI 2660 GGCGCC GGCGC^C
BbvCI 900 CCTCAGC CCTCAGC(-5/-2)
BciVI 167 GGATAC GTATCC(6/5)
BlpI 25 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 25 GCTTAGC GC^T[ACGT]AGC
BsaAI 2565 TACGTG [CT]AC^GT[GA]
BsaBI 5327 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 4309 CGGCCG CG[GA][CT]^CG
BsrFI 3496 ACCGGC [GA]^CCGG[CT]
BssHII 142 GCGCGC G^CGCGC
BstBI 3935 TTCGAA TT^CGAA
CelII 25 GCTTAGC GC^T[ACGT]AGC
Cfr10I 3496 ACCGGC [GA]^CCGG[CT]
DraI 5089 TTTAAA TTT^AAA
EagI 4309 CGGCCG C^GGCCG
Eam1105I 4450 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4309 CGGCCG C^GGCCG
Eco52I 4309 CGGCCG C^GGCCG
FspI 2456 TGCGCA TGC^GCA
KasI 2660 GGCGCC G^GCGCC
KspI 2839 CCGCGG CCGC^GG
MamI 5327 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4373 CAATTG C^AATTG
MunI 4373 CAATTG C^AATTG
NarI 2660 GGCGCC GG^CGCC
NcoI 1346 CCATGG C^CATGG
NdeI 4951 CATATG CA^TATG
NspV 3935 TTCGAA TT^CGAA
PflFI 109 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1913 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2839 CCGCGG CCGC^GG
ScaI 5470 AGTACT AGT^ACT
SexAI 2511 ACCTGGT A^CC[AT]GGT
SfoI 2660 GGCGCC GGC^GCC
SfuI 3935 TTCGAA TT^CGAA
SmaI 2554 CCCGGG CCC^GGG
SmlI 1633 CTTGAG C^T[CT][GA]AG
Tth111I 109 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2554 CCCGGG C^CCGGG
XmaI 2554 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 1599 GACGTC G[GA]^CG[CT]C
2660 GGCGCC
AocI 19 CCTGAGG CC^T[ACGT]AGG
1112 CCTCAGG
Aor51HI 73 AGCGCT AGC^GCT
4193 AGCGCT
BsaHI 1599 GACGTC G[GA]^CG[CT]C
2660 GGCGCC
BsgI 2706 CTGCAC GTGCAG(16/14)
1037 GTGCAG
BsmBI 2528 CGTCTC CGTCTC(1/5)
2616 GAGACG
Bsp1407I 487 TGTACA T^GTACA
5522 TGTACA
BspDI 3037 ATCGAT AT^CGAT
3275 ATCGAT
BsrGI 487 TGTACA T^GTACA
5522 TGTACA
BssSI 5555 CACGAG CACGAG(-5/-1)
2531 CTCGTG
Bsu36I 19 CCTGAGG CC^T[ACGT]AGG
1112 CCTCAGG
ClaI 3037 ATCGAT AT^CGAT
3275 ATCGAT
DraIII 156 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
5451 CACACAGTG
Eco47III 73 AGCGCT AGC^GCT
4193 AGCGCT
Eco81I 19 CCTGAGG CC^T[ACGT]AGG
1112 CCTCAGG
FauI 2838 CCCGC CCCGC(4/6)
3773 CCCGC
Hin1I 1599 GACGTC G[GA]^CG[CT]C
2660 GGCGCC
NheI 76 GCTAGC G^CTAGC
3094 GCTAGC
SacI 4701 GAGCTC GAGCT^C
5345 GAGCTC
SapI 1964 GAAGAGC GCTCTTC(1/4)
1362 GCTCTTC
SpeI 1136 ACTAGT A^CTAGT
4384 ACTAGT
SspBI 487 TGTACA T^GTACA
5522 TGTACA
StuI 1637 AGGCCT AGG^CCT
1853 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI